LOCUS       sequence-404195-       12916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2777
FEATURES             Location/Qualifiers
     source          1..12916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4991..5002
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    8773..9361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8826..8846)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9244..9255)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9433..9666
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9744..9865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9781..9800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9835..9854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9905..10040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10060..10079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10061..10079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10089..10106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10089..10105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10098..10120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10247..10702
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10334..10353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10544..10565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10728..10832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10833..10901,10902..11693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11051..11070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11864..12452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12353..12372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12606..12623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12740..12761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12776..12781,12782..12799,12800..12806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12814..12830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12819..12841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12838..12854
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12838..12854
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12873..12893
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12875..12893
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag ccggggtccg acgatttcct
     4141 accgccaccg gagtgcccgg tgttcgagcc tagctgggcc gagttccgag accctcttgg
     4201 ctacatcgcg aaaatcaggc ccatcgcaga gaaatcgggc atttgcaaga tccgcccacc
     4261 cgcggactgg cagccaccct ttgctgtgga agtggacaac ttcaggttta ccccccgaat
     4321 ccagaggctg aatgagctag agattgtggt ggaggaaggt ggttatgaag ctatctgcaa
     4381 ggaccgtcgg tgggctcggg tagcccagcg cctcaactat ccaccaggca aaaatattgg
     4441 ctccttgcta cgctcccact acgaacgcat tgtttatccc tatgaaatgt accagtctgg
     4501 agccaacctt gtgcagtgta acacacgtcc atttgataat gaggagaagg acaaggaata
     4561 caaaccccac agcatccccc tacgacagtc tgtgcagcct tccaagttca acagctatgg
     4621 ccggcgggcc aagagactgc agcctgatcc ggaacccaca gaggaagaca ttgagaagaa
     4681 tccagagctg aaaaagctac agatctatgg ggcaggcccc aagatgatgg gcctgggcct
     4741 catggccaaa gacaagactc tgcggaagaa agataaggag gggcctgagt gtccccccac
     4801 agtagtggtg aaggaggagt taggtgggga tgtgaaggtg gagtcaacat cgcctaagac
     4861 cttcctggag agcaaggagg agctgagtca cagcccagaa ccctgcacca agatgaccat
     4921 gaggctacgg aggaaccaca gcaatgccca gtttattgag tcatatgtct gccggatgtg
     4981 ttctcgaggg gatgaggatg acaagctcct gctgtgtgat ggctgtgatg acaactacca
     5041 catcttctgc ctgctgcctc ctctgcctga gatccccaag ggtgtctggc ggtgcccaaa
     5101 gtgtgtcatg gcggagtgta agcggccccc agaagccttt ggctttgagc aggctacccg
     5161 ggaatacact ctgcagagct ttggcgagat ggccgactcc tttaaagctg actacttcaa
     5221 catgcccgtg catatggtgc ccacagaact tgtggagaag gagttctgga ggctggtaaa
     5281 tagcattgag gaagatgtga ctgttgagta tggagctgac atccattcca aagaatttgg
     5341 cagcggtttc cctgtcagtg acagtaaacg gcacctaacc cccgaagagg aggagtatgc
     5401 taccagtggt tggaacctaa atgtgatgcc ggtgttggaa cagtctgtac tgtgccacat
     5461 caatgcagat atctctggca tgaaggtgcc ctggctctac gtgggcatgg tcttctcagc
     5521 cttttgctgg catattgagg atcactggag ttactccatt aactacctcc actggggtga
     5581 gccgaagacc tggtatgggg tgccctcact tgcagcagaa catttggaag aagtgatgaa
     5641 gaagctgaca cctgaactat ttgatagcca gcctgacctc ctgcaccaac ttgtcaccct
     5701 catgaatccc aacaccctca tgtcccatgg tgtgccagtt gtccgcacaa accagtgtgc
     5761 aggagagttt gtcatcacct tcccccgtgc ttaccacagc ggcttcaacc aaggctacaa
     5821 ctttgccgag gctgtcaact tttgcactgc tgactggttg cctgctgggc gccagtgcat
     5881 tgagcactac cgccggctcc ggagatactg cgtcttctcc catgaggagc ttatctgcaa
     5941 gatggctgcc tgcccagaga agctagacct gaacctggcg gcagctgtgc ataaggagat
     6001 gttcatcatg gtgcaagaag agcggcgtct acgaaaggcc ctgctggaga agggtatcac
     6061 agaggctgag cgagaggctt tcgagctgct cccagatgat gagcgccagt gtatcaagtg
     6121 caagactacg tgtttcctgt cagccctggc ctgctacgac tgcccagacg gccttgtctg
     6181 cctttcccac atcaatgatc tctgcaagtg ctccagtagc cggcagtacc tgcggtatcg
