LOCUS       sequence-404689-       15009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synaptotagmin-1 sgRNA3 with TagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Joensuu M, Syed P, Saber SH, Lanoue V, Wallis TP, Rae J, Blum A, 
            Gormal RS, Small C, Sanders S, Jiang A, Mahrhold S, Krez N, Cousin 
            MA, Cooper-White R, Cooper-White JJ, Collins BM, Parton RG, 
            Balistreri G, Rummel A, Meunier FA
  TITLE     Presynaptic targeting of botulinum neurotoxin type A requires a 
            tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic 
            vesicle entry.
  JOURNAL   EMBO J. 2023 Jul 3;42(13):e112095. doi: 10.15252/embj.2022112095. 
            Epub 2023 May 25.
  PUBMED    37226896
REFERENCE   2  (bases 1 to 15009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-07-3"; volume: "42(13):e112095. doi"; pages: " 
            10.15252/embj.2022112095. Epub 2023 May 25"
COMMENT     Sequence Label: pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifi...
FEATURES             Location/Qualifiers
     source          1..15009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1241..1250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1250..1315
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1322..1342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1352..1378,1379..1381,1382..3865,3866..3871,3872..3937,
                     3938..3940,3941..5455)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1352..1378,1379..1381,1382..3868,3869..3871,
                     3872..5455)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1352..5455
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5468..5488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5522..5716
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5783..5803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5810..5863
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5864..6565
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(6619..6652)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6677..7265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6730..6750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7148..7159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7268..7284)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7269..7285)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7790..7970
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7996..8013)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8002..8226
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8272..8700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8359..8378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8569..8590
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8709..8729)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8714..9043
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8894..9029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8956..8975
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9091..9138
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9157..9531
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9661..9782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9698..9717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9752..9771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9831..9847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9831..9847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9844..9866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9855..9871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9879..9885,9886..9903,9904..9909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9924..9945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10062..10079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10233..10821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10313..10332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10992..11783,11784..11852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11615..11634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11853..11957)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12032..12051)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12223..12602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12604..12802
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12757..12777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             12820..13000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13047..13172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13665..13898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14083..14127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14425..14542
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(14586..14619)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(14630..14705)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(14729..14977)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(14787..14806)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(14957..14977)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga gccaccatgg actacaaaga
     1261 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagatggc
     1321 ccccaagaag aagaggaagg tgggccgcgg aatggacaag aagtactcca ttgggctcgc
     1381 catcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa
     1441 aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc
     1501 cctcctgttc gactccgggg aaaccgccga agccacgcgg ctcaaaagaa cagcacggcg
     1561 cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat
     1621 ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga
     1681 taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga
     1741 aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga
     1801 cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat
     1861 cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca
     1921 gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc
     1981 aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc
     2041 tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc
     2101 caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga gcaaagacac
     2161 ctacgatgat gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt
     2221 tttggcggca aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac
     2281 ggagatcacc aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca
     2341 agacttgact ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat
     2401 tttcttcgat cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga
     2461 ggaattttac aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct
     2521 ggtaaagctt aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat
     2581 cccccaccag attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta
     2641 cccctttttg aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta
     2701 ctatgtaggc cccctcgccc ggggaaattc cagattcgcg tggatgactc gcaaatcaga
     2761 agagaccatc actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc
     2821 cttcatcgaa aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa
     2881 acactctctg ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt
     2941 cacagaaggg atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga
     3001 cctcctcttc aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag actatttcaa
     3061 aaagattgaa tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc
     3121 cctgggaacg tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga
     3181 ggagaacgag gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga
     3241 gatgattgaa gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca
     3301 gctcaagagg cgccgatata caggatgggg gcggctgtca agaaaactga tcaatgggat
     3361 ccgagacaag cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa
     3421 ccggaacttc atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa
     3481 agcacaagtt tctggccagg gggacagtct tcacgagcac atcgctaatc ttgcaggtag
     3541 cccagctatc aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt
     3601 aatgggaagg cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac
     3661 ccagaaggga cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga
     3721 actggggtcc caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa
     3781 gctctacctg tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat
     3841 caatcggctc tccgactacg acgtggatgc catcgtgccc cagtcttttc tcaaagatga
     3901 ttctattgat aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt
     3961 cccctcagaa gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa
     4021 actgatcaca caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga
     4081 gttggataaa gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca
     4141 cgtggcccaa attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat
     4201 tcgagaggtg aaagttatta ctctgaagtc taagctggtc tcagatttca gaaaggactt
     4261 tcagttttat aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa
     4321 tgcagtggta ggcactgcac ttatcaaaaa atatcccaag cttgaatctg aatttgttta
     4381 cggagactat aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg
     4441 caaggccacc gctaagtact tcttttacag caatattatg aattttttca agaccgagat
     4501 tacactggcc aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg
     4561 agaaatcgtg tgggacaagg gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc
     4621 gcaggtgaac atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat
     4681 cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa
     4741 atacggcgga ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga
     4801 gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga
     4861 gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat ataaagaggt
     4921 caaaaaagac ctcatcatta agcttcccaa gtactctctc tttgagcttg aaaacggccg
     4981 gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc
     5041 taaatacgtt aatttcttgt atctggccag ccactatgaa aagctcaaag ggtctcccga
     5101 agataatgag cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat
