LOCUS       sequence-404191-       13816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1888
FEATURES             Location/Qualifiers
     source          1..13816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9673..10261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9726..9746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10144..10155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10333..10566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10644..10765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10681..10700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10735..10754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10805..10940
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10960..10979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10961..10979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10989..11006)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10989..11005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10998..11020)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11147..11602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11234..11253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11444..11465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11628..11732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11733..11801,11802..12593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11951..11970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12764..13352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13253..13272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13506..13523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13640..13661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13676..13681,13682..13699,13700..13706)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13714..13730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13719..13741
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13738..13754
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13738..13754
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13773..13793
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13775..13793
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgctg aaacttctca accagaagaa
     4141 agggccttca cagtgtcctt tatgtaagaa tgatataacc aaaaggagcc tacaagaaag
     4201 tacgagattt agtcaacttg ttgaagagct attgaaaatc atttgtgctt ttcagcttga
     4261 cacaggtttg gagtatgcaa acagctataa ttttgcaaaa aaggaaaata actctcctga
     4321 acatctaaaa gatgaagttt ctatcatcca aagtatgggc tacagaaacc gtgccaaaag
     4381 acttctacag agtgaacccg aaaatccttc cttgcaggaa accagtctca gtgtccaact
     4441 ctctaacctt ggaactgtga gaactctgag gacaaagcag cggatacaac ctcaaaagac
     4501 gtctgtctac attgaattgg gatctgattc ttctgaagat accgttaata aggcaactta
     4561 ttgcagtgtg ggagatcaag aattgttaca aatcacccct caaggaacca gggatgaaat
     4621 cagtttggat tctgcaaaaa aggctgcttg tgaattttct gagacggatg taacaaatac
     4681 tgaacatcat caacccagta ataatgattt gaacaccact gagaagcgtg cagctgagag
     4741 gcatccagaa aagtatcagg gtagttctgt ttcaaacttg catgtggagc catgtggcac
     4801 aaatactcat gccagctcat tacagcatga gaacagcagt ttattactca ctaaagacag
     4861 aatgaatgta gaaaaggctg aattctgtaa taaaagcaaa cagcctggct tagcaaggag
     4921 ccaacataac agatgggctg gaagtaagga aacatgtaat gataggcgga ctcccagcac
     4981 agaaaaaaag gtagatctga atgctgatcc cctgtgtgag agaaaagaat ggaataagca
     5041 gaaactgcca tgctcagaga atcctagaga tactgaagat gttccttgga taacactaaa
     5101 tagcagcatt cagaaagtta atgagtggtt ttccagaagt gatgaactgt taggttctga
     5161 tgactcacat gatggggagt ctgaatcaaa tgccaaagta gctgatgtat tggacgttct
     5221 aaatgaggta gatgaatatt ctggttcttc agagaaaata gacttactgg ccagtgatcc
     5281 tcatgaggct ttaatatgta aaagtgaaag agttcactcc aaatcagtag agagtaatat
     5341 tgaagacaaa atatttggga aaacctatcg gaagaaggca agcctcccca acttaagcca
     5401 tgtaactgaa aatctaatta taggagcatt tgttactgag ccacagataa tacaagagcg
     5461 tcccctcaca aataaattaa agcgtaaaag gagacctaca tcaggccttc atcctgagga
     5521 ttttatcaag aaagcagatt tggcagttca aaagactcct gaaatgataa atcagggaac
     5581 taaccaaacg gagcagaatg gtcaagtgat gaatattact aatagtggtc atgagaataa
     5641 aacaaaaggt gattctattc agaatgagaa aaatcctaac ccaatagaat cactcgaaaa
     5701 agaatctgct ttcaaaacga aagctgaacc tataagcagc agtataagca atatggaact
     5761 cgaattaaat atccacaatt caaaagcacc taaaaagaat aggctgagga ggaagtcttc
     5821 taccaggcat attcatgcgc ttgaactagt agtcagtaga aatctaagcc cacctaattg