     6241 gtataccttg gatgagcttc ctgccatgct gcataagctg aaggttcggg ctgagtcctt
     6301 tgacacctgg gccaacaaag tgcgagtggc cctggaggtg gaggatgggc ggaagcgcag
     6361 ccttgaagaa ctgagggcac tagagtctga agcccgtgag cggaggtttc ctaatagtga
     6421 gctgctgcag caactaaaga actgcctgag tgaggcagag gcttgcgtgt cccgagctct
     6481 gggactggtc agcggccagg aagctggccc ccacagggtg gctggtctac agatgaccct
     6541 gactgagctc cgggcctttc tggaccagat gaacaacctg ccttgcgcca tgcaccagat
     6601 tggggatgtc aagggtgttc tggaacaggt ggaggcctac caggctgagg ctcgtgaggc
     6661 cctggcctca ctgccctcca gtccagggct actgcagtcc ctgttggaga gggggcggca
     6721 gctgggggtg gaggtgcctg aggcccagca gctccagcgg caggtggaac aggcgcgatg
     6781 gctggatgag gtgaaacgca cactggcccc ctcagcccga aggggcacct tggctgtcat
     6841 gcgaggactg ttggtcgcgg gtgccagtgt agcccctagc cctgctgtgg ataaagccca
     6901 ggccgagctg caggaactgc tgaccattgc tgaacgctgg gaggagaaag cccacctctg
     6961 cctggaggcc aggcagaagc atccaccagc cacacttgag gccataatcc gtgaagcgga
     7021 aaacatccct gttcacctgc ccaacatcca ggctctcaag gaggctcttg ctaaggcccg
     7081 ggcctggatt gctgatgttg atgagatcca aaatggtgac cactacccct gcctggatga
     7141 cttggagggc ctagtagctg tgggccggga cctacctgtg gggctggagg agctgagaca
     7201 gctagagcta caggtactga cagcgcactc ctggagggag aaggcctcca agaccttcct
     7261 caagaaaaat tcttgctaca cgctgctgga ggttctctgc ccatgtgcag atgccggctc
     7321 agacagcacc aagcgcagcc ggtggatgga gaaggagctg gggttgtaca aatctgacac
     7381 agagctgctg gggctgtctg cgcaggacct cagggaccca ggctctgtga tcgtggcctt
     7441 caaggagggg gaacagaagg agaaggaggg tatcctgcag ctgcgtcgca ccaattcggc
     7501 caagcccagt ccactggcat catcgagcac ggcctcctct acaacctcta tctgtgtgtg
     7561 tgggcaggtg ctggctgggg cgggagctct gcagtgtgac ctgtgtcagg actggttcca
     7621 tgggcggtgt gtgtcagtgc ctcgcctcct cagctctccg aggcccaatc ccacctcatc
     7681 cccactgctg gcctggtggg aatgggacac caaattcctg tgtccactgt gtatgcgctc
     7741 aaggcgcccg cgcctggaga ccatcctggc actgctggta gccctgcaga gactgcctgt
     7801 gcggctgccc gagggcgagg ccctgcagtg cctcacagag agggccatca gctggcaagg
     7861 ccgcgccagg caggctctgg cctctgaaga tgtgactgct cttttgggac ggctggctga
     7921 gctccgccaa cggctacagg ctgaacctag acctgaggag cctcctaact accctgcagc
     7981 ccctgcttct gaccccctca gagagggcag tggcaaggat atgcctaagg tccagggctt
     8041 actggagaat ggagacagtg tgaccagtcc tgagaaggta gccccggagg agggctcaga
     8101 tctggagctg ctgtcctcgc tgttgccaca gttgactggc cctgtgttgg aactgcctga
     8161 ggcaacccgg gcccccttgg aggagctcat gatggagggg gacctgctcg aggtgaccct
     8221 ggatgagaac cacagcatat ggcagctgct gcaggctgga cagcccccag acctggagag
     8281 gatccgcaca cttctggagc tggagaaggc agagcgtcac gggagtcggg ctcggggccg
     8341 ggccctggag aggcggcggc ggcggaaggt ggatcggggt ggggagggcg atgacccagc
     8401 ccgagaggag ctagagccaa agagggtacg gagctcaggg ccagaggctg aggaggtcca
     8461 ggaggaggaa gagctggagg aggagactgg gggtgagggc ccccctgcac ccatccccac
     8521 cactggcagc cccagcaccc aggagaacca gaatggcttg gaaccggcgg aagggaccac
     8581 ttcaggcccc tcggcccctt tctccactct gactccccgg ctgcatctgc cctgcccaca
     8641 gcagccgcct cagcaacagt tgtagtaatg aactagtccg taacttgaaa gtatttcgat
     8701 ttcttggctt tatatatctt gtggaaagga cgaggccaat aacaaccaca gaagtccacg
     8761 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8821 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8881 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8941 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9001 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9061 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9121 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9181 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9241 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9301 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9361 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9421 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9661 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9721 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     9781 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9841 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     9901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10021 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    10081 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    10141 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    10201 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    10261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    10321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10681 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12661 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    12721 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    12781 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    12841 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    12901 agctggagct gcaagc
//
