LOCUS       sequence-404690-       15229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Viral silencing plasmid targeting the CHLH gene from C. roseus. For 
            use as a positive control for VIGS experiments..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 15229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN381 - pTRV2-CHLH
FEATURES             Location/Qualifiers
     source          1..15229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(361..377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(361..377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(374..396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    385..401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(409..415,416..433,434..439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    454..475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(1024..1276)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1666..2460)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1777..1796)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2387..2406
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2481..2664)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(2517..2696)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2820..2844
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3309..3959
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(4147..4164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4398..4417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            4640..4749
                     /note="incP origin of transfer"
     CDS             4782..5153
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      5280..5991
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  6614..7381
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             7573..8367
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             8666..9814
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11410..11434
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(11681..11702)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11893..11912
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12037..12059
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12051..12068
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12052..12068
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12599..12623
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(13123..13147)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(14810..15155)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(14847..14867)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gaccatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
       61 tgttcctcgt gggtgggggt ccatctttgg gaccactgtc ggtagaggca tcttgaacga
      121 tagcctttcc tttatcgcaa tgatggcatt tgtagaagcc atcttccttt tctactgtcc
      181 tttcgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
      241 tttgttgaaa agtctcaata gccctctggt cttctgagac tgtatctttg atattcttgg
      301 agtagacgag agtgtcgtgc tccaccatgt tgacctgcag ccaagcttgg cgtaatcatg
      361 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      421 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      481 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      541 cggccaacgc gcggggagag gcggtttgcg tattgggcca aagacaaaag ggcgacattc
      601 aaccgattga gggagggaag gtaaatattg acggaaatta ttcattaaag gtgaattatc
      661 accgtcaccg acttgagcca tttgggaatt agagccagca aaatcaccag tagcaccatt
      721 accattagca aggccggaaa cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt
      781 agcgacagaa tcaagtttgc ctttagcgtc agactgtagc gcgttttcat cggcattttc
      841 ggtcatagcc cccttattag cgtttgccat cttttcataa tcaaaatcac cggaaccaga
      901 gccaccaccg gaaccgcctc cctcagagcc gccaccctca gaaccgccac cctcagagcc
      961 accaccctca gagccgccac cagaaccacc accagagccg ccgccagcat tgacaggagg
     1021 cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
     1081 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
     1141 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
     1201 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
     1261 aaatgtttga acgatcgggg atcatccggg tctgtggcgg gaactccacg aaaatatccg
     1321 aacgcagcaa gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt
     1381 tgatgtggac gccgggcccg atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg
     1441 ccgttgctgt cgtaatgata tcggcacctt cgaccgcctg ttccgcagag atcccgtggg
     1501 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     1561 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     1621 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     1681 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     1741 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     1801 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     1861 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc
