LOCUS       sequence-404198-       16315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16315)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2746
FEATURES             Location/Qualifiers
     source          1..16315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12172..12760
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12225..12245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12643..12654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12832..13065
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13143..13264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13180..13199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13234..13253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13304..13439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13459..13478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13460..13478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13488..13505)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13488..13504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13497..13519)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13646..14101
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13733..13752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13943..13964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14127..14231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14232..14300,14301..15092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14450..14469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15263..15851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15752..15771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16005..16022
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16139..16160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16175..16180,16181..16198,16199..16205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16213..16229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16218..16240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16237..16253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16237..16253
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16272..16292
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16274..16292
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggct gtgtgggagg ccgagcagct
     4141 gggtggcctc cagcgggggg acctgctcac accccctgcc cctgatggcg acgggcgcac
     4201 ggcccccctt ggccagcccc ctggggccca gctgtactgc ccggcctgct tggtcacctg
     4261 ccactctcag gaggccttcg agaaccactg cgcatcctcg gagcacgcac agatggtggc
     4321 cttcgaccag gccctgccct gggagcaccg ttccccaccc ccgggactct ccaagttcga
     4381 gctctgccca aagcctgacc tctgtgagta tggggacgcc tgcaccaagg cacactcagc
     4441 acaggagctg caggagtggg tccggcgcac gcaggctgtg gagctgcggg ggcaggcggc
     4501 ctggcaggac gggctggtgc cctaccagga gcggctgctg gccgagtacc agcgcagcag
     4561 cagtgaggtc cttgtgctgg cagagaccct tgatggagtg cgtgtcacct gcaaccagcc
     4621 cctgatgtac caggcccagg agaggaagac ccagtacagc tggacgtttg ccgtccactc
     4681 tgaggagccc ctgctacacg tggccctgct gaagcaggag ccaggagccg acttctctct
     4741 ggtggctccc ggcctcccgc caggccggct ctacgcacgg ggtgagcgct tccgtgtgcc
     4801 cagctccact gccgacttcc aggtgggagt gcgtgtgcag gctgcctcgt tcggcacctt
     4861 tgagcaatgg gtggtcttcg actttggccg ccggccggtg ctgctacaaa agctggggct
     4921 gcagctgggc caggggcgtc gcccaggacc ctgcaggaat ctggcgctcg gccaccctga
     4981 ggagatggag cgctggcaca ctggcaaccg ccacgtggtg cctggcgtgg agcggacggc
     5041 cgagcagacg gccctgatgg ccaagtacaa gggccctgcc ctggccctgg agttcaaccg
     5101 cagcagcgtg gcctcgggcc ccatctcacc aaccaactat cggcagagga tgcaccagtt
     5161 tctctatgag gaggaggcgg ctcagcagca gctggtggcc aagctgaccc tgcggggcca
     5221 ggtgttcctg aagacggcat tgcagacgcc agcgctgaac atgctcttcg cgcctccggg
     5281 agcactgtac gcagaggtcc ccgtcccctc ctccctgatg ccagacacag accagggctt
     5341 cctgctgggc cgggcggtca gcacagccct ggtggcccct gtacctgcac ccgacaatac
     5401 ggtgttcgag gtgcggctgg agaggcgggc cagctcagag caggcgctgt ggctgctgct
     5461 tccggcccgc tgctgcttgg ccctggggct gcagcctgag gcccgcctgg tcctggaggt
     5521 gcagttccag attgacccga tgaccttccg cctctggcac caggcagtgg acacactgcc
     5581 tgaggagcag ctggtggtgc ccgacttgcc cacctgcgcc ctgcccagac cttggtctgt
