LOCUS       sequence-404565-       10876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1011
FEATURES             Location/Qualifiers
     source          1..10876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6733..7321
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6786..6806)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7204..7215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7393..7626
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7704..7825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7741..7760)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7795..7814
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7865..8000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8020..8039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8021..8039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8049..8066)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8049..8065)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8058..8080)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8207..8662
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8294..8313)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8504..8525
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8688..8792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8793..8861,8862..9653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9011..9030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9824..10412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10313..10332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10566..10583
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10700..10721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10736..10741,10742..10759,10760..10766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10774..10790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10779..10801
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10798..10814
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10798..10814
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10833..10853
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10835..10853
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcag gtggaccctc ctcttcacgg
     4141 gcctccaaat gactttctca tttttcaaat cattcctctg cactcacttt ctataatgcc
     4201 ccggtttctc tggattctgt gcttctccat ggaggaaact caaggagaac tgacaagttc
     4261 ttgtggttct aaaaccatgg ccaatgtatc tttggcattt agggatgtgt ccatagacct
     4321 ctcccaagag gagtgggagt gcctggacgc tgtgcagagg gacttgtaca aggatgtgat
     4381 gttggagaac tacagcaacc tggtctcact gggatatacc attcctaagc cagatgtgat
     4441 tactttattg gagcaagaga aagagccctg gatagtaatg agggaaggga caaggaattg
     4501 gttcacagat ttggaataca agtatattac caagaatttg ctttcagaaa agaatgtttg
     4561 caaaatctat ttatctcaat tgcagacagg ggaaaaaagt aaaaacacca tccatgagga
     4621 caccattttc agaaatggtt tgcagtgtaa acatgaattt gagagacaag agagacatca
     4681 gatgggatgc gttagtcaaa tgctaatcca aaaacaaata tctcatcctc tacatccaaa
     4741 aattcatgct agagagaaat catatgaatg taaggaatgt agaaaggcct ttagacaaca
     4801 gtcatacctt attcaacatc tgagaattca cactggtgag agaccctata aatgtatgga
     4861 atgtggaaag gccttttgtc gagtgggaga ccttagagta catcacacaa tccatgctgg
     4921 ggagagaccc tatgaatgta aagaatgtgg gaaggccttt agacttcatt atcaccttac
     4981 tgaacatcag agaatacatt ctggtgtgaa accctacgag tgtaaggaat gtgggaaagc
     5041 ctttagtcgt gttagagacc ttagagtaca tcagacaatt catgctggag agagacctta
     5101 tgaatgtaaa gaatgtggga aggcctttag acttcattat caactaactg aacatcaaag
     5161 aattcatact ggtgagaggc cttatgaatg taaggtttgt ggcaagacct ttagggtaca
     5221 acgacatatt agtcaacatc agaaaattca tactggtgtc aaaccctata aatgtaatga
     5281 atgtgggaag gcctttagtc atggctcata ccttgttcaa catcagaaaa ttcatactgg
     5341 tgaaaaaccc tacgaatgta aagaatgtgg taagtccttt agttttcatg cagaacttgc
     5401 tcgacatcgt agaattcata ctggtgagaa accctatgaa tgtagagaat gtggaaaagc
     5461 ctttcgtctt caaacggaac ttactcggca tcatagaact catactggtg agaaacccta
     5521 tgaatgtaag gaatgtggga aggcctttat ttgtggttat caacttactt tacatctgag
     5581 aactcacacc ggtgagattc cctatgaatg taaggaatgt ggaaaaacct tcagtagtcg
     5641 ctatcatctc actcaacact acagaattca tactggtgag aaaccctaca tatgtaacga
     5701 atgtggaaaa gcctttcgtc ttcaaggaga acttacccga catcacagaa ttcatacatg
     5761 tgagaaaccc tatgaatgta aggaatgtgg gaaggctttt attcatagca atcaatttat
     5821 ttcacaccag cgaattcaca ccagtgagag cacctacata tgtaaagaat gtgggaagat
     5881 ttttagtcgt cgctataatc ttactcaaca ttttaaaatt catactggtg aaaaacccta
     5941 catatgtaat gaatgtggga aagcctttcg atttcaaaca gaacttactc agcatcacag
     6001 aattcatact ggtgaaaaac cctataaatg tacagaatgt gggaaggcct ttattcgtag
     6061 cactcatctc acgcaacatc acagaattca tactggtgag aaaccctacg aatgtacgga
     6121 atgtgggaag acgtttagtc ggcactatca tcttactcaa catcacagag gccatactgg
     6181 tgagaagccc tacatatgta atgaatgtgg gaatgctttt atttgcagtt atcgacttac
     6241 attacatcaa agaattcaca ctggtgagct tccatatgaa tgtaaggaat gtggaaagac
     6301 ctttagtcgt cggtatcatc ttactcaaca ttttagactt catactggtg agaaacctta
     6361 tagctgtaaa gaatgtggga atgcctttcg tcttcaagca gaacttactc gacatcacat
     6421 agttcacacg ggtgagaaac cctataaatg taaagaatgt gggaaagcct tcagtgttaa
     6481 ttcagaactt actcgacatc acagaattca tactggtgaa aaaccctatc aatgtaaaga
     6541 atgtggaaaa gcctttattc gtagtgatca acttacttta catcagagaa atcatattag
     6601 tgaggaagtc ctatgcataa tgtagtaatg aactagtccg taacttgaaa gtatttcgat
     6661 ttcttggctt tatatatctt gtggaaagga cgaaaacgct actgaggacg ctccataacg
     6721 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6901 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6961 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7021 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7081 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7141 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7201 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7261 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7321 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7381 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7441 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7501 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7561 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7621 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7681 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7741 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7801 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7921 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7981 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     8041 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8101 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8161 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     8221 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8281 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8341 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8401 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8461 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8521 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8581 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8641 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9661 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9961 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10201 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10261 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10321 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10381 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10441 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10501 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10621 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    10681 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10741 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10801 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    10861 agctggagct gcaagc
//
