LOCUS       sequence-404313-       10090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3242
FEATURES             Location/Qualifiers
     source          1..10090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5763..5787)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5795..5836
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5947..6535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6000..6020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6418..6429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6607..6840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6918..7039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6955..6974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7009..7028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7079..7214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7234..7253)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7235..7253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7263..7280)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7263..7279)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7272..7294)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7421..7876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7508..7527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7718..7739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7902..8006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8007..8075,8076..8867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8225..8244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9038..9626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9527..9546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9780..9797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9914..9935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9950..9955,9956..9973,9974..9980)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9988..10004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9993..10015
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10012..10028
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10012..10028
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10047..10067
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10049..10067
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     4141 catggactcg gtggcttttg aggatgtaga tgtgaacttc acccaggagg agtgggcttt
     4201 gctagatcct tcccagaaga atctctacag agatgtgatg tgggaaacca tgaggaatct
     4261 ggcctctata gggaaaaaat ggaaggacca gaacattaaa gatcactaca aacaccgagg
     4321 gagaaatcta agaagtcata tgttagaaag actctatcaa actaaggatg gtagtcagcg
     4381 tggaggaatt tttagccagt ttgcaaatca gaatctgagc aagaaaatcc ctggagtgaa
     4441 actctgtgaa agcattgtat atggagaagt cagcatgggt cagtcatccc ttaatagaca
     4501 catcaaagat cacagtggac atgaaccaaa ggaatatcag gaatatggag agaagccaga
     4561 tacacgtaac cagtgttgga aacccttcag ttctcaccac tcctttcgaa cacatgagat
     4621 aattcacact ggagagaaac tctatgattg taaggaatgt ggaaaaacct tcttttctct
     4681 caaaagaatt agaagacaca tcatcacaca cagtggatat acaccatata aatgtaaggt
     4741 gtgtgggaaa gcttttgatt atcccagtag atttcgaaca catgaaagaa gtcacactgg
     4801 agagaaaccc tatgaatgtc aggaatgtgg aaaagccttc acttgtatca caagtgttcg
     4861 aagacacatg ataaagcaca ctggagatgg accttataaa tgtaaggtat gtgggaaacc
     4921 ctttcattct ctgagttcat ttcaagtgca tgaaagaatt cacactggag aaaaaccctt
     4981 taaatgtaag caatgtggta aagccttcag ttgttcccca accttacgaa tacatgaaag
     5041 aacccatact ggagagaaac cttatgaatg caagcagtgt gggaaggcct tcagttatct
     5101 cccctccctt cgactacatg aaagaattca cactggtgag aaacccttcg tatgtaaaca
     5161 atgtggtaaa gcctttagat ctgccagtac ctttcaaata catgaaagga ctcacactgg
     5221 agaaaaacct tatgaatgta aggaatgtgg ggaagcattc agttgtatcc caagtatgcg
     5281 aagacacatg ataaaacata ctggagaagg accttataaa tgtaaggtat gtgggaaacc
     5341 ctttcattct ctgagtccat ttcgaataca tgaaagaact cacactggag agaaacctta
     5401 tgtatgtaaa cattgtggta aagctttcgt ttcttcaaca tcaattcgaa tacatgaaag
     5461 aactcatact ggagagaaac cctatgagtg taagcaatgt gggaaagcct tcagttatct
     5521 caactccttt cgaacacatg aaatgattca cactggtgag aaaccctttg aatgtaagcg
     5581 atgtggtaaa gcctttagat cttctagttc ctttcgacta catgaaagga ctcacactgg
     5641 acagaaaccc tatcattgca aggaatgtgg gaaagcctat tcttgccgtg ccagctttca
     5701 gagacacatg ttaacacatg ctgaagatgg accaccttat aaatgcatgt gggaaagcct
     5761 ttacccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     5821 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     5881 gctttatata tcttgtggaa aggacgactc actgagcgga cgcgattacc tacgcgttaa
     5941 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6001 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6061 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6121 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6181 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6241 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6301 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6361 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6421 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6481 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     6541 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6601 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6841 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6901 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6961 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7021 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7081 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7141 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7201 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7261 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7321 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7381 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7441 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7501 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7561 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7621 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7681 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7861 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8161 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8221 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8521 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8581 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8641 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8701 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8761 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8821 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8881 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8941 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9901 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9961 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10021 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    10081 agctgcaagc
//