     5881 tactgaattg caaattgata gttgttctag cagtgaagag ataaagaaaa aaaagtacaa
     5941 ccaaatgcca gtcaggcaca gcagaaacct acaactcatg gaaggtaaag aacctgcaac
     6001 tggagccaag aagagtaaca agccaaatga acagacaagt aaaagacatg acagcgatac
     6061 tttcccagag ctgaagttaa caaatgcacc tggttctttt actaagtgtt caaataccag
     6121 tgaacttaaa gaatttgtca atcctagcct tccaagagaa gaaaaagaag agaaactaga
     6181 aacagttaaa gtgtctaata atgctgaaga ccccaaagat ctcatgttaa gtggagaaag
     6241 ggttttgcaa actgaaagat ctgtagagag tagcagtatt tcattggtac ctggtactga
     6301 ttatggcact caggaaagta tctcgttact ggaagttagc actctaggga aggcaaaaac
     6361 agaaccaaat aaatgtgtga gtcagtgtgc agcatttgaa aaccccaagg gactaattca
     6421 tggttgttcc aaagataata gaaatgacac agaaggcttt aagtatccat tgggacatga
     6481 agttaaccac agtcgggaaa caagcataga aatggaagaa agtgaacttg atgctcagta
     6541 tttgcagaat acattcaagg tttcaaagcg ccagtcattt gctccgtttt caaatccagg
     6601 aaatgcagaa gaggaatgtg caacattctc tgcccactct gggtccttaa agaaacaaag
     6661 tccaaaagtc acttttgaat gtgaacaaaa ggaagaaaat caaggaaaga atgagtctaa
     6721 tatcaagcct gtacagacag ttaatatcac tgcaggcttt cctgtggttg gtcagaaaga
     6781 taagccagtt gataatgcca aatgtagtat caaaggaggc tctaggtttt gtctatcatc
     6841 tcagttcaga ggcaacgaaa ctggactcat tactccaaat aaacatggac ttttacaaaa
     6901 cccatatcgt ataccaccac tttttcccat caagtcattt gttaaaacta aatgtaagaa
     6961 aaatctgcta gaggaaaact ttgaggaaca ttcaatgtca cctgaaagag aaatgggaaa
     7021 tgagaacatt ccaagtacag tgagcacaat tagccgtaat aacattagag aaaatgtttt
     7081 taaagaagcc agctcaagca atattaatga agtaggttcc agtactaatg aagtgggctc
     7141 cagtattaat gaaataggtt ccagtgatga aaacattcaa gcagaactag gtagaaacag
     7201 agggccaaaa ttgaatgcta tgcttagatt aggggttttg caacctgagg tctataaaca
     7261 aagtcttcct ggaagtaatt gtaagcatcc tgaaataaaa aagcaagaat atgaagaagt
     7321 agttcagact gttaatacag atttctctcc atatctgatt tcagataact tagaacagcc
     7381 tatgggaagt agtcatgcat ctcaggtttg ttctgagaca cctgatgacc tgttagatga
     7441 tggtgaaata aaggaagata ctagttttgc tgaaaatgac attaaggaaa gttctgctgt
     7501 ttttagcaaa agcgtccaga aaggagagct tagcaggagt cctagccctt tcacccatac
     7561 acatttggct cagggttacc gaagaggggc caagaaatta gagtcctcag aagagaactt
     7621 atctagtgag gatgaagagc ttccctgctt ccaacacttg ttatttggta aagtaaacaa
     7681 tataccttct cagtctacta ggcatagcac cgttgctacc gagtgtctgt ctaagaacac
     7741 agaggagaat ttattatcat tgaagaatag cttaaatgac tgcagtaacc aggtaatatt
     7801 ggcaaaggca tctcaggaac atcaccttag tgaggaaaca aaatgttctg ctagcttgtt
     7861 ttcttcacag tgcagtgaat tggaagactt gactgcaaat acaaacaccc aggatccttt
     7921 cttgattggt tcttccaaac aaatgaggca tcagtctgaa agccagggag ttggtctgag
     7981 tgacaaggaa ttggtttcag atgatgaaga aagaggaacg ggcttggaag aaaataatca
     8041 agaagagcaa agcatggatt caaacttagg tgaagcagca tctgggtgtg agagtgaaac
     8101 aagcgtctct gaagactgct cagggctatc ctctcagagt gacattttaa ccactcagca
     8161 gagggatacc atgcaacata acctgataaa gctccagcag gaaatggctg aactagaagc
     8221 tgtgttagaa cagcatggga gccagccttc taacagctac ccttccatca taagtgactc
     8281 ttctgccctt gaggacctgc gaaatccaga acaaagcaca tcagaaaaag cagtattaac
     8341 ttcacagaaa agtagtgaat accctataag ccagaatcca gaaggccttt ctgctgacaa
     8401 gtttgaggtg tctgcagata gttctaccag taaaaataaa gaaccaggag tggaaaggtc
     8461 atccccttct aaatgcccat cattagatga taggtggtac atgcacagtt gctctgggag
     8521 tcttcagaat agaaactacc catctcaaga ggagctcatt aaggttgttg atgtggagga
     8581 gcaacagctg gaagagtctg ggccacacga tttgacggaa acatcttact tgccaaggca
     8641 agatctagag ggaacccctt acctggaatc tggaatcagc ctcttctctg atgaccctga
     8701 atctgatcct tctgaagaca gagccccaga gtcagctcgt gttggcaaca taccatcttc
     8761 aacctctgca ttgaaagttc cccaattgaa agttgcagaa tctgcccaga gtccagctgc
     8821 tgctcatact actgatactg ctgggtataa tgcaatggaa gaaagtgtga gcagggagaa
     8881 gccagaattg acagcttcaa cagaaagggt caacaaaaga atgtccatgg tggtgtctgg
     8941 cctgacccca gaagaattta tgctcgtgta caagtttgcc agaaaacacc acatcacttt
     9001 aactaatcta attactgaag agactactca tgttgttatg aaaacagatg ctgagtttgt
     9061 gtgtgaacgg acactgaaat attttctagg aattgcggga ggaaaatggg tagttagcta
     9121 tttctgggtg acccagtcta ttaaagaaag aaaaatgctg aatgagcatg attttgaagt
     9181 cagaggagat gtggtcaatg gaagaaacca ccaaggtcca aagcgagcaa gagaatccca
     9241 ggacagaaag atcttcaggg ggctagaaat ctgttgctat gggcccttca ccaacatgcc
     9301 cacagatcaa ctggaatgga tggtacagct gtgtggtgct tctgtggtga aggagctttc
