LOCUS       sequence-404553-       13216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0390
FEATURES             Location/Qualifiers
     source          1..13216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9073..9661
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9126..9146)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9544..9555)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9733..9966
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10044..10165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10081..10100)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10135..10154
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10205..10340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10360..10379)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10361..10379)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10389..10406)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10389..10405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10398..10420)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10547..11002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10634..10653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10844..10865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11028..11132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11133..11201,11202..11993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11351..11370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12164..12752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12653..12672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12906..12923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13040..13061
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13076..13081,13082..13099,13100..13106)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13114..13130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13119..13141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13138..13154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13138..13154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13173..13193
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13175..13193
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtcc actccagacc cacccctggg
     4141 cggaactcct cggccaggtc cttccccggg ccctggccct tcccctggag ccatgctggg
     4201 ccctagcccg ggtccctcgc cgggctccgc ccacagcatg atggggccca gcccagggcc
     4261 gccctcagca ggacacccca tccccaccca ggggcctgga gggtaccctc aggacaacat
     4321 gcaccagatg cacaagccca tggagtccat gcatgagaag ggcatgtcgg acgacccgcg
     4381 ctacaaccag atgaaaggaa tggggatgcg gtcagggggc catgctggga tggggccccc
     4441 gcccagcccc atggaccagc actcccaagg ttacccctcg cccctgggtg gctctgagca
     4501 tgcctctagt ccagttccag ccagtggccc gtcttcgggg ccccagatgt cttccgggcc
     4561 aggaggtgcc ccgctggatg gtgctgaccc ccaggccttg gggcagcaga accggggccc
     4621 aaccccattt aaccagaacc agctgcacca gctcagagct cagatcatgg cctacaagat
     4681 gctggccagg gggcagcccc tccccgacca cctgcagatg gcggtgcagg gcaagcggcc
     4741 gatgcccggg atgcagcagc agatgccaac gctacctcca ccctcggtgt ccgcaacagg
     4801 acccggccct ggccctggcc ctggccccgg cccgggtccc ggcccggcac ctccaaatta
     4861 cagcaggcct catggtatgg gagggcccaa catgcctccc ccaggaccct cgggcgtgcc
     4921 ccccgggatg ccaggccagc ctcctggagg gcctcccaag ccctggcctg aaggacccat
     4981 ggcgaatgct gctgccccca cgagcacccc tcagaagctg attcccccgc agccaacggg
     5041 ccgcccttcc cccgcgcccc ctgccgtccc acccgccgcc tcgcccgtga tgccaccgca
     5101 gacccagtcc cccgggcagc cggcccagcc cgcgcccatg gtgccactgc accagaagca
     5161 gagccgcatc acccccatcc agaagccgcg gggcctcgac cctgtggaga tcctgcagga
     5221 gcgcgagtac aggctgcagg ctcgcatcgc acaccgaatt caggaacttg aaaaccttcc
     5281 cgggtccctg gccggggatt tgcgaaccaa agcgaccatt gagctcaagg ccctcaggct
     5341 gctgaacttc cagaggcagc tgcgccagga ggtggtggtg tgcatgcgga gggacacagc
     5401 gctggagaca gccctcaatg ctaaggccta caagcgcagc aagcgccagt ccctgcgcga
     5461 ggcccgcatc actgagaagc tggagaagca gcagaagatc gagcaggagc gcaagcgccg
     5521 gcagaagcac caggaatacc tcaatagcat tctccagcat gccaaggatt tcaaggaata
     5581 tcacagatcc gtcacaggca aaatccagaa gctgaccaag gcagtggcca cgtaccatgc
     5641 caacacggag cgggagcaga agaaagagaa cgagcggatc gagaaggagc gcatgcggag
     5701 gctcatggct gaagatgagg aggggtaccg caagctcatc gaccagaaga aggacaagcg
     5761 cctggcctac ctcttgcagc agacagacga gtacgtggct aacctcacgg agctggtgcg
     5821 gcagcacaag gctgcccagg tcgccaagga gaaaaagaag aaaaagaaaa agaagaaggc
     5881 agaaaatgca gaaggacaga cgcctgccat tgggccggat ggcgagcctc tggacgagac
     5941 cagccagatg agcgacctcc cggtgaaggt gatccacgtg gagagtggga agatcctcac
     6001 aggcacagat gcccccaaag ccgggcagct ggaggcctgg ctcgagatga acccggggta
     6061 tgaagtagct ccgaggtctg atagtgaaga aagtggctca gaagaagagg aagaggagga
     6121 ggaggaagag cagccgcagg cagcacagcc tcccaccctg cccgtggagg agaagaagaa
     6181 gattccagat ccagacagcg atgacgtctc tgaggtggac gcgcggcaca tcattgagaa
     6241 tgccaagcaa gatgtcgatg atgaatatgg cgtgtcccag gcccttgcac gtggcctgca
     6301 gtcctactat gccgtggccc atgctgtcac tgagagagtg gacaagcagt cagcgcttat
     6361 ggtcaatggt gtcctcaaac agtaccagat caaaggtttg gagtggctgg tgtccctgta
     6421 caacaacaac ctgaacggca tcctggccga cgagatgggc ctggggaaga ccatccagac
     6481 catcgcgctc atcacgtacc tcatggagca caaacgcatc aatgggccct tcctcatcat
     6541 cgtgcctctc tcaacgctgt ccaactgggc gtacgagttt gacaagtggg ccccctccgt
     6601 ggtgaaggtg tcttacaagg gatccccagc agcaagacgg gcctttgtcc cccagctccg
     6661 gagtgggaag ttcaacgtct tgctgacgac gtacgagtac atcatcaaag acaagcacat
     6721 cctcgccaag atccgttgga agtacatgat tgtggacgaa ggtcaccgca tgaagaacca
     6781 ccactgcaag ctgacgcagg tgctcaacac gcactatgtg gcaccccgcc gcctgctgct
     6841 gacgggcaca ccgctgcaga acaagcttcc cgagctctgg gcgctgctca acttcctgct
     6901 gcccaccatc ttcaagagct gcagcacctt cgagcagtgg tttaacgcac cctttgccat
     6961 gaccggggaa aaggtggacc tgaatgagga ggaaaccatt ctcatcatcc ggcgtctcca
     7021 caaagtgctg cggcccttct tgctccgacg actcaagaag gaagtcgagg cccagttgcc
     7081 cgaaaaggtg gagtacgtca tcaagtgcga catgtctgcg ctgcagcgag tgctctaccg
