LOCUS       sequence-404506-       12130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3462
FEATURES             Location/Qualifiers
     source          1..12130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7803..7827)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7835..7876
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7987..8575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8040..8060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8458..8469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8647..8880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8958..9079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8995..9014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9049..9068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9119..9254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9274..9293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9275..9293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9303..9320)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9303..9319)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9312..9334)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9461..9916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9548..9567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9758..9779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9942..10046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10047..10115,10116..10907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10265..10284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11078..11666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11567..11586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11820..11837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11954..11975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11990..11995,11996..12013,12014..12020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12028..12044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12033..12055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12052..12068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12052..12068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12087..12107
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12089..12107
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     4141 catgggggtg gactttgatg tgaagacttt ctgccacaac ttgcgggcga ctaagccacc
     4201 atacgagtgc ccggtggaga cctgccgaaa ggtctacaag agttacagtg gtattgagta
     4261 ccacctgtac cactatgacc acgacaaccc accaccccca caacaaactc cactccgcaa
     4321 gcacaagaaa aaggggcgcc agtcacgccc agccaacaag cagtcaccca gcccctcaga
     4381 ggtctcacag tcaccaggcc gtgaggtgat gagctatgca caggcccagc gcatggtgga
     4441 ggtggacttg catggccgcg tccaccgcat cagcatcttt gacaacctgg atgtggtgtc
     4501 agaggatgag gaagcccccg aggaggcccc tgagaatggc agcaacaagg agaacactga
     4561 gacaccagct gctactccca agtcaggcaa acataagaac aaggagaagc gcaaggactc
     4621 caaccatcat caccaccaca atgtttctgc gagcaccact cccaagctgc cagaggtggt
     4681 ctatcgggag ctggaacagg acacccctga tgccccaccc cggccaactt cctattaccg
     4741 gtacatcgag aagtctgcag aggagctgga cgaggaagta gagtatgaca tggacgagga
     4801 ggactacatc tggctggata tcatgaatga gcgtcggaag acagagggtg taagtcccat
     4861 cccgcaggag atctttgagt acctaatgga ccgactggag aaggagtcgt actttgagag
     4921 tcataataaa ggcgacccta atgcgctagt ggacgaggat gctgtttgct gtatctgcaa
     4981 tgatggtgag tgccagaaca gcaatgtcat cctcttctgt gacatgtgca acctggccgt
     5041 gcaccaggag tgctacggtg tcccctatat ccctgagggc cagtggctgt gccgccgttg
     5101 cctgcagtca ccctctcgtg ctgtggattg tgccctgtgc cccaacaagg gcggtgcctt
     5161 caagcagaca gatgacgggc gctgggccca tgtggtgtgt gccttgtgga tccctgaggt
     5221 ctgcttcgcc aacacggtct tcctagagcc tattgacagc attgagcaca tcccaccagc
     5281 tcgctggaag ctcacctgct acatttgcaa acaacggggc tcaggggcct gcatccagtg
     5341 ccacaaggcc aactgttaca cagctttcca tgtgacatgc gcccagcagg ctggccttta
     5401 catgaagatg gagcctgtgc gggagacagg cgccaacggc acctctttca gtgtccgcaa
     5461 gacagcctac tgcgacatcc acacgcctcc aggttcagca cgccgactgc ctgccctgtc
     5521 ccacagcgag ggtgaggagg atgaagatga ggaggaggat gagggtaagg gctggagctc
