LOCUS       sequence-404611-       12571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1350
FEATURES             Location/Qualifiers
     source          1..12571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8428..9016
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8481..8501)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8899..8910)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9088..9321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9399..9520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9436..9455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9490..9509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9560..9695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9715..9734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9716..9734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9744..9761)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9744..9760)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9753..9775)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9902..10357
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9989..10008)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10199..10220
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10383..10487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10488..10556,10557..11348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10706..10725)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11519..12107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12008..12027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12261..12278
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12395..12416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12431..12436,12437..12454,12455..12461)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12469..12485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12474..12496
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12493..12509
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12493..12509
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12528..12548
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12530..12548
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacaccgacc tgttcatgga
     4141 atgtgaggag gaggagttgg agccatggca gaaaatcagt gatgtcattg aggactctgt
     4201 agttgaagat tataattcag tggataaaac taccacagtt tctgtgagcc agcagccagt
     4261 ctcggctcca gtgcccatcg ctgcccatgc ttctgttgct gggcacctct ctacatccac
     4321 caccgttagt agcagcgggg cacagaacag cgacagtaca aagaagactc ttgtcacact
     4381 aattgccaac aacaatgctg gcaatccttt ggtccagcaa ggtggacagc cactcatcct
     4441 gacccagaat ccagccccag gtctgggcac aatggttact caaccagtat tgaggcctgt
     4501 tcaggtcatg cagaatgcca atcatgtgac tagttcccct gtggcctcac aaccaatatt
     4561 tatcactacg cagggatttc ctgtaaggaa tgtccggcct gtacaaaatg caatgaatca
     4621 ggttgggatt gtgctgaacg tacagcaagg ccaaacggtt agaccaatta cactagttcc
     4681 agccccaggt acccagtttg ttaagccgac agttggagtt ccacaagtgt tctcccagat
     4741 gacccctgtg aggccaggct ccacaatgcc tgtgaggccc accaccaaca ccttcaccac
     4801 cgtcatcccg gccactctta ccattcgaag caccgtccca cagtcccagt cccagcagac
     4861 caagtccact cccagcactt ctaccactcc cactgccaca cagccaacct cactggggca
     4921 actagctgtt cagtctccag gccagtcaaa ccagaccacg aatcccaagc tagtgagcat
     4981 tgccagcttt gtcactgtga agcgacctgg tgttacaggc gaaaatagca atgaagtggc
     5041 caaattggtg aataccctta acaccatccc ttccctgggc cagagtcctg ggccagtggt
     5101 ggtgtccaac aacagctctg ctcatggctc tcaaagaacc agcggacctg agtcttcaat
     5161 gaaagtgacc tcttccatcc cagtatttga cctccaggat ggtggacgga aaatatgtcc
     5221 acgatgtaat gctcaatttc gtgttactga agctttgaga ggtcacatgt gttactgttg
     5281 cccagaaatg gttgaatacc agaagaaagg aaagtccctg gattcagaac ccagtgtccc
     5341 atcagcagca aagcccccat cccctgagaa aacagctcct gttgcttcca caccctcttc
     5401 tacacctatt cctgctctgt caccgcctac caaagtacca gaaccaaatg agaacgtggg
     5461 cgatgccgtc cagaccaaac tcattatgct tgtagatgac ttctactatg gacgggatgg
     5521 tggcaaagta gcccagctca caaatttccc taaggtcgcc acatctttcc gatgcccaca
     5581 ttgtaccaaa aggctaaaaa acaatattcg attcatgaac catatgaaac accacgtaga
     5641 actcgatcag cagaacggtg aggtagatgg tcacactatc tgccagcact gttaccgcca
     5701 gttttccact cccttccagc ttcagtgcca cttggaaaat gttcatagtc cctatgaatc
     5761 tactaccaag tgcaagatct gtgaatgggc gtttgaaagt gagccactat ttctccagca
     5821 tatgaaggat actcataagc ctggagagat gccttatgtt tgccaggtgt gtcaatatcg
     5881 ctcctcactc tactctgagg tagatgtcca ttttcggatg atccatgagg atacccggca
     5941 tctgctctgc ccttattgcc tgaaggtctt caaaaatggc aatgcattcc aacagcatta
     6001 catgaggcac cagaagagaa atgtttatca ctgcaacaaa tgccggctgc agtttctctt
     6061 tgccaaggac aaaattgaac acaagcttca acaccataaa accttccgta aacccaagca
     6121 gctggagggc ttgaaaccag gcaccaaggt gacaatccgg gcttcccgag ggcagccacg
     6181 aactgttcct gtatcctcta atgatacacc tcccagcgcc ttgcaggagg cagcaccgct
     6241 gacctcctca atggaccctc tgcctgtctt cctttatccc cctgtccagc gcagcatcca
     6301 gaagagagct gttaggaaaa tgagtgtcat gggccggcag acatgcctgg agtgcagctt
     6361 cgagatccca gacttcccta atcatttccc tacttacgta cactgctctc tgtgtcgcta
     6421 tagcacctgc tgttctcgag cttatgccaa ccacatgatc aacaatcatg ttccacggaa
     6481 gagccccaag tatttggctt tgtttaaaaa ttctgtgagt ggaatcaagc tggcctgcac
     6541 ttcatgtacc tttgttacct ctgtgggcga tgctatggcc aagcatttgg tattcaaccc
     6601 ctctcacaga tccagcagca tcctgccacg gggactcact tggatagctc actcaaggca
     6661 tggccagact cgtgaccgag tgcatgaccg gaacgtgaag aatatgtacc ctcctccttc