     5641 cccacccttg cggcgtggca accgcaagca ggagctggcc gtggcgctca tcgcgggctg
     5701 gggccctggg gatgggaggc gtgtcccccc gctactcatc tatggcccct ttggcaccgg
     5761 caagacctac acgctggcca tggcctccct ggaggtcatc cggaggcctg aaaccaaggt
     5821 gctcatctgc acacacacca acagtgccgc cgacatctac atccgggagt atttccacag
     5881 ccacgtcagc ggcggccacc ccgaggccac tcctctccgt gtgatgtaca cggaccggcc
     5941 gctgagccag acggacccag tcacgctgca gtactgttgc ctgaccgacg accgccaggc
     6001 tttccgcccg cccacacggg cagagctggc gcggcaccgc gtggtggtca ccaccacctc
     6061 ccaggcccgt gagctcaggg tgccggtcgg cttcttctcc cacattctca tcgatgaggc
     6121 ggcccagatg ctggagtgcg aggccctcac cccgctggcc tatgcctcgc acggcacccg
     6181 cctcgtgctg gcgggcgacc acatgcaggt cacaccccgg ctgttcagtg tggccagggc
     6241 ccgggcggcc gagcacacgc tgctgcaccg cctcttcctg tgctaccagc aggagactca
     6301 cgaggtggcg cggcagagcc gcctggtctt ccacgagaac taccgctgca cggacgccat
     6361 tgtcagcttc atctcgcggc acttctacgt ggccaagggc aaccccatcc acgccagggg
     6421 caaggttccg ccccaccccc ggcactaccc gctcatgttc tgccacgtgg cgggcagccc
     6481 agaccgggac atgtccatgg cgtcctggct gaatctggct gagattgcgc aggtcgtcga
     6541 gaaggtgcag gaggcctaca acacctggcc cagctgctgg ggcggccgcg agcagaggtg
     6601 catctgtgtc gtttcccacg gtgcccaggt cagtgcactg aggcaggagc tgaggaggcg
     6661 ggacctaggc caggtgtctg tcggcagttt tgagatcctg ccagggcggc agttccgggt
     6721 cgtggtgctc agcactgtgc acacctgcca gagcctgctc agccctgggg cactggcccc
     6781 tgagttcttc accgacgccc gcgtgctcaa caccgtcctg acccgcgccc agtcccagct
     6841 ggtggtagtg ggggacgccg tggccctctg ctccttcggg gcctgcggca agctctggga
     6901 gagcttcatc cgtgagtgcg tggagcggca cagtgtctgc cccgagggcc tgtccatgga
     6961 gcaggtcgag cagggtgtgg cgcagagacg gcgctggcct ccccgaggca cacaggctgg
     7021 ggcagcgggg aactgggagg ctgccccaga gccagtaggg gacctggccg aggagcaggc
     7081 ggctgtggtg acggccatgg tgaaggcaga gccgggagat gaggctctga gcccagcatc
     7141 ccgtgacatc acggcaacca cagcgcagac ggaggctgcg gcagcaccag caggagacgc
     7201 agtgaaggaa gacgtggtgc ccggggcctg tgcggcagga gcggctgctg cagcgggcgt
     7261 ggagtccacg gaggctgagg atgcagaggc tgacttctgg ccgtgggacg gggagctcaa
     7321 cgctgacgac gccatcctac gggagcttct ggacgagagc cagaaggtga tggtgaccgt
     7381 cggggaggac gggctgctgg acactgtcgc caggcccgag tccctgcagc aggcccggct
     7441 gtacgagaac ctgcccccgg ctgcgctacg gaagctgctg cacgcggagc ccgagcggta
     7501 ccgccactgc tctttcgtgc cagagacctt cgagcgggcg tcagccatcc cgctggacga
     7561 tgcctcctcg ggccccatcc aggtcagggg ccgcctggac tgtgggatgg ccttcgccgg
     7621 ggatgaggtg ctggtgcagc tcctttcggg agacaaggcg cccgaggggc ggcttcgggg
     7681 ccgcgtgctg ggcgtgctga agaggaagag gcacgagctg gcgtttgtgt gccgcatgga
     7741 cacgtgggac ccgcgcatca tggtccccat caatggctcc gtgaccaaga tcttcgtggc
     7801 cgagctgaag gacccatcgc aggtccccat ctacagcctc cggaagggcc ggctgcagcg
     7861 tgtggggctt gagaggctca ccgccgaggc ccggcacagc cggctcttct gggtccaaat
     7921 tgtcctgtgg cggcaaggct tctactaccc gctgggcatc gtccgggagg tgctgcctga
     7981 ggccagcacc tgggagcagg gcctccgcat cctcggcctg gagtacagct tgagggtgcc
     8041 cccgtcggac caggccacca tcaccaaggt gctgcagaaa taccacacgg agcttggccg
     8101 ggttgccggc cgccgagagg actgccgcgc cttcttgacc ttcactgtgg acccccaggg
     8161 cgcctgcaac ctcgatgatg ccctcagtgt ccgagacctg ggtcccaggt gcgaggtggc
     8221 tgtgcacatc actgatgtgg ccagcttcgt gcccagggac ggggtgctgg acgtggaggc
     8281 gcgaaggcag ggcgctgcgt tctatgcccc cggcagggag ccagtgccca tgctgccggc
     8341 cagcctctgc caggacgtcc tcagcctcct gcctggccgg gaccgcctgg ccatctccct
     8401 gttcctcacc atggagaagg ccagtggcca gctgaagagc ctgcgctttg caccctccgt
     8461 ggtccagtct gaccgccagc tgtcctacga ggaggcggag gaggtgatca ggcagcaccc
     8521 gggtgccggc cgtgagctgc cggcccgcct ggactccgtg gacgcctgcg tcgtggccgc
     8581 gtgctacttc tctcggctgc tgcgccggca ccgcctgcgg tccgactgct tctatgagca
     8641 gccggacgag gacggcaccc tgggcttccg cgcggcccac atcatggtga aggagtacat
     8701 gattcagttt aataggctcg tggctgagtt cctggtgggc agcgagtgca cgcggacggt
     8761 cacgcctctg cggtggcagc cagcaccccg cagccagcag ctcaaggccc tgtgtgagaa
     8821 gcatggggac cgggtgcccc tgtcactgca cctcggccac cacctgcacg gcggcggggg
     8881 cagtcccccc gacacgcggc tgcacctcct ggcctccctc tggaagcagg tccagtttgc
