LOCUS       sequence-404305-       10206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3250
FEATURES             Location/Qualifiers
     source          1..10206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5880..5904)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5912..5953
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6063..6651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6116..6136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6534..6545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6723..6956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7034..7155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7071..7090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7125..7144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7195..7330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7350..7369)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7351..7369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7379..7396)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7379..7395)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7388..7410)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7537..7992
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7624..7643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7834..7855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8018..8122
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8123..8191,8192..8983)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8341..8360)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9154..9742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9643..9662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9896..9913
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10030..10051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10066..10071,10072..10089,10090..10096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10104..10120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10109..10131
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10128..10144
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10128..10144
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10163..10183
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10165..10183
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     4141 caccatggct gaagaatcaa gaaagccttc agccccatcc ccaccagacc agactcctga
     4201 agaggatctt gtaatcgtca aggtagagga ggatcatggt tgggaccagg aatctagtct
     4261 gcatgaaagt aaccctcttg gccaagaagt gttccgcctg cgcttcaggc agttacgcta
     4321 ccaggagaca ctaggacccc gagaagctct gatccaacta cgggcccttt gccatcagtg
     4381 gctgaggcca gatttgaaca ccaaggaaca gatcctggag ctgctggtgc tggagcagtt
     4441 cttgaccatc ctacctgagg agctccagac actggttaag gaacatcagc tagagaacgg
     4501 agaggaggtg gtgaccctat tagaggattt ggaaaggcag attgatatac taggacgacc
     4561 agtctcagct cgcgtacatg gacatagggt actctgggag gaggtagtac attcagcatc
     4621 tgcaccagag cctccaaata ctcagctcca atctgaggca acccaacata aatctccagt
     4681 gccccaagag tcacaagaga gagccatgtc tacttcccag agtcctactc gttcccagaa
     4741 aggaagttct ggagaccagg aaatgacagc tacacttctc acagcagggt tccagacttt
     4801 ggagaagatt gaagacatgg ctgtgtccct tattcgagag gagtggcttc ttgatccatc
     4861 acagaaggat ctgtgtagag ataacaggcc agaaaatttc agaaacatgt tctccctggg
     4921 tggtgagacc aggagtgaga acagggaatt agcttcaaaa caggtaatat ctactggaat
     4981 ccagccacat ggagagacag ctgccaaatg caacggggat gttatcaggg gtcttgagca
     5041 tgaagaagcc cgagaccttc tgggcagatt agagaggcag cggggaaatc ccacacaaga
     5101 gagacgacat aaatgtgatg aatgtgggaa aagctttgct cagagctcag gccttgttcg
     5161 ccactggaga atccacactg gggagaaacc ctatcagtgt aatgtgtgtg gtaaagcctt
     5221 cagttacagg tcagcccttc tttcacatca ggatatccac aacaaagtaa aacgctatca
     5281 ctgtaaggag tgtggcaaag ccttcagtca gaacacaggc ctgattctgc accagagaat
     5341 ccacactggg gagaagccat atcagtgcaa tcagtgtggg aaggctttca gtcagagtgc
     5401 gggccttatt ctgcaccaga gaatccacag tggagagaga ccctatgaat gtaatgagtg
     5461 tgggaaagct ttcagtcata gctcacacct cattggacat cagagaatcc acactgggga
     5521 gaagccctat gagtgtgatg agtgtgggaa aaccttcagg cggagctcac atcttattgg
     5581 tcatcagagg agccacactg gggagaaacc ctacaaatgc aatgagtgtg ggagggcctt
     5641 cagtcagaag tcaggcctta ttgaacatca gagaatccac actggagaaa gaccctataa
     5701 atgtaaagaa tgtgggaaag ctttcaatgg gaacactggt ctcattcaac acctgagaat
     5761 tcacacaggg gagaagccct accaatgtaa tgagtgtggg aaagccttta ttcagaggtc
     5821 aagtctcatt cgacatcaga gaatccacag tggtgaaaaa tctgaatcca taagcgtttt
     5881 gccaactttc ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg
     5941 tctcgattct acgtagtaat gaactagtcc gtaacttgaa agtatttcga ttcttggctt
     6001 tatatatctt gtggaaagga cgacgggctc gaaacttatc cgtggacacg cgttaagtcg
     6061 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6121 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6181 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6241 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6301 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6361 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6421 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6481 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6541 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6601 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     6661 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6721 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6781 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6841 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6901 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     6961 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7021 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7081 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7141 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7201 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7261 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7321 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     7381 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7441 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7501 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     7561 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7621 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7681 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7741 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7801 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7861 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7921 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7981 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8041 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8101 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8161 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8221 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8281 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8341 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8401 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8461 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8521 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8581 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8641 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8701 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8761 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8821 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8881 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8941 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9001 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9061 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9121 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9181 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9241 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9301 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9361 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9421 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9481 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9541 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9601 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9661 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9721 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9781 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9841 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9901 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9961 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10021 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10081 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10141 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
    10201 gcaagc
//