     1921 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     1981 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     2041 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     2101 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     2161 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     2221 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     2281 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     2341 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     2401 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     2461 gcgaaacgat ccagatccgg tgcagattat ttggattgag agtgaatatg agactctaat
     2521 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     2581 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     2641 tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
     2701 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     2761 taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
     2821 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     2881 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     2941 ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg cccgaaacga
     3001 tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata cagcgccagc
     3061 agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc
     3121 agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg
     3181 atcaggggta tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga
     3241 acgcgcggat tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag
     3301 tgtcaagcat gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga
     3361 acgaggtcgg cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc
     3421 agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag
     3481 ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat
     3541 ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc
     3601 gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc
     3661 gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca
     3721 gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc
     3781 gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct
     3841 tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat
     3901 tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gacgattgat
     3961 caggaccgct gccggagcgc aacccactca ctacagcaga gccatgtaga caacatcccc
     4021 tccccctttc caccgcgtca gacgcccgta gcagcccgct acgggctttt tcatgccctg
     4081 ccctagcgtc caagcctcac ggccgcgctc ggcctctctg gcggccttct ggcgctcttc
     4141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4501 ggcgcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     4561 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     4621 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     4681 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     4741 gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc
     4801 caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga
     4861 ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc
     4921 agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca
     4981 atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca
     5041 cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg
     5101 acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt
     5161 agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc
     5221 aagaagagcg acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgag
     5281 cgcctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg
     5341 ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg
     5401 ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac
     5461 ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc
     5521 ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt
     5581 cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg
     5641 cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag
     5701 atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg
     5761 caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc
     5821 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
     5881 gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc
     5941 cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcag
     6001 tccttgccat tgccgggatc ggggcagtaa cgggatgggc gatcagcccg agcgcgacgc
     6061 ccggaagcat tgacgtgccg caggtgctgg catcgacatt cagcgaccag gtgccgggca
     6121 gtgagggcgg cggcctgggt ggcggcctgc ccttcacttc ggccgtcggg gcattcacgg
     6181 acttcatggc ggggccggca atttttacct tgggcattct tggcatagtg gtcgcgggtg
     6241 ccgtgctcgt gttcgggggt gcgataaacc cagcgaacca tttgaggtga taggtaagat
     6301 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     6361 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     6421 tattgacaat actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg
     6481 atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc
     6541 ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat
     6601 tctatcataa ttgggtaatg actccaactt attgatagtg ttttatgttc agataatgcc
     6661 cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc
     6721 gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc
     6781 tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc
     6841 atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc gccatagtgc
     6901 gttcaccgaa tacgtgcgca acaaccgtct tccggagact gtcatacgcg taaaacagcc
     6961 agcgctggcg cgatttagcc ccgacatagc cccactgttc gtccatttcc gcgcagacga
     7021 tgacgtcact gcccggctgt atgcgcgagg ttaccgactg cggcctgagt tttttaagtg
     7081 acgtaaaatc gtgttgaggc caacgcccat aatgcgggct gttgcccggc atccaacgcc
     7141 attcatggcc atatcaatga ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa
     7201 ctgcagttgc catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcggtgct
     7261 tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga
     7321 agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac
     7381 ccataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
     7441 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
     7501 ggagttcgtc ttgttataat tagcttcttg ggggatcttt aaatactgta gaaaagagga
     7561 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
     7621 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
     7681 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
     7741 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
     7801 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
     7861 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
     7921 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
     7981 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
     8041 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
     8101 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
     8161 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
     8221 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag
     8281 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
     8341 tatattttac tggatgaatt gttttagtac ctagatgtgg cgcaacgatg ccggcgacaa
     8401 gcaggagcgc accgacttct tccgcatcaa gtgttttggc tctcaggccg aggcccacgg
     8461 caagtatttg ggcaaggggt cgctggtatt cgtgcagggc aagattcgga ataccaagta
     8521 cgagaaggac ggccagacgg tctacgggac cgacttcatt gccgataagg tggattatct
     8581 ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg cacattgccc cggcgtgagt
     8641 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
     8701 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
     8761 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
     8821 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
     8881 cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat
     8941 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
     9001 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
     9061 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
     9121 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
     9181 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
     9241 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
     9301 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
     9361 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
     9421 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
     9481 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
     9541 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
     9601 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
     9661 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
     9721 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
     9781 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
     9841 gggttcagca gccagcgctt tactggcatt tcaggaacaa gcgggcactg ctcgacgcac
     9901 ttgcttcgct cagtatcgct cgggacgcac ggcgcgctct acgaactgcc gataaacaga
     9961 ggattaaaat tgacaattgt gattaaggct cagattcgac ggcttggagc ggccgacgtg
    10021 caggatttcc gcgagatccg attgtcggcc ctgaagaaag ctccagagat gttcgggtcc
    10081 gtttacgagc acgaggagaa aaagcccatg gaggcgttcg ctgaacggtt gcgagatgcc
    10141 gtggcattcg gcgcctacat cgacggcgag atcattgggc tgtcggtctt caaacaggag
    10201 gacggcccca aggacgctca caaggcgcat ctgtccggcg ttttcgtgga gcccgaacag
    10261 cgaggccgag gggtcgccgg tatgctgctg cgggcgttgc cggcgggttt attgctcgtg
    10321 atgatcgtcc gacagattcc aacgggaatc tggtggatgc gcatcttcat cctcggcgca
    10381 cttaatattt cgctattctg gagcttgttg tttatttcgg tctaccgcct gccgggcggg
    10441 gtcgcggcga cggtaggcgc tgtgcagccg ctgatggtcg tgttcatctc tgccgctctg
    10501 ctaggtagcc cgatacgatt gatggcggtc ctgggggcta tttgcggaac tgcgggcgtg
    10561 gcgctgttgg tgttgacacc aaacgcagcg ctagatcctg tcggcgtcgc agcgggcctg
    10621 gcgggggcgg tttccatggc gttcggaacc gtgctgaccc gcaagtggca acctcccgtg
    10681 cctctgctca cctttaccgc ctggcaactg gcggccggag gacttctgct cgttccagta
    10741 gctttagtgt ttgatccgcc aatcccgatg cctacaggaa ccaatgttct cggcctggcg
    10801 tggctcggcc tgatcggagc gggtttaacc tacttccttt ggttccgggg gatctcgcga
    10861 ctcgaaccta cagttgtttc cttactgggc tttctcagcc ccagatctgg ggtcgatcag
    10921 ccggggatgc atcaggccga cagtcggaac ttcgggtccc cgacctgtac cattcggtga
    10981 gcaatggata ggggagttga tatcgtcaac gttcacttct aaagaaatag cgccactcag
    11041 cttcctcagc ggctttatcc agcgatttcc tattatgtcg gcatagttct caagatcgac
    11101 agcctgtcac ggttaagcga gaaatgaata agaaggctga taattcggat ctctgcgagg
