LOCUS       sequence-404199-       12031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2400
FEATURES             Location/Qualifiers
     source          1..12031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7888..8476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7941..7961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8359..8370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8548..8781
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8859..8980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8896..8915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8950..8969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9020..9155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9175..9194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9176..9194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9204..9221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9204..9220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9213..9235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9362..9817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9449..9468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9659..9680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9843..9947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9948..10016,10017..10808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10166..10185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10979..11567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11468..11487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11721..11738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11855..11876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11891..11896,11897..11914,11915..11921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11929..11945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11934..11956
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11953..11969
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11953..11969
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11988..12008
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11990..12008
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcta cgctccactg gcttcttccg
     4141 ggccattgac tgcccctatt ggtctggggc gcccgggggg ccctgccggc ggccctactg
     4201 ccacttccgg caccgcgggg cccggggctc cggcgcgccc ggtgacggcg gagaggcgcc
     4261 ccccgcagca gggctgggtt acgaccccta caaccctgag ctgcccaagc cccccgcgca
     4321 gagggagaat ggcaccctgg gcctggggga ggagccgcgc ccggatgtgc tggagttgga
     4381 gctggtcaac caggccatcg aggccgtgcg cagtgaggtg gagctggagc agcggcgcta
     4441 ccgggagctg ctggagacga cccgtgagca ccgctccgcc gaggcccccg ccctggcgcc
     4501 ccgcggcccc aacgccagcc ccactgtggg cccggacgag gatgccttcc cactggcctt
     4561 cgactacagc cccggcagcc acggcctatt aagccctgat gccggctacc agcccacccc
     4621 actggccgcc cctgccgagc cgggcagcaa gtactcgctg gcgtccctgg acaggggtca
     4681 gggcagaggt ggagggggtg gcggtgccct ggaatacgtc cccaaggctg tgagccagcc
     4741 ccggcggcac agccgccccg ttcccagtgg caagtacgtg gtggacaact ccaggccacc
     4801 cacagacctg gagtatgacc ctctctccaa ctactcggcc cggcacctca gcagggccag
     4861 ctcccgggat gagcgggccg ccaagcggcc ccggggctcc cgcggcagtg agccctacac
     4921 acctgctccc aagaagctct gtgacccctt tggcagttgc gatgcaaggt tctcagactc
     4981 agaagatgag gccgccacgg tcccaggtaa cgagcccacc acggccagca cccccaaagc
     5041 cagggccgac cctgagatca aggccaccgg gcagccaccc tccaaagagg gcctggaggc
     5101 cgaggggggc ggcctgcggg agaccaagga gacggccgtg cagtgcgacg tgggggacct
     5161 ccagccgccc ccagccaagc ccgcctcccc agcccaggtc cagtcctcac aggatggggg
     5221 ctgccccaag gagggaaaac ccaagaagaa aaaaaccggg gccccacctg cccccagctg
     5281 caaagacggg gcccagggga aggacaagac caaggacaag ggccgagggc ggcctgtgga
     5341 gaagccccgt gcggacaaga agggcccgca ggccagcagc ccccggcgca aggcagagcg
     5401 gccggaaggg accaagaaga agccatcttc ggccactcct gtggccacct cagggaaagg
     5461 gaggcctgac cggccagcgc ggcggccgag ccccacaagc ggggactccc gaccggcggc
     5521 cggcagaggc ccaccccgcc ccctccagct gcccgacagg aagagcacca aggccccgtc
     5581 ggggaagcta gtggagcgga aagcccgctc actagacgag ggcgcctccc aggacgcccc
     5641 caagctgaag aagcgggccc tgagccacgc cgacctcttt ggggacgaga gtgaggacga
     5701 ggccgcaggg ccaggggtgc cgagcgtgtg gccctctgcc ctccccagcc tcagctcgga
     5761 ctcagactcc gactcagact ccagcctggg cttcccggag gcgcaggggc cgcccaagcg