     9361 atcattcacc cttggcacag gtgtccaccc aattgtggtt gtgcagccag atgcctggac
     9421 agaggacaat ggcttccatg caattgggca gatgtgtgag gcacctgtgg tgacccgaga
     9481 gtgggtgttg gacagtgtag cactctacca gtgccaggag ctggacacct acctgatacc
     9541 ccagatcccc cacagccact actagtaatg aactagtccg taacttgaaa gtatttcgat
     9601 ttcttggctt tatatatctt gtggaaagga cgaactgaac tgatcttggc gttatacacg
     9661 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    10021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    10081 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    10141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    10201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    10261 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
    10321 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
    10381 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10441 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    10501 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    10561 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    10621 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    10681 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10741 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    10801 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10861 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10921 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    10981 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    11041 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    11101 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    11161 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    11221 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    11281 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    11341 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    11401 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    11461 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    11521 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    11581 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    11641 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11701 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11761 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11821 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11881 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11941 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    12001 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    12061 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    12121 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    12181 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    12241 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    12301 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    12361 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    12421 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    12481 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    12541 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    12601 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    12661 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12721 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12781 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    12841 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12901 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12961 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    13021 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    13081 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    13141 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    13201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    13261 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    13321 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    13381 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    13441 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    13501 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    13561 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    13621 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    13681 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    13741 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    13801 agctggagct gcaagc
//