     6721 cttccccact aacaaagctg ccactgtgaa atctgcgggg gccaccccag ctgagcctga
     6781 agagctacta actcccttag ccccagcact cccatcacca gcctcaactg caaccccacc
     6841 accaaccccc actcacccgc aggctttagc ccttccaccg ctggctacag agggagccga
     6901 atgtctgaat gttgatgatc aggatgaagg gagcccagtc acccaagaac ctgagctagc
     6961 atcaggtggt ggtggtagtg gtggagttgg caaaaaggag cagctgtctg tgaagaagct
     7021 tcgagtagta ctgtttgctc tatgctgcaa tacagaacag gcagctgaac acttccgaaa
     7081 tccccagcga cgtattcgcc gttggcttcg acgtttccag gcctcccagg gggagaatct
     7141 agagggcaaa tatctgagct ttgaggcaga agagaaactg gctgagtggg tgctaaccca
     7201 gcgcgaacaa cagctacctg taaatgagga gaccttgttc cagaaggcca ccaaaatagg
     7261 acgttctttg gaaggggggt ttaagatctc ctatgagtgg gctgtgcgtt tcatgctgcg
     7321 gcaccacctg actccccatg cccggcgagc tgtggcccac accctaccta aggatgtagc
     7381 agagaatgca ggactcttca ttgattttgt acaacggcag attcacaacc aggacttacc
     7441 cttgtctatg attgtggcta ttgatgagat ctctttgttc ctggatacag aggtgctgag
     7501 cagtgatgat cgaaaggaga atgccctgca gacagtgggc acaggggaac cttggtgtga
     7561 tgtagtccta gccattctgg cagatggcac tgtccttccc accctggttt tctacagagg
     7621 gcagatggat cagcctgcta acatgccaga ctccatattg ctagaggcaa aggagagtgg
     7681 ctacagtgat gacgagatca tggagctgtg gtcaactcga gtgtggcaga agcacacagc
     7741 ttgccagcgc agcaaaggca tgcttgtgat ggactgtcat cgcactcact tgtcagaaga
     7801 ggtactggct atgcttagtg cctctagcac tttgcctgca gtggtcccag caggctgtag
     7861 ctccaaaatt cagccattag atgtatgcat caaaagaact gtcaagaact tcctgcataa
     7921 aaaatggaag gaacaggctc gggaaatggc agatactgca tgtgattctg atgtcctgct
     7981 tcagctggtg cttgtctggc tgggtgaagt gctaggtgtc attggggact gtccagagct
     8041 agttcagcgc tccttcctgg tggctagtgt tctgcctggc cccgatggca acattaactc
     8101 acctacaaga aatgctgaca tgcaggagga gctaattgcc tccctagagg agcaactgaa
     8161 gctgagtggg gaacattctg agtcttccac tccacgaccc agatcatctc ctgaagagac
     8221 aattgagcct gaaagtcttc accagctctt tgagggtgaa agtgagaccg agtctttcta
     8281 tggctttgaa gaagctgacc tagatctgat ggagatttag taatgaacta gtccgtaact
     8341 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgact gggagctact
     8401 gcaaagccta ttacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8461 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8521 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8581 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8641 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8701 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8761 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8821 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8881 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8941 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9001 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     9061 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9121 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9181 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9241 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9301 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9361 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9541 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9601 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9661 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9721 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9781 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9841 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9901 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9961 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10021 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10081 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10141 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10201 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10261 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10321 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10381 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10441 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10501 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10561 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10621 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10681 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10741 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10801 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10861 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10921 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10981 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11041 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11101 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11161 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11221 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11281 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11341 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11641 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11941 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12181 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12241 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    12301 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12361 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12421 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12481 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12541 actaaaggga acaaaagctg gagctgcaag c
//
