LOCUS       sequence-404273-       14557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2116
FEATURES             Location/Qualifiers
     source          1..14557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(8607..8618)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    10414..11002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10467..10487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10885..10896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11074..11307
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11385..11506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11422..11441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11476..11495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11546..11681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11701..11720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11702..11720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11730..11747)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11730..11746)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11739..11761)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11888..12343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11975..11994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12185..12206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12369..12473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12474..12542,12543..13334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12692..12711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13505..14093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13994..14013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14247..14264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14381..14402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14417..14422,14423..14440,14441..14447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14455..14471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14460..14482
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14479..14495
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14479..14495
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14514..14534
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14516..14534
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtt ttggatgacc ttccaaactt
     4141 agaagacatc tatacttcct tgtgttcatc aacaatggaa gactcagaga tggattttga
     4201 ctctggacta gaagatgatg acacaaaaag tgatagtatt ttggaggatt ccacaatttt
     4261 tgtggccttc aaaggaaata tagatgataa agacttcaaa tggaaattag atgcaatatt
     4321 gaaaaacgtg cccaatttgt tacacatgga gtccagcaag ctaaaagtac agaaggtgga
     4381 gccctggaac agcgtgcgtg tgacattcaa catcccccgg gaagcagcgg agcggctacg
     4441 gatccttgct cagagcaaca accagcagct tcgggattta gggattctct ccgttcagat
     4501 tgaaggggaa ggtgctatta acctggcttt ggctcagaac cgaagccaag atgtgagaat
     4561 gaatggaccc atgggagctg gaaattcagt taggatggag gcgggatttc ctatggcaag
     4621 tggtccagga ataataagga tgaacaaccc tgccactgtt atgatacccc cgggtggaaa
     4681 tgtgtcatct tccatgatgg caccaggccc caatccagag ctgcagccca ggactcctcg
     4741 ccctgcttct cagtcagatg caatggatcc actcctctct gggctccata tacagcagca
     4801 aagtcatccc tcaggatctt tagctccccc acatcaccca atgcagcctg tctctgtgaa
     4861 cagacaaatg aacccagcta attttcccca gctgcagcag cagcagcaac aacaacaaca
     4921 gcagcagcag cagcagcagc agcaacaaca gcaacagcag caacaacagt tgcaggcaag
     4981 acccccacag caacatcagc agcaacagcc acagggaatt cgaccccagt ttactgcccc
     5041 aactcaggtg cctgttcctc caggctggaa ccagctgcct tctggagccc ttcaacctcc
     5101 tccagcccag ggttctctgg gcacaatgac tgcaaaccaa gggtggaaga aggctccctt
     5161 gcccggccca atgcaacagc aactccaggc aagaccatcc ttagccacgg tacagacgcc
     5221 ttcccaccct ccccctccat atccctttgg cagccagcaa gcctcacaag cccacacaaa
     5281 ctttcctcag atgagcaacc caggccagtt cacagctcct cagatgaaga gtttgcaggg
     5341 agggccctct agggtcccaa ctcccttgca gcagccccac ctcaccaaca agtctcctgc
     5401 ctcctcaccc tcctccttcc agcagggatc ccctgcatcc tccccaacgg ttaaccaaac
     5461 tcagcagcag atgggaccaa ggccacctca aaataaccca cttccccagg gatttcagca
     5521 gcctgtcagc tctccgggtc ggaatcctat ggttcaacag ggaaatgtgc cacctaactt
     5581 catggtgatg cagcagcaac caccaaacca ggggccacag agtttacatc caggcctagg
     5641 aggaatgcct aaacgcctcc cacctggctt ctcagcagga caggccaatc cgaactttat
     5701 gcaaggtcag gtgccttcga ccacagcaac cacccctggg aattcaggag cccctcagct
     5761 gcaagcaaat caaaatgtcc agcatgcagg tggtcaagga gctggtcctc ctcaaaacca
     5821 gatgcaggtg tcccacgggc cgccaaatat gatgcagccc agcctcatgg gaattcatgg
     5881 caacatgaac aatcagcagg ctggtacttc tggggttcct caagtgaacc tcagcaacat
     5941 gcaaggccag ccccagcagg gcccaccatc tcagctgatg ggcatgcacc agcaaatcgt
     6001 gccctcccag ggccagatgg tccagcaaca aggaaccttg aaccctcaga accctatgat
     6061 cctttcaagg gcccagctta tgccacaggg ccagatgatg gtgaaccccc cgagccaaaa
     6121 tcttgggccc tcgccccaaa ggatgacccc acccaagcag atgctttccc agcagggccc