     5821 gctcaaggcc tccccgcccc cctcccccgc cccatcctcc tcctcctcct cctcctcctc
     5881 cacctccagc gcgggggcgg atgtggacta ctcggccctg gagaaggagg tggactttga
     5941 ctccgacccc atggaggagt gcctgcggat cttcaacgag tccaccagcg tcaagacgga
     6001 ggacagaggc cggctggccc ggcagccccc caaggaagag aagagtgagg agaaggggct
     6061 ttcgggtctg accactctgt tccccgggca gaagaggagg atctcccacc tttccaagca
     6121 aggccaggag gtggagcccc cgaggagggg tcccgcggtg cccccggccc ggcccccgac
     6181 ggcgcaggag gtgtgctacc tgcgggccca gcaggcgcag agggcatcgg cgagcttgct
     6241 gcaggccccc gccaggctgg cagagaagtc gccctccgtc cacatttccg cccctggcga
     6301 gaagaggagg atcgcccaca tccccaaccc ccgcctggct gcagccccca caggtgccaa
     6361 gaggaccctt gcggccagcg gcagccagtc ctccaacggc cctgagccag gtggccagca
     6421 gctgaaaaca cgcacattgt cggggatggc gtccaagact accaccacca tcatccctaa
     6481 gcgaatcgcc cacagtccat ccttacagag tttaaagaaa cccattatcc ccaaagagtt
     6541 tgggggcaaa gtccccaccg tcatccgcca gcgctatctc aacctgttca tcgaggagtg
     6601 tctcaagttc tgtacctcca accaggaggc catagagaag gcactgaacg aggagaaggt
     6661 ggcctatgac cgcagcccca gcaagaacat ctacctgaat gtggccgtga acaccctcaa
     6721 gaagctcagg ggcctggccc ccagcgctgt gcccggcctc agcaaaacca gtggccgcag
     6781 ggttgtgtcc cacgaggtgg tgttgggggg caggttggcc gccaagacca gcttctcgct
     6841 cagccgtcca agcagccccc gggtggagga cctgaaaggg gctgccctgt acagccgcct
     6901 cagggagtac ctgctcaccc aggaccagct caaggagaac ggctacccct tcccgcaccc
     6961 agagcggccc gggggcgcaa tcatcttcac agctgaggag aagaggccca aggactcttc
     7021 ctgcaggacc tgctgccgct gtggcaccga gtacctcgtg tcctcttcag gccgctgcat
     7081 ccgggacgag gagtgttatt accactgggg acggctgcgc cggaaccggg tggccggagg
     7141 ctgggagacc cagtacatgt gctgctcggc tgccgccggc tctgtcggct gccaagtcgc
     7201 aaagcaacac gtgcaggatg gccggaagga gcgccttgag ggcttcgtga agacctttga
     7261 gaaagagctc tcaggagaca cccacccggg gatctacgcc ctggactgcg agatgtccta
     7321 caccacatat ggcctggagc tgacgcgcgt cacggtggtc gacacggacg tgcacgtggt
     7381 ttatgacacc ttcgtgaagc ctgacaacga gatcgtggac tacaacacca ggttttcggg
     7441 ggtgacggag gctgaccttg ccgacacaag tgtcacgctg cgtgacgtcc aggccgttct
     7501 gctgagcatg ttcagcgctg acaccatcct catcggacac agcctggaga gcgacctcct
     7561 ggccctgaag gtcatccaca gcaccgtggt ggacacgtct gtgctcttcc cccaccgcct
     7621 gggcctcccc tacaagcggt ccctgcggaa cctcatggcc gactacctca gacagatcat
     7681 ccaggacaat gtggatgggc acagctccag cgaggacgcc ggcgcctgca tgcacctggt
     7741 gatctggaag gttcgagaag acgccaagac caagcgatag taatgaacta gtccgtaact
     7801 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagt ggagctatat
     7861 catctgataa gtacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8281 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8401 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8461 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8521 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8581 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8641 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8701 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8761 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     8821 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8881 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8941 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9121 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9181 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9361 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9421 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9481 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9541 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9601 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9661 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9721 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9781 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     9841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11641 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11701 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11761 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    11821 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    11881 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    11941 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12001 actaaaggga acaaaagctg gagctgcaag c
//