    11161 gagatgatat ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    11221 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    11281 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    11341 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    11401 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
    11461 cacattgcgg acgtttttaa tgtactgggg tggtttttct tttcaccagt gagacgggca
    11521 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
    11581 tttgccccag caggcgaaaa tcctgtttga tggtggttcc gaaatcggca aaatccctta
    11641 taaatcaaaa gaatagcccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    11701 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    11761 cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    11821 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    11881 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgcc attcaggctg cgcaactgtt
    11941 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    12001 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    12061 cggccagtga attcgaggtg gtggtggtgg tggctagcgt tctcccgttt cgtcctttag
    12121 ggactcgtca gtgtactgat ataagtacag acgggcgtaa taacgcttac gtaggcgagg
    12181 ggttttaccc cttttgcctt tgtaaccatc atcactttaa aagtacttac tcacccccca
    12241 acaatctctt agccgcgagg ctttttcaga tcgttgagaa ttttaataaa atacaaataa
    12301 aataacaaca caaacaaagt ccgttcccct atggtaagac aatgagtcgg ccaaacgccg
    12361 atctcaaaca gtctatacac agaaacagat aacaatttaa aataaaatca aaaagaaaat
    12421 taaattacaa aagacttacc gatcaatcaa gatcagtcga gaatgtcaat ctcgtaggtt
    12481 taaattgaac ctaaaacttc agacacggat ctacttaaag aaccgtagtt taatgtcttc
    12541 gggacatgcc cgggcctcga gacgcgtgag ctcggtaccg gatccccatg gaggatcaac
    12601 aagtttgtac aaaaaagcag gctcagttgc cacactagtt aatattgctg cattggaccg
    12661 tcctgaagat gggatttcct cacttcctgg aatattggct cagacagttg gaagggagat
    12721 tgagaatgtc tacaggggaa gtgacaaggg catactacga gatgtggaac tgctccggca
    12781 aataactgag gcatcgcgcg gagcaattac tgcatttgtt gaacgaacta ctaacagcaa
    12841 gggtcaagtt gttgatgttt ctgacaagtt gacttcaatt cttggttttg gcataaacga
    12901 accatgggtt cagtacttgt caaataccaa attctacaga gctgacaggg ataaacttag
    12961 aattttattc caattcttag gggagtgcct gaagcttgtt gtagctgata acgagttggg
    13021 aagtttgaaa caggctttgg aaggtaaata tgtggaacca ggaccgggag gagacccaat
    13081 cagaaaccca aaagttttgc caacgggaaa gaatatccat gcacccagct ttcttgtaca
    13141 aagtggttga tccttctaga gaattcggta accttactca cagaatctaa gtccactcgt
    13201 ccgtaataat aaagctcatc gtgcttcctt gagtaacaat gtccatcttt gttgacacac
    13261 aagaaccgcc tgaacataaa aacaaatacg ttctctactt ttccctcaaa cagcgtatca
    13321 tctcccagtg aattatcaaa aactgacaac tttccaatta cttcatacgt gcgaatatgt
    13381 gggactctac tacgtacctt gaccaccaaa ccattatcta acaataacac attagtgtcg
    13441 tcaagccact tcctaagtaa ttcgtgcatt gcgaaactca aatgctacca acgacactga
    13501 cgtcaatggt aaaggaaatt ccctcaaatt agtcacaatt agccctattt agatgtcgct
    13561 aatggcatca gtgaatatgg tatcacccac cctctgaatt tcacagagat ctcaacagag
    13621 ttcacgtcct taaatcccta gggattagga cgtatcggac ctccactcgc tggaggcggt
    13681 gcagatgaac tagcagctgc tccaccaaac ttaatcccga atactttctc aaagttcctt
    13741 cggtaataac tcttcaactc catgttctct aacgaagtta gagatttgtg gaaagctgta
    13801 gaggcatcat cgaatttgta ataagtgtac aagtcttctt ccaaagtcga gccagtgttc
    13861 gccttggtag tagtagtact cccttggttc gtcgtaaccg ttgtgtttgg attcgcagga
    13921 atatttgtaa cacccgccac tgcattcttc tcatcagctc gtctcttgaa cgctgaagcg
    13981 ttctgaatca caccaagagc actgttagcc cgtgagtcta aaaggaaata caccttctta
    14041 tctggaaact gctcatatgg tgaataagga cactgtctat tcagtgccgc tagtaaccca
    14101 gtgatctcat ctctaccaga ctgtagtgca aattttatgc tgtgtaactt cttcaacaga
    14161 tctttccacg aaactgattc cacccaagaa tcgtccatca agtcagcagg accgcccgac
    14221 ttgtcaaatg attcatcgta catatctccc atgttgatta gcgcaagtga ttcagtaacc
    14281 tgtagttcaa caagtctaat cgtccaaatt aaaatgatct tatcagcaga cacaagtatt
    14341 atatttatgc catctctaat gataacatta aagacttagc tagttttctc taaagaaact
    14401 agcaaaaccc caacgtccgt ttagacgctt gcgtaggaga agttaattaa aacgacccca
    14461 tcgtttttgc ggtagcgatt aaaaccacca cctttctttc attgctactt tcgtaagcga
    14521 tacgtctcag gttttccttt gtatgagaag acaagtgctt tcttactaac ctaaagaacc
    14581 gaaacccaaa gtaacatttc aaagataagc acaccagtca ctttcccgta cggtactaag
    14641 ctggatagtt caaaaagaaa tattacactg acaaaaacct cgtcagggag agggataaag
    14701 gctgtgttgg ataaggacgt tctaccgtat tctccaactt gtcaattagt acattctact
    14761 gcgatcactg acatacccca gccagggcaa caccataggt gcaatgtttt atcctctcca
    14821 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
    14881 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
    14941 cgtcttcttt ttccacgatg ttcctcgtgg gtgggggtcc atctttggga ccactgtcgg
    15001 tagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg tagaagccat
    15061 cttccttttc tactgtcctt tcgatgaagt gacagatagc tgggcaatgg aatccgagga
    15121 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctctggtct tctgagactg
    15181 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtt
//