     6181 acaaatgatg gcgccacata accagatgat ggggcctcag gggcaggttt tgctccaaca
     6241 gaacccaatg atagagcaga ttatgaccaa tcaaatgcag gggaataagc agcagtttaa
     6301 cactcagaac cagtccaatg tcatgccggg accagcccag ataatgaggg gaccaactcc
     6361 aaacatgcaa ggaaatatgg tgcagtttac gggacagatg tcaggacaga tgctgcccca
     6421 gcaagggcct gtgaacaaca gtccatctca ggttatgggc attcagggac aggtcctgcg
     6481 gccaccaggg cccagcccac acatggccca gcagcatggt gatcctgcta ctacagcaaa
     6541 taacgatgtc agtttatctc agatgatgcc tgatgttagc attcaacaaa ccaacatggt
     6601 cccccctcat gtgcaggcca tgcagggaaa cagtgcctcg ggaaaccact tctcaggcca
     6661 tgggatgtct ttcaatgcac ctttcagtgg agctcccaat ggaaatcaga tgtcctgtgg
     6721 tcaaaatcca ggcttcccag tcaataagga tgtcacgcta acgagcccat tgttggtcaa
     6781 cttattgcag agtgacatat ctgcaggcca ttttggggta aacaataagc aaaataatac
     6841 caacgcaaat aaaccgaaga agaagaaacc ccctcggaag aagaaaaata gtcagcaaga
     6901 tctaaacacc ccagatactc gcccagctgg tctggaagag gctgatcagc caccgttgcc
     6961 tggagaacaa ggaattaact tggataactc aggccctaaa ctgccagaat tttcaaaccg
     7021 gccaccaggt tatccttctc aaccagttga acagaggcca cttcagcaga tgcctcctca
     7081 actcatgcag catgtggcac ccccaccaca gccaccacag cagcagccac agccacaact
     7141 gcctcagcag cagcagccac cacctcccag tcagccacag tctcagcagc agcagcagca
     7201 gcagcaacaa atgatgatga tgctcatgat gcagcaggat cccaaatcag ttaggcttcc
     7261 agtctctcaa aatgtccatc ctccaagggg ccccctgaac cccgactccc agagaatgcc
     7321 catgcaacag agtggcagtg tgcctgtcat ggtcagtctg caaggacctg cctccgtgcc
     7381 accatcacct gataaacaaa gaatgccaat gcctgtgaat actcccttgg gaagcaattc
     7441 aaggaaaatg gtctatcagg agagcccgca gaatccttcc agctcgccac tggcggagat
     7501 ggcctcactc cctgaagcaa gtggcagtga agcaccatct gtcccaggag gcccaaacaa
     7561 catgccttca catgtagtac ttccccagaa tcagttaatg atgacagggc caaaacctgg
     7621 accatcgccc ctttcagcaa ctcaaggtgc aactccccag caaccccctg taaattccct
     7681 gcccagctct cacggccacc acttcccaaa tgtggctgcg ccaacccaga catctaggcc
     7741 caaaacacca aacagagcca gccccagacc ctattatcct cagacaccca acaaccgccc
     7801 tcccagcaca gaaccttcag aaatcagtct gtcaccagaa agactcaatg cctccatagc
     7861 aggactcttc cctccacaga ttaatattcc tttacctcct aggccaaatt taaacagggg
     7921 ctttgatcaa caaggcctaa atccaacaac tttgaaggcc atcgggcaag caccttcaaa
     7981 tcttaccatg aatccttcca attttgctac cccacaaact cacaaattag attctgtggt
     8041 agtgaattct ggaaagcagt ctaattctgg agcaacaaaa cgggcaagtc caagcaacag
     8101 tcgcaggtct agtcctgggt ccagtaggaa aaccactcca agccctggga ggcaaaattc
     8161 aaaagcccct aaacttactc tggcctctca gacaaatgca gccctattgc agaatgtgga
     8221 gttgccgaga aatgtattgg tcagtcccac tcctctggcc aatccccctg tacctgggag
     8281 ctttcctaac aacagtgggc tgaatcctca gaattctact gtgtctgtgg ctgcagttgg
     8341 gggtgttgtt gaggataaca aggagagctt gaatgtgcct caggacagtg attgccagaa
     8401 ttcccagagt aggaaggaac aggtaaacat tgaactaaaa gcagtccctg cccaagaagt
     8461 taaaatggtt gtccctgaag atcagtccaa aaaggatggg cagccttcgg atcctaacaa
     8521 acttcccagt gtcgaagaga acaaaaattt ggtgtctcct gctatgaggg aagcaccaac
     8581 atcgttaagt caacttcttg acaactctgg agctcccaat gtgacaatta aaccccctgg
     8641 gcttacagat ctggaagtaa cacctccagt agtttctggg gaggacctca aaaaagcatc
     8701 tgtcattccc acactgcagg atctgtcttc ttctaaagaa ccttctaatt ccctaaactt
     8761 acctcacagt aatgagctgt gttcatccct tgtgcatccc gaattgagtg aggtcagttc
     8821 taacgttgca ccaagcatcc ctccagtaat gtcaagacct gttagctctt cctccatttc
     8881 cactcccttg cccccaaatc aaataactgt atttgtcact tccaatccca tcacaacttc
     8941 agctaacaca tcagcagctt tgccaactca cttgcagtct gcattgatgt caacagttgt
     9001 cacaatgccc aatgcgggta gcaaggttat ggtttctgag ggacagtcag ctgctcagtc
     9061 taatgcccgg cctcagttca ttacacctgt ctttatcaat tcatcctcaa taattcaggt
     9121 tatgaaagga tcacagccaa gcacaattcc tgcagcccca ctgacaacca actctggcct
     9181 gatgcctccc tctgttgcag ttgttggccc tttacacata cctcagaaca taaaattttc
     9241 ttctgctcct gtaccgccta atgccctctc cagtagtcct gctccaaaca tccagacagg
     9301 tcgacctttg gtccttagct cacgagccac ccctgttcag cttccttccc ctccttgtac
     9361 gtcttctcca gttgtccctt ctcatccccc tgtgcagcaa gtgaaagaat tgaatccaga
     9421 tgaggctagc cctcaggtga acacctcagc agatcagaac actcttccct cttcacagtc
     9481 aaccacaatg gtttctcccc ttttgaccaa tagtccaggg tcctctggca accggcgaag
     9541 cccagtctcg tctagtaagg gcaaaggaaa agtggacaaa attggccaaa ttttgttgac
     9601 caaggcatgt aagaaagtta caggctctct tgagaaaggg gaagaacaat atggtgcaga
     9661 tggagagact gaaggccaag ggctagacac cacagctccg gggctcatgg gaacagagca
     9721 gttatccaca gagctggaca gtaaaacccc aacgccccca gcacccactc tgctaaaaat
     9781 gacctctagc cctgtgggcc cgggcactgc ctcagcagga cccagcttac ctggcggtgc
     9841 tctccccacc agtgtacgct cgatagtaac cactctggta ccctccgagc tcatctccgc
     9901 cgtaccgacc acaaaaagca atcatggtgg catagcatct gagtcacttg cgggtggcct
     9961 agtggaggag aaggtgggat cccatccaga acttctaccc agcatagccc cgtcgcagaa
