LOCUS       sequence-404538-       10681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3497
FEATURES             Location/Qualifiers
     source          1..10681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4256..4267
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(6354..6378)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6386..6427
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6538..7126
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6591..6611)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7009..7020)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7198..7431
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7509..7630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7546..7565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7600..7619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7670..7805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7825..7844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7826..7844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7854..7871)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7854..7870)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7863..7885)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8012..8467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8099..8118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8309..8330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8493..8597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8598..8666,8667..9458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8816..8835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9629..10217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10118..10137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10371..10388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10505..10526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10541..10546,10547..10564,10565..10571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10579..10595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10584..10606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10603..10619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10603..10619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10638..10658
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10640..10658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     4141 catggatgac gacaaacctt ttcaaccaaa aaacatttca aaaatggcag aactctttat
     4201 ggaatgtgaa gaagaagagc tagagccatg gcagaagaaa gtagaagaaa ctcaggatga
     4261 ggatgacgat gaactgatct ttgttggaga gatatcaagt tcaaaaccag ccatttcaaa
     4321 tattttgaac agaggccact ccagctcatc ttcaaaagga ataaagagtg agccacacag
     4381 tccaggtatt cctgaaatat tcaggactgc aagtcaacgc tgcagagacc caccatcaaa
     4441 tccagtggct gcctcgccta gatttcatct tgtatctaaa tcttcacaaa gctctgttac
     4501 tgttgagaat gcgtctaaac ctgattttac aaagaattca caagttggat cggataattc
     4561 ttcaatttta ctgtttgact cgacccagga atcactacca ccatcccaag acataccagc
     4621 aatttttaga gaaggtatga aaaatacttc atatgtgttg aaacatcctt ctacttctaa
     4681 agtaaacagt gttactccaa aaaaaccaaa gaccagtgaa gatgttcctc agataaatcc
     4741 ctccacttca ttgcctttaa ttggctctcc tccagtgaca tcctcccaag ttatgctgtc
     4801 aaaaggtaca aatacctcat ctccatatga tgctggagca gattacctaa gagcttgtcc
     4861 aaagtgcaat gttcagttca atcttttgga tcctttgaaa taccacatga agcattgttg
     4921 tccagacatg ataactaaat ttttgggagt aattgttaaa tcagaacgtc catgtgatga
     4981 agacaagact gattcagaga caggaaagtt gatcatgtta gtcaatgagt tttattatgg
     5041 aaggcatgaa ggagtcactg agaaagagcc aaagacttac acaaccttta aatgcttcag
     5101 ttgctcgaaa gttcttaaaa ataatattag gtttatgaac cacatgaaac atcacttgga
     5161 acttgagaag cagaacaatg aaagctggga aaaccacacc acctgccagc actgttaccg
     5221 gcaatatccc acacccttcc aactgcagtg ccacattgag agtacacaca ctccccatga
     5281 gttttctact atttgcaaaa tctgtgaatt atcatttgaa acagagcata ttcttttaca
     5341 acatatgaag gacacccata aacctggtga aatgccatat gtttgccagg tttgccagtt
     5401 tagatcatca acattttctg atgtagaagc tcattttaga gcagcccatg aaaacactaa
     5461 gaacttgcta tgtccatttt gcctcaaagt tagtaaaatg gcaaccccct acatgaatca
     5521 ttacatgaag catcagaaaa aaggagttca tcgttgccca aaatgcagac tacaattttt
     5581 gaccagcaag gagaaagctg aacataaggc gcagcatcgt acatttataa agcctaaaga
     5641 actagaagga ttgcctcctg gagcaaaagt tactattcga gcttcacttg gacctctcca
     5701 gtcaaaatta ccaactgcac ctttcggttg cgctccaggc acttcttttc ttcaggtcac
     5761 acctccgaca tctcaaaata caactgctag aaatcctaga aaatctaatg ccagtagatc
     5821 taagacaagt aagcttcatg caactacatc cactgcaagt aaagttaata caagtaagcc
     5881 aaggggacgt atagctaagt ccaaagcaaa accctcttac aagcaaaagc gacagcgcaa
     5941 cagaaaaaat aaaatgagcc ttgctttgaa gaacataagg tgtcgtcggg gcattcacaa
     6001 gtgcattgaa tgtcattcca aaataaaaga ttttgcaagc cacttttcta tatacatcca
     6061 ctgcagtttt tgcaagtaca atactaactg taacaaagcc tttgtaaatc atatgatgag
     6121 ctctcatagc aaccatccag gtaaacggtt ttgtattttc aagaagcatt caggaactct
     6181 caggggcatt actctagtgt gccttaaatg tgatttccta gctgattctt ccggcttaga
     6241 tcgtatggct aaacacttaa gtcaacgtaa aactcatact tgccaagtta taatagagaa
     6301 tgtttccaaa agtacctcaa cttctgaacc cactactggc tgctcattga aatacccaac
     6361 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     6421 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     6481 atcttgtgga aaggacgata gaaagaccct tccttctacc caacgcgtta agtcgacaat
     6541 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6601 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6661 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6721 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6781 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6841 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6901 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6961 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7021 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7081 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     7141 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7201 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7261 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7321 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7381 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     7441 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7501 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7561 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7621 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     7681 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7741 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7801 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7861 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7921 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7981 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8041 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8101 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8161 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8221 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8281 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8341 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8401 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8461 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8521 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8581 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8641 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8701 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8761 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8821 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8881 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8941 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9001 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9061 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9121 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9181 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9241 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9301 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9361 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9421 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10321 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10381 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10441 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10501 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10561 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10621 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    10681 c
//