     8941 tgcccgcacc caggactacg aacagatggt ggacttggtc accacggacg acatgcaccc
     9001 attcctggct cctgcaggcc gcgacctccg caaggccctg gagcgctcgg cgttcggccg
     9061 ctgcgcccgg ggccaccagc agcagggcgg ccactactcg ctgcaggtgg actggtacac
     9121 gtgggccacc tcgcccatcc gcaggtacct ggacgtggtg ttgcagcggc agatcctgct
     9181 ggcgctgggc catgggggct ctgcctactc tgccagggac atcgatgggc tctgccaggc
     9241 cttcagcctc cagcacgcac ttgcccagag ctatcagcgg cgggcgcgca gcctgcacct
     9301 ggccgtgcag ctcaaggccc agcctctgga caagctgggc ttcgtggtgg acgtggaggc
     9361 gggctcccgc tgcttccggc tgctcttccc cagcaaccgg gagacgctgc ctgacccctg
     9421 ccccgtcccc tacggctccc tgcagctggc cgagcacccc cacgccctgg caggccggcc
     9481 gggcctgcgg ctcctgtggc ggcgccgtgt ctactcagcg cagggatcca gcccgcccct
     9541 gccactgcct ggcactgtgc cggacccaca caccctggcc gtggagacgg ccctgtggaa
     9601 gcagctgctg gagctggtgg agctgcagcg ctggccggag gcggctgctc tcatccagga
     9661 gaagggcgag gcgtcccagc ggcgggagct ggtgcaggtg cagcggagcc actgtggcca
     9721 tttcctggag gtggcccggg agctgggcag tggggacacc ctgcaggtgc agctcggcac
     9781 cagcctgcag cacggcttcc tggtaccgag ccctcagctc tggacggtgg caccgggctt
     9841 cagcctctgc ctggagcacg tggagcggcc cggagactgc ttctcaggcc gtgtgtaccg
     9901 ggccccgagg gaccggtacc gcgacgtgga tgagtacgcc tgcgtgtggg aaccattctg
     9961 cgccctggag tcggccaccg gcgcggttgc cgagaatgac tccgtcacac ttcagcacct
    10021 gagtgtctcc tgggaggcgt cacggacgcc gcaggggcag ctgcagggcg ccttccgcct
    10081 ggaggccgcc ttcctcgagg agaactgtgc cgacatcaac ttcagctgct gctacctctg
    10141 catccggctc gaggggctgc cggctcccac ggccagccca cgccctgggc ccagcagcct
    10201 cggccctggc ctgaatgttg accccggcac gtatacctgg gtggcccacg ggcagacgca
    10261 ggactgggac caggagcgcc gggcagaccg gcaggaggct cccagacggg tgcacctctt
    10321 cgtccaccac atgggcatgg agaaggttcc ggaagaggtg ctgaggccgg gcaccctgtt
    10381 caccgttgag ctgctgccca agcagcttcc tgatctccgc aaggaggaag ccgtgcgtgg
    10441 actagaggag gcgtccccgc tggtcaccag catcgcactg ggccggcctg tcccgcagcc
    10501 cctctgcaga gtgatcccca gcaggttcct ggagcggcag acctacaaca tccccggagg
    10561 ccgccacaag ctgaacccca gccagaacgt ggcggtcagg gaggctctgg agaagccttt
    10621 cacggtcatt cagggcccac caggtacagg gaagacgatc gtgggcctcc acatcgtatt
    10681 ctggtttcat aaatcaaacc aggagcaggt gcagcccgga ggcccccccc gtggggagaa
    10741 gcggctgggg ggtccctgca tcttgtactg cggcccctcc aacaagtcgg tggatgtcct
    10801 ggcaggactg ctcctgagaa ggatggagct gaagcccctc cgtgtgtaca gtgagcaggc
    10861 tgaggccagc gagttcccag tgccgcgtgt gggcagcagg aagctgctca ggaagagccc
    10921 ccgggagggg aggccgaacc agagcctcag gagcatcacc ctgcaccacc ggatccggca
    10981 ggcccccaac ccttactcgt cggaaatcaa ggcctttgac acccggctgc agagagggga
    11041 gctcttctcc agggaggacc tggtctggta caagaaggtc ttgtgggagg ctcggaagtt
    11101 cgagctggac cggcatgagg tcatcctctg cacctgctcc tgtgcagcct ctgccagcct
    11161 caaaatcctg gacgtgaggc agatccttgt tgacgaggca ggcatggcca cggaacctga
    11221 aaccctcatc cccctggtgc agttcccaca ggccgagaag gtggttcttc tcggagacca
    11281 caagcagctg cggcctgtgg tcaagaatga gcggctgcaa aacctgggtc tggaccggtc
    11341 tctgttcgag cggtaccacg aggacgcaca tatgctggac actcagtacc gcatgcatga
    11401 gggcatctgt gccttcccct ctgtggcgtt ctacaagagc aagctgaaga cgtggcaggg
    11461 cctgaggagg ccgcccagtg tcctgggcca cgctggcaag gagagctgcc ctgtcatctt
    11521 tggccacgtg cagggccacg agcggagcct gctggtgtcc acggacgaag ggaatgagaa
    11581 ctccaaggcc aacctggagg aggtggctga ggtggtccgt atcaccaagc agctgaccct
    11641 ggggaggacc gtagagcccc aggacatcgc cgtcctcacg ccctacaacg cgcaggcctc
    11701 tgagatcagc aaggcccttc ggcgagaggg catcgccggg gtggccgtgt cctccatcac
    11761 caagagccag gggagcgagt ggcgctatgt gctggtgagc accgtccgca cctgtgccaa
    11821 gagcgacctg gaccagcggc ccaccaagag ctggctcaag aagtttctgg gcttcgttgt
    11881 ggaccccaac caagtgaatg tggctgtcac gcgggcccag gaggggctct gcctgatcgg
    11941 agaccacctc cttctgcgct gctgccccct ctggcgtagc ctcctggact tctgcgaggc
    12001 tcagcagacc ctcgtgcctg ccggccaggt gcgcgtctgc aggaggccaa ctatgccttc
    12061 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
    12121 tggaaaggac gaatatgaac gtatggcata gcgtgaacgc gttaagtcga caatcaacct
    12181 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    12241 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    12301 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    12361 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    12421 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    12481 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    12541 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    12601 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    12661 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    12721 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
    12781 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    12841 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    12901 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    12961 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    13021 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
    13081 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    13141 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    13201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    13261 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    13321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    13381 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    13441 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    13501 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    13561 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    13621 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    13681 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    13741 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    13801 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    13861 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    13921 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    13981 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    14041 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    14101 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    14161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    14221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    14281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    14341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    14401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    14461 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    14521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    14581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    14641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    14701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    14761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    14821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    14881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    14941 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    15001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    15061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    15121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    15181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    15241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    15301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    15361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    15421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    15481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    15541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    15601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    15661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    15721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    15781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    15841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    15901 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    15961 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    16021 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    16081 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    16141 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    16201 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    16261 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