    10021 tttagtctca aaggaaactt caaccacagc actgcaggcc tctgttgcca gaccagagct
    10081 ggaggtaaat gctgccatag tctctggaca aagcagtgag cccaaagaga tagttgaaaa
    10141 gtccaaaatc ccaggccgaa gaaactcccg aactgaagag ccaactgtgg cctctgaaag
    10201 tgtggaaaat ggacatcgta aacgatcttc tcgacctgct tcagcctcca gctctactaa
    10261 agacataacc agtgcggtgc aatccaagcg aagaaaatcc aagtagtaat gaactagtcc
    10321 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgcagt
    10381 agagttaaag cctacagcac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
    10441 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    10501 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    10561 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    10621 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    10681 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    10741 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    10801 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    10861 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10921 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10981 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
    11041 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    11101 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    11161 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    11221 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    11281 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
    11341 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
    11401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    11461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
    11521 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    11581 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    11641 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
    11701 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    11761 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    11821 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    11881 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    11941 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    12001 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    12061 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    12121 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    12181 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    12241 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    12301 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    12361 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    12421 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    12481 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    12541 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    12601 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    12661 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    12721 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    12781 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    12841 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    12901 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    12961 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    13021 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    13081 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    13141 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    13201 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    13261 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    13321 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    13381 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    13441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    13501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    13561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    13621 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    13681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    13741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    13801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    13861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    13921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    13981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    14041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    14101 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    14161 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    14221 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    14281 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    14341 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    14401 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    14461 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    14521 accctcacta aagggaacaa aagctggagc tgcaagc
//
