LOCUS       sequence-404619-       12085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1531
FEATURES             Location/Qualifiers
     source          1..12085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7942..8530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7995..8015)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8413..8424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8602..8835
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8913..9034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8950..8969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9004..9023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9074..9209
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9229..9248)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9230..9248)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9258..9275)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9258..9274)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9267..9289)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9416..9871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9503..9522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9713..9734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9897..10001
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10002..10070,10071..10862)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10220..10239)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11033..11621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11522..11541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11775..11792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11909..11930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11945..11950,11951..11968,11969..11975)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11983..11999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11988..12010
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12007..12023
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12007..12023
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12042..12062
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12044..12062
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag ctccccaact acagccggca
     4141 gctgctgcag cagctgtaca ctctgtgcaa ggagcagcag ttctgtgatt gcaccatctc
     4201 cattggtacc atttacttca gggctcacaa gcttgtcctg gctgctgcca gcctcctgtt
     4261 caaaaccctg ctggataaca cagataccat ctccatcgat gcatctgtgg tgagccccga
     4321 ggagtttgcg ctcttgttgg aaatgatgta cacgggcaaa ctacctgtgg gcaagcacaa
     4381 cttctccaaa atcatctcct tagcagacag tctacagatg tttgatgtag ctgttagctg
     4441 caaaaatctt ctgaccagcc ttgtaaactg ctcggttcag ggtcaggtgg taagggatgt
     4501 ctctgcgcca tcctcagaga cattcagaaa ggaaccagag aagcctcaag tagaaatcct
     4561 ttcatctgaa ggtgctggag agcctcattc ttccccagag cttgctgcca ctccaggggg
     4621 ccctgtgaaa gctgagactg aggaagcagc ccattcagtt tcacaagaga tgagtgtgaa
     4681 ttctcccaca gcccaggaga gccagaggaa tgcagaaacc ccagcggaga ctcctactac
     4741 agctgaagct tgttccccct cccctgctgt gcaaaccttt agtgaggcaa agaagacaag
     4801 cacagaacca ggatgtgaaa ggaaacacta ccagctgaat tttcttctag aaaatgaagg
     4861 tgtcttctca gatgcactca tggttaccca ggatgtttta aaaaaactag aaatgtgttc
     4921 agaaattaaa ggtccacaga aggagatgat agtgaaatgt ttcgagggtg aaggaggaca
     4981 ttcagcattc cagagaatcc tgggtaaagt aagagaggaa agcctggatg ttcaaactgt
     5041 tgtgtccctg ttgaggctgt accaatattc taatcctgca gtaaaaacag cactattaga
     5101 caggaagcca gaagatgtag acacagtgca gccaaaaggg agcacagagg agggaaagac
     5161 cttgtctgtt ctgttactag aacacaaaga ggacctgata cagtgtgtaa cacagctgag
     5221 acctattatg gagtccctgg aaacagccaa ggaggaattc ctgactggca ctgaaaaaag
     5281 ggtgattctg aattgctgtg agggcagaac acccaaggag acaatagaaa atttgttgca
     5341 cagaatgact gaagagaaga cgctgactgc tgagggtttg gtaaaactcc tccaggctgt
     5401 gaagacgact ttcccaaacc tgggccttct gctagagaag ttgcagaaat cagccacttt
     5461 gccaagcacc acagtccaac caagccctga tgattatggg actgagctat tgagacgcta
     5521 tcatgaaaac ctctctgaga ttttcacaga caaccagatt ttattaaaga tgatctcaca
     5581 catgacaagt ttagcccctg gagaaagaga ggtcatggag aagcttgtga aacgtgactc
     5641 tggttcaggt ggtttcaatt ctctgatatc agcagttcta gaaaagcaga ctctctctgc
     5701 cacagccatt tggcaactgc tgctggtggt tcaggagaca aagacctgtc cattggacct
     5761 gctcatggag gaaatacgaa gggagcctgg tgccgatgct ttcttccggg cagtgaccac
     5821 cccagaacat gccactttag aaacaatcct gaggcataac cagttgatct tggaggccat
     5881 ccaacagaag attgagtaca agctctttac ctcggaggag gagcacctgg cagagactgt
     5941 gaaagagatt ctgagcattc cctctgagac agccagccct gaagcttccc tgagagcagt
     6001 gctgagcaga gccatggaaa aatcagtccc ggccattgaa atatgccacc tcctgtgcag
     6061 tgtccacaaa tcttttccag gcctgcagcc tgtcatgcag gagttggcat acattggtgt