     5581 agagaaagtc aagaaggcca aggccaagtc ccggatcaaa atgaagaagg cacggaagat
     5641 cctggcagag aagcgggcag cagcacctgt ggtgtcagtg ccctgcatcc caccacacag
     5701 gcttagtaaa atcaccaacc gcctgaccat ccaaaggaag agccagttca tgcagaggct
     5761 gcacagctac tggacactga agcggcagtc acggaatggg gtcccattgc tacgtcgcct
     5821 gcagacacac ctgcaatctc agaggaactg tgaccaagtt gggagagatt ctgaggataa
     5881 gaactgggcc cttaaagaac agctcaagtc atggcagcgg ctccggcatg acttggagcg
     5941 agctcggctg ctcgtggaat tgatccgcaa gcgggaaaaa ctcaaaaggg agacgatcaa
     6001 ggttcagcag attgccatgg agatgcagct gactcctttc ctcatcctcc ttcgcaaaac
     6061 cttggagcag ctccaagaga aggacacagg caacatcttc agcgagccgg tccctctgtc
     6121 tgaggtaacc gaattggacg aagtacctga ctacctagac cacatcaaaa agcccatgga
     6181 ctttttcacc atgaagcaga acttggaggc ttaccgctac ctgaattttg atgattttga
     6241 ggaggacttc aacctcatcg tcagcaactg cctcaagtat aacgccaagg acaccatctt
     6301 ctaccgggca gcagtgcggc ttcgtgagca gggtggtgct gtgctccgcc aggcccggcg
     6361 ccaggcagaa aaaatgggca ttgactttga gacgggcatg catatccccc acagcctggc
     6421 tggagatgag gccacacacc acactgaaga tgcagccgag gaagagcggc tggtcttgct
     6481 ggagaaccag aagcacctgc cagtggaaga acagctaaag ctgcttctgg agcggctgga
     6541 cgaagtgaat gccagcaagc agagtgtggg ccgctcacgg cgtgcaaaga tgatcaagaa
     6601 agagatgacg gcactgcggc ggaagcttgc ccatcagcga gagacgggac gtgatggccc
     6661 tgagcggcat ggcccctcga gccggggtag tctgacaccc cacccggcag cctgtgacaa
     6721 ggatgggcag acagatagtg cggcagagga gagcagcagc caggagacaa gcaaaggcct
     6781 gggtcccaac atgtcctcaa cccccgcaca tgaggtgggc aggagaacct cagttctgtt
     6841 ctccaaaaag aacccgaaga cagctggacc gcccaagagg ccgggccggc cccccaaaaa
     6901 ccgggagagc cagatgaccc ccagccacgg aggcagtcct gtggggcccc cccagctccc
     6961 catcatgagt tccctgcgtc agcgcaagcg gggtaggagc ccccggccca gttcgagctc
     7021 agacagcgac agtgataagt ccacagaaga ccccccaatg gacttaccag ccaatggctt
     7081 cagcggtgga aaccaaccag tgaagaagag tttcttggta taccgtaatg actgcagcct
     7141 tccccggagc agctcagact ctgagtccag cagcagtagc agtagcagcg ctgcttcaga
     7201 ccggaccagc acaacgccct caaaacaagg ccggggcaaa ccctccttct ctcggggcac
     7261 tttcccagag gacagcagtg aggatacctc aggcactgag aatgaggcct actccgtggg
     7321 cactggccgc ggcgtgggcc acagcatggt aaggaagagt ctgggccggg gagctggctg
     7381 gctgtcagag gatgaggact ccccgctgga tgctctggac ctcgtgtggg ccaaatgccg
     7441 aggctatcca tcatacccag ctctgatcat tgatccaaag atgccccgag aaggtatgtt
     7501 ccaccatggg gttcccatcc ctgtgccccc actggaggtg ctgaaacttg gggagcagat
     7561 gacccaggaa gcccgagagc atctctacct cgtcctcttc tttgacaaca aacgaacctg
     7621 gcagtggctg cccaggacca agctggttcc tctgggtgtg aaccaggacc tagacaagga
     7681 gaagatgctg gagggccgca agtccaacat ccgcaagtca gtacagatcg cctaccacag
     7741 ggctctgcag caccgcagca aggtgcaagg cgagcagagc agtgagacca gtgatagtga
     7801 ttgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     7861 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     7921 gctttatata tcttgtggaa aggacgacta tgcaaatagt tgcacttatt aacgcgttaa
     7981 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8521 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     8581 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     8641 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     8701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8881 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8941 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9061 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     9121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9181 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9241 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     9301 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9361 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9421 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     9481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     9901 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    11941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12061 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12121 agctgcaagc
//
