LOCUS       sequence-404562-       11122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0909
FEATURES             Location/Qualifiers
     source          1..11122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6979..7567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7032..7052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7450..7461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7639..7872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7950..8071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7987..8006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8041..8060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8111..8246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8266..8285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8267..8285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8295..8312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8295..8311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8304..8326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8453..8908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8540..8559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8750..8771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8934..9038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9039..9107,9108..9899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9257..9276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10070..10658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10559..10578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10812..10829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10946..10967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10982..10987,10988..11005,11006..11012)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11020..11036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11025..11047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11044..11060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11044..11060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11079..11099
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11081..11099
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggt cgctccaatt ctagatcaca
     4141 ttcttcaagg tcaaagtcta gatcacagtc tagttctcga tcaagatcaa gatctcattc
     4201 tagaaagaag cgatacaggt ctcgttccag aacatattca aggtctcgta gtagagatcg
     4261 tatgtattct agagattatc gtcgcgatta cagaaataat agaggaatga gacgacctta
     4321 tgggtacaga ggaaggggta gagggtatta tcaaggagga ggaggtagat atcatcgagg
     4381 tggttataga cctgtctgga atagaaggca ctctaggagt cctagacgag gtcgttcacg
     4441 ttccaggagt ccaaaaagaa gatccgtttc ttctcaaaga tccagaagca gatctcgccg
     4501 gtcatataga tcttctaggt ctccaagatc atcctcttct cgttcttcat ccccatatag
     4561 caaatctcct gtttctaaaa gacgagggtc tcaggaaaaa caaaccaaaa aagctgaagg
     4621 ggaaccccaa gaagagagtc cgttgaaaag taaatcacag gaggaaccga aagatacatt
     4681 tgaacatgac ccatctgagt ctatcgatga atttaataag tcatcagcca catccggtga
     4741 tatttggcct ggcctttcag cttatgataa tagtcctaga tcaccccata gtccttcacc
     4801 tattgctaca ccacctagtc agagttcatc ttgctctgat gctcccatgc tcagtacagt
     4861 tcactctgca aaaaatactc cttctcagca ttcacattcc attcagcata gtcctgaaag
     4921 gtctgggtct ggttctgttg gaaatggatc tagtcgatac agtccttctc agaatagtcc
     4981 aattcatcac atcccttcac gaagaagtcc tgcaaagaca atcgcaccac agaatgctcc
     5041 aagagatgag tctaggggcc gttcctcgtt ttatcctgat ggtggagatc aggaaactgc
     5101 aaagactggg aagttcttaa aaaggttcac agatgaagag tctagagtat tcctgcttga
     5161 taggggtaat accagggata aagaggcttc aaaagagaaa ggatcagaga aagggagggc
     5221 agagggagaa tgggaagatc aggaagctct agattacttc agtgataaag agtctggaaa
     5281 acaaaagttt aatgattcag aaggggatga cacagaggag acagaggatt atagacagtt
     5341 caggaagtca gtcctcgcag atcagggtaa aagttttgct actgcatctc accggaatac
     5401 tgaggaggaa ggactcaagt acaagtccaa agtttcactg aaaggcaata gagaaagtga
     5461 tggatttaga gaagaaaaaa attataaact taaagagact ggatatgtag tggaaaggcc
     5521 tagcactaca aaagataagc acaaagaaga agacaaaaat tctgaaagaa taacagtaaa
     5581 gaaagaaact cagtcacctg agcaggtaaa gtctgaaaag ctcaaagacc tctttgatta
     5641 cagtccccct ctacacaaga atctggatgc acgagaaaag tctaccttca gagaggaaag
     5701 cccacttagg atcaaaatga tagcgagtga ttctcaccgt cctgaagtca aactcaaaat
     5761 ggcacctgtt cctcttgatg attctaacag acctgcttcc ttgactaaag acaggctgct
     5821 tgctagtaca cttgtccatt ctgtcaagaa ggagcaagaa ttccgatcca tctttgacca
     5881 cattaagttg ccacaggcca gcaaaagcac ttcagagtca tttattcaac acattgtgtc
     5941 cttggttcat catgttaaag agcaatactt caagtcagct gcaatgaccc taaacgagcg
     6001 gttcacttcg tatcagaaag ccactgaaga acatagtact cggcaaaaga gccctgaaat
     6061 acacaggaga attgacatct caccaagtac cctgaggaag catacccgtt tagcagggga
     6121 agagagagtt tttaaagaag aaaatcaaaa gggagataaa aaattaaggt gtgactctgc
     6181 tgaccttcgg catgacattg atcgccgtag aaaagaaaga agtaaagaac ggggagattc
     6241 caagggctcc agggaatcca gtggatcaag aaagcaggaa aaaactccaa aagattacaa
     6301 ggaatacaaa tcttacaaag atgacagtaa acataaaaga gagcaagatc attctcgatc
     6361 ttcatcctct tcagcatcac cttcttctcc cagttctcga gaagaaaagg agagtaagaa
     6421 ggaaagagaa gaagaattta aaactcacca tgaaatgaaa gaatactcag gctttgcagg
     6481 agttagccga ccacgaggaa ccttttttcg aattagaggc agaggaagag ccagaggagt
     6541 ttttgctggg acaaatactg gtccaaacaa ctcaaatact acttttcaaa agagaccgaa
     6601 ggaagaggaa tgggatccag aatatacccc aaagagcaag aagtacttct tgcatgacga
     6661 cagagatgat ggtgtggatt attgggccaa aagaggaaga ggtcgtggta cttttcaacg
     6721 tggcagaggg cgctttaact tcaaaaaatc aggtagcagt cctaaatgga ctcatgacaa
     6781 ataccaaggg gatgggattg ttgaagatga agaagagacc atggaaaata atgaagaaaa
     6841 gaaggacaga cgcaaggaag aaaaggaata gtaatgaact agtccgtaac ttgaaagtat
     6901 ttcgatttct tggctttata tatcttgtgg aaaggacgat ttaccgacgg atgattgaag
     6961 ttaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7381 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7501 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7561 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7621 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7681 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7741 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7801 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7861 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7921 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8101 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8221 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     8281 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8341 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8401 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8461 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8521 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8581 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8641 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8701 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8761 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8821 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8881 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9001 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9061 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9121 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9181 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9241 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9301 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9361 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9421 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9481 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9541 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9601 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9661 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9721 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9781 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9841 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9901 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9961 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10201 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10741 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10801 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10861 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11041 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    11101 aacaaaagct ggagctgcaa gc
//