     6121 ccttactaag gaagatggag agaaggaaac gtggaaggtg agtaataaat ttcaccttga
     6181 agccaacaac aaagaagatg aaaaggcagc caaagaagac agccagcctg gggaacagaa
     6241 tgatcaagga gagactggtt ccttgccagg acagcaagag aaagaggctt cagcctcccc
     6301 agaccctgcc aagaagagct tcatctgtaa ggcctgcgac aaaagcttcc atttctactg
     6361 ccgcctaaag gtgcacatga agcgctgccg ggtggctaag agcaaacagg tgcagtgtaa
     6421 ggagtgcagt gagaccaagg attcaaagaa agagctggac aaacatcagc tggaggccca
     6481 tggtgcaggt ggagagcccg atgcccccaa gaagaagaag aagaggcttc cagtgacatg
     6541 tgacctctgt ggcagagaat ttgcccatgc ctcaggcatg cagtaccata agctgacaga
     6601 gcacttcgat gagaagcctt tctcctgtga agagtgtggg gcgaagtttg cagccaattc
     6661 caccctgaag aaccaccttc gccttcacac cggggaccgc ccgttcatgt gcaagcactg
     6721 cctcatgacc ttcacccagg cctccgccct ggcctatcac accaagaaga agcactcaga
     6781 agggaaaatg tatgcatgcc agtactgtga tgctgtgttt gcccagtcta ttgagctgtc
     6841 ccgccacgtg aggacccaca ccggggacaa gccctatgtc tgcagagact gtggcaaggg
     6901 cttccggcaa gccaatggcc tctccatcca tctgcacacc tttcacaaca tagaagatcc
     6961 ttatgactgc aagaagtgca ggatgagttt ccccactctt caggatcacc ggaagcacat
     7021 ccatgaggtg cactccaaag agtaccaccc ctgccccacg tgtgggaaga tcttcagtgc
     7081 cccgtccatg ctggagcggc acgtggtgac ccacgttgga gggaagccct tcagctgcgg
     7141 gatctgcaac aaggcctacc agcaattgtc tggtttgtgg taccacaatc gaacccacca
     7201 ccctgacgta tttgctgctc agaaccaccg atcttccaag ttctcatcac tccagtgcag
     7261 ctcctgtgac aaaaccttcc ccaacaccat tgagcacaag aagcacatca aagcagaaca
     7321 tgcagatatg aagttccatg aatgtgacca gtgtaaggag ctcttcccca cgccagcctt
     7381 gctgcaggtt catgtcaagt gccagcattc agggtcccag ccattccggt gcttgtactg
     7441 tgctgctact ttccgttttc ctggagcatt gcagcaccat gtcaccacgg agcacttcaa
     7501 gcagtcagag accaccttcc cctgtgagct ctgtggggaa ctcttcacct cccaggccca
     7561 gcttgacagt cacctggaat ctgagcaccc aaaggtgatg agcacggaaa cccaggccgc
     7621 agcctcacag atggcgcagg tgatccaaac cccagagccg gtggccccga cagagcaggt
     7681 gatcactttg gaggagaccc agcttgccgg gtcgcaggtg tttgtgacgt tgccagattc
     7741 tcaggcatct caggccagct ctgagctcgt ggcggtgact gtggaggact tgctggatgg
     7801 cacagtgacg ctgatctgtg gtgaggccaa atagtaatga actagtccgt aacttgaaag
     7861 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaatttcaaa ctgctctgct
     7921 gatatcacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     7981 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8041 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8101 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8161 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8221 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8281 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8341 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8401 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8461 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8521 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     8581 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8641 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8701 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8761 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8821 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     8881 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9061 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9121 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9181 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9241 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9301 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9361 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     9421 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9481 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9541 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9601 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9661 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9721 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9781 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9841 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     9901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10861 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10921 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10981 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11161 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11821 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    11881 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    11941 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12001 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12061 gggaacaaaa gctggagctg caagc
//