     7141 ccacatgcag gccaagggcg tgctgctgac tgatggctcc gagaaggaca agaagggcaa
     7201 aggcggcacc aagaccctga tgaacaccat catgcagctg cggaagatct gcaaccaccc
     7261 ctacatgttc cagcacatcg aggagtcctt ttccgagcac ttggggttca ctggcggcat
     7321 tgtccaaggg ctggacctgt accgagcctc gggtaaattt gagcttcttg atagaattct
     7381 tcccaaactc cgagcaacca accacaaagt gctgctgttc tgccaaatga cctccctcat
     7441 gaccatcatg gaagattact ttgcgtatcg cggctttaaa tacctcaggc ttgatggaac
     7501 cacgaaggcg gaggaccggg gcatgctgct gaaaaccttc aacgagcccg gctctgagta
     7561 cttcatcttc ctgctcagca cccgggctgg ggggctcggc ctgaacctcc agtcggcaga
     7621 cactgtgatc atttttgaca gcgactggaa tcctcaccag gacctgcaag cgcaggaccg
     7681 agcccaccgc atcgggcagc agaacgaggt gcgtgtgctc cgcctctgca ccgtcaacag
     7741 cgtggaggag aagatcctag ctgcagccaa gtacaagctc aacgtggacc agaaggtgat
     7801 ccaggccggc atgttcgacc agaagtcctc cagccatgag cggcgcgcct tcctgcaggc
     7861 catcctggag cacgaggagc aggatgagga ggaagacgag gtgcccgacg acgagaccgt
     7921 caaccagatg atcgcccggc acgaggagga gtttgatctg ttcatgcgca tggacctgga
     7981 ccgcaggcgc gaggaggccc gcaaccccaa gcggaagccg cgcctcatgg aggaggacga
     8041 gctcccctcg tggatcatca aggacgacgc ggaggtggag cggctgacct gtgaggagga
     8101 ggaggagaag atgttcggcc gtggctcccg ccaccgcaag gaggtggact acagcgactc
     8161 actgacggag aagcagtggc tcaagaccct gaaggccatc gaggagggca cgctggagga
     8221 gatcgaagag gaggtccggc agaagaaatc atcacggaag cgcaagcgag acagcgacgc
     8281 cggctcctcc accccgacca ccagcacccg cagccgcgac aaggacgacg agagcaagaa
     8341 gcagaagaag cgcgggcggc cgcctgccga gaaactctcc cctaacccac ccaacctcac
     8401 caagaagatg aagaagattg tggatgccgt gatcaagtac aaggacagca gtggacgtca
     8461 gctcagcgag gtcttcatcc agctgccctc gcgaaaggag ctgcccgagt actacgagct
     8521 catccgcaag cccgtggact tcaagaagat aaaggagcgc attcgcaacc acaagtaccg
     8581 cagcctcaac gacctagaga aggacgtcat gctcctgtgc cagaacgcac agaccttcaa
     8641 cctggagggc tccctgatct atgaagactc catcgtcttg cagtcggtct tcaccagcgt
     8701 gcggcagaaa atcgagaagg aggatgacag tgaaggcgag gagagtgagg aggaggaaga
     8761 gggcgaggag gaaggctccg aatccgaatc tcggtccgtc aaagtgaaga tcaagcttgg
     8821 ccggaaggag aaggcacagg accggctgaa gggcggccgg cggcggccga gccgagggtc
     8881 ccgagccaag ccggtcgtga gtgacgatga cagtgaggag gaacaagagg aggaccgctc
     8941 aggaagtggc agcgaagaag actagtaatg aactagtccg taacttgaaa gtatttcgat
     9001 ttcttggctt tatatatctt gtggaaagga cgacatctcc cactatctgg ttgaggaacg
     9061 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9121 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9181 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9241 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9301 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9361 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9421 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9481 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9541 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9601 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9661 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9721 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9781 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9841 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9901 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9961 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    10021 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    10081 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10141 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    10201 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10261 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10321 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    10381 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    10441 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    10501 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    10561 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    10621 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10681 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10741 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10801 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10861 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10921 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10981 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    11041 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11101 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11161 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11221 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11281 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11341 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11401 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11461 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11521 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11581 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11641 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11701 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11761 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11821 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11881 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11941 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    12001 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    12061 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    12241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12301 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12841 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12901 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12961 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    13021 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    13081 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    13141 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    13201 agctggagct gcaagc
//