     5161 cgagcaaata agcgaattct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt
     5221 gctttctgct tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat
     5281 ccacttgttt actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac
     5341 catagacaga aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca
     5401 gtcaattacg gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag
     5461 ggctgacccc aagaagaaga ggaaggtggc tagcgatgct aagtcactga ctgcctggtc
     5521 ccggacactg gtgaccttca aggatgtgtt tgtggacttc accagggagg agtggaagct
     5581 gctggacact gctcagcaga tcctgtacag aaatgtgatg ctggagaact ataagaacct
     5641 ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg agaagggaga
     5701 agagccctgg ctggtggaga gagaaattca ccaagagacc catcctgatt cagagactgc
     5761 atttgaaatc aaatcatcag ttccgaaaaa gaaacgcaaa gttgctagcg agggcagagg
     5821 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgagcg agctgattaa
     5881 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     5941 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     6001 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     6061 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     6121 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     6181 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     6241 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgaaac
     6301 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     6361 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
     6421 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     6481 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     6541 caaactgggg cacaagctta attgaccagc acactggcgg ccgttactag cttctgcagc
     6601 acgaccggtt gataatagat aacttcgtat agcatacatt atacgaagtt atgaattcga
     6661 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6721 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6781 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6841 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6901 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6961 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7021 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7081 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7141 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7201 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7261 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     7321 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7381 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7441 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7501 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     7561 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     7621 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     7681 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     7741 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7921 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     7981 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     8041 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     8101 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     8161 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     8221 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     8281 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8341 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8401 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8461 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8521 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8581 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8641 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8701 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8761 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8881 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     9001 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     9061 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     9121 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     9181 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     9241 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     9301 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     9361 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     9421 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     9481 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     9541 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9601 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     9661 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9721 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9781 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9901 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9961 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    10021 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10081 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10141 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10201 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10261 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10321 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10381 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10441 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10501 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10561 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10621 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10681 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10801 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10861 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10921 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10981 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    11041 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11101 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11161 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11221 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11281 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11341 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11401 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11461 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11521 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11581 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11641 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11701 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11761 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11821 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11881 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11941 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    12001 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    12061 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
    12121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
    12181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
    12241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
    12301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
    12361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
    12421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
    12481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
    12541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
    12601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
    12661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
    12721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
    12781 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
    12841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    12901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    12961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
    13021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    13081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    13141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    13201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    13261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    13321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    13381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    13441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    13501 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    13561 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    13621 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    13681 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    13741 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    13801 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    13861 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    13921 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    13981 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    14041 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    14101 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    14161 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    14221 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    14281 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    14341 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
    14401 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
    14461 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    14521 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
    14581 aattaataac ttcgtatagc atacattata cgaagttatt aaaaaaaaag caccgactcg
    14641 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
    14701 aaaacccgat gctgccgctg caggaggagg tgtttcgtcc tttccacaag atatataaag
    14761 ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata
    14821 attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta
    14881 atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc ttgtatcgta
    14941 tatgcaaata tgaaggaatc atgggaaata ggccctctta attaattaac ccgtgtcggc
    15001 tccagatct
//
