LOCUS       sequence-404599-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1204
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6346..6934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6399..6419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6817..6828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7006..7239
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7317..7438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7354..7373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7408..7427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7478..7613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7633..7652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7634..7652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7662..7679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7662..7678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7671..7693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7820..8275
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7907..7926)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8117..8138
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8301..8405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8406..8474,8475..9266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8624..8643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9437..10025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9926..9945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10179..10196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10313..10334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10349..10354,10355..10372,10373..10379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10387..10403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10392..10414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10411..10427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10411..10427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10446..10466
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10448..10466
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggga gaaaaaaatg gcgatgcaaa
     4141 aactttctgg atggagctag aagatgatgg aaaagtggac ttcatttttg aacaagtaca
     4201 aaatgtgctg cagtcactga aacaaaagat caaagatggg tctgccacca ataaagaata
     4261 catccaagca atgattctag tgaatgaagc aactataatt aacagttcaa catcaataaa
     4321 ggatcctatg cctgtgactc agaaggaaca ggaaaacaaa tccaatgcat ttccctctac
     4381 atcatgtgaa aactcctttc cagaagactg tacatttcta acaacagaaa ataaggaaat
     4441 tctctctctt gaagataaag ttgtagactt tagagaaaaa gactcatctt cgaatttatc
     4501 ttaccaaagt catgactgct ctggtgcttg tctgatgaaa atgccactga acttgaaggg
     4561 agaaaaccct ctgcagctgc caatcaaatg tcacttccaa agacgacatg caaagacaaa
     4621 ctctcattct tcagcactcc acgtgagtta taaaacccct tgtggaagga gtctacgaaa
     4681 cgtggaggaa gtttttcgtt acctgcttga gacagagtgt aactttttat ttacagataa
     4741 cttttctttc aatacctatg ttcagttggc tcggaattac ccaaagcaaa aagaagttgt
     4801 ttctgatgtg gatattagca atggagtgga atcagtgccc atttctttct gtaatgaaat
     4861 tgacagtaga aagctcccac agtttaagta cagaaagact gtgtggcctc gagcatataa
     4921 tctaaccaac ttttccagca tgtttactga ttcctgtgac tgctctgagg gctgcataga
     4981 cataacaaaa tgtgcatgtc ttcaactgac agcaaggaat gccaaaactt cccccttgtc
     5041 aagtgacaaa ataaccactg gatataaata taaaagacta cagagacaga ttcctactgg
     5101 catttatgaa tgcagccttt tgtgcaaatg taatcgacaa ttgtgtcaaa accgagttgt
     5161 ccaacatggt cctcaagtga ggttacaggt gttcaaaact gagcagaagg gatggggtgt
     5221 acgctgtcta gatgacattg acagagggac atttgtttgc atttattcag gaagattact
     5281 aagcagagct aacactgaaa aatcttatgg tattgatgaa aacgggagag atgagaatac
     5341 tatgaaaaat atattttcaa aaaagaggaa attagaagtt gcatgttcag attgtgaagt
     5401 tgaagttctc ccattaggat tggaaacaca tcctagaact gctaaaactg agaaatgtcc
     5461 accaaagttc agtaataatc ccaaggagct tactgtggaa acgaaatatg ataatatttc
     5521 aagaattcaa tatcattcag ttattagaga tcctgaatcc aagacagcca tttttcaaca
     5581 caatgggaaa aaaatggaat ttgtttcctc ggagtctgtc actccagaag ataatgatgg
     5641 atttaaacca ccccgagagc atctgaactc taaaaccaag ggagcacaaa aggactcaag
     5701 ttcaaaccat gttgatgagt ttgaagataa tctgctgatt gaatcagatg tgatagatat
     5761 aactaaatat agagaagaaa ctccaccaag gagcagatgt aaccaggcga ccacattgga
     5821 taatcagaat attaaaaagg caattgaggt tcaaattcag aaaccccaag agggacgatc
     5881 tacagcatgt caaagacagc aggtattttg tgatgaagag ttgctaagtg aaaccaagaa
     5941 tacttcatct gattctctaa caaagttcaa taaagggaat gtgtttttat tggatgccac
     6001 aaaagaagga aatgtcggcc gcttccttaa tcatagttgt tgcccaaatc tcttggtaca
     6061 gaatgttttt gtagaaacac acaacaggaa ttttccattg gtggcattct tcaccaacag
     6121 gtatgtgaaa gcaagaacag agctaacatg ggattatggc tatgaagctg ggactgtgcc
     6181 tgagaaggaa atcttctgcc aatgtggggt taataaatgt agaaaaaaaa tattatagta
     6241 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     6301 ggacgacaac tgatggagat gtcaatacag acgcgttaag tcgacaatca acctctggat
     6361 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6421 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6481 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6541 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6601 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6661 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6721 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6781 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6841 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6901 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     6961 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7021 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7081 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7141 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7201 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     7261 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7321 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7381 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7441 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7501 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7561 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     7621 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     7681 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7741 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7801 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7861 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7921 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7981 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8041 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8101 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8161 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8221 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     8281 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8341 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8401 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8461 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8521 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8581 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8641 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8701 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8761 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8821 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8881 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8941 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9001 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9061 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9121 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9181 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9241 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9301 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10081 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10141 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10201 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10261 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10321 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10381 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    10441 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
