LOCUS       sequence-404705-        8929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the LbCas12a nuclease variant ""LbCas12a-Plus"" that 
            enhanced the editing activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Huang H, Huang G, Tan Z, Hu Y, Shan L, Zhou J, Zhang X, Ma S, Lv W, 
            Huang T, Liu Y, Wang D, Zhao X, Lin Y, Rong Z
  TITLE     Engineered Cas12a-Plus nuclease enables gene editing with enhanced 
            activity and specificity.
  JOURNAL   BMC Biol. 2022 Apr 25;20(1):91. doi: 10.1186/s12915-022-01296-1.
  PUBMED    35468792
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-04-25"; volume: "20(1):91. doi"; pages: " 
            10.1186/s12915-022-01296-1"
COMMENT     Sequence Label: pCAG-LbCas12a-Plus-mCherry
FEATURES             Location/Qualifiers
     source          1..8929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        11..390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..667
                     /label=chicken beta-actin promoter
     intron          668..1684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1608..1630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1692..1711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1739..1756
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1758..1777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1758..1776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1778..5461
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKEKFEAAQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5462..5509
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5516..5596
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5621..5677
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5684..6394
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5829..5847)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6097..6116
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6437..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6474..6493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6528..6547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6607..6623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6607..6623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6620..6642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6631..6647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6655..6661,6662..6679,6680..6685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6700..6721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6838..6855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7009..7597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7089..7108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7768..8559,8560..8628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8391..8410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8629..8733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8812..8831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattcgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
       61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gggcgcgccr ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1441 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1501 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1561 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1621 gcgtgtgacc ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcat
     1741 ttaggtgaca ctatagataa tacgactcac tatagggatg agcaagctgg agaagtttac
     1801 aaactgctac tccctgtcta agaccctgag gttcaaggcc atccctgtgg gcaagaccca
     1861 ggagaacatc gacaataagc ggctgctggt ggaggacgag aagagagccg aggattataa
     1921 gggcgtgaag aagctgctgg atcgctacta tctgtctttt atcaacgacg tgctgcacag
     1981 catcaagctg aagaatctga acaattacat cagcctgttc cggaagaaaa ccagaaccga
     2041 gaaggagaat aaggagctgg agaacctgga gatcaatctg cggaaggaga tcgccaaggc
     2101 cttcaagggc aacgagggct acaagtccct gtttaagaag gatatcatcg agacaatcct
     2161 gccagagttc ctggacgata aggacgagat cgccctggtg aacagcttca atggctttac
     2221 cacagccttc accggcttct ttagaaacag agagaatatg ttttccgagg aggccaagag
     2281 cacatccatc gccttcaggt gtatcaacga gaatctgacc cgctacatct ctaatatgga
     2341 catcttcgag aaggtggacg ccatctttga taagcacgag gtgcaggaga tcaaggagaa
     2401 gatcctgaac agcgactatg atgtggagga tttctttgag ggcgagttct ttaactttgt
     2461 gctgacacag gagggcatcg acgtgtataa cgccatcatc ggcggcttcg tgaccgagag
     2521 cggcgagaag atcaagggcc tgaacgagta catcaacctg tataatcaga aaaccaagca
     2581 gaagctgcct aagtttaagc cactgtataa gcaggtgctg agcgatcggg agtctctgag
     2641 cttctacggc gagggctata catccgatga ggaggtgctg gaggtgttta gaaacaccct
     2701 gaacaagaac agcgagatct tcagctccat caagaagctg gagaagctgt tcaagaattt
     2761 tgacgagtac tctagcgccg gcatctttgt gaagaacggc cccgccatca gcacaatctc
     2821 caaggatatc ttcggcgagt ggaacgtgat ccgggacaag tggaatgccg agtatgacga
     2881 tatccacctg aagaagaagg ccgtggtgac cgagaagtac gaggacgatc ggagaaagtc
     2941 cttcaagaag atcggctcct tttctctgga gcagctgcag gagtacgccg acgccgatct
     3001 gtctgtggtg gagaagctga aggagatcat catccagaag gtggatgaga tctacaaggt
     3061 gtatggctcc tctgagaagc tgttcgacgc cgattttgtg ctggagaaga gcctgaagaa
     3121 gaacgacgcc gtggtggcca tcatgaagga cctgctggat tctgtgaaga gcttcgagaa
     3181 ttacatcaag gccttctttg gcgagggcaa ggagacaaac agggacgagt ccttctatgg
     3241 cgattttgtg ctggcctacg acatcctgct gaaggtggac cacatctacg atgccatccg
     3301 caattatgtg acccagaagc cctactctaa ggataagttc aagctgtatt ttcagaaccc
     3361 tcagttcatg ggcggctggg acaaggataa ggagacagac tatcgggcca ccatcctgag
     3421 atacggctcc aagtactatc tggccatcat ggataagaag tacgccaagt gcctgcagaa
     3481 gatcgacaag gacgatgtga acggcaatta cgagaagatc aactataagc tgctgcccgg
     3541 ccctaataag atgctgccaa aggtgttctt ttctaagaag tggatggcct actataaccc
     3601 cagcgaggac atccagaaga tctacaagaa tggcacattc aagaagggcg atatgtttaa
     3661 cctgaatgac tgtcacaagc tgatcgactt ctttaaggat agcatctccc ggtatccaaa
     3721 gtggtccaat gcctacgatt tcaacttttc tgagacagag aagtataagg acatcgccgg
     3781 cttttacaga gaggtggagg agcagggcta taaggtgagc ttcgagtctg ccagcaagaa
     3841 ggaggtggat aagctggtgg aggagggcaa gctgtatatg ttccagatct ataacaagga
     3901 cttttccgat aagtctcacg gcacacccaa tctgcacacc atgtacttca agctgctgtt
     3961 tgacgagaac aatcacggac agatcaggct gagcggagga gcagagctgt tcatgaggcg
     4021 cgcctccctg aagaaggagg agctggtggt gcacccagcc aactccccta tcgccaacaa
     4081 gaatccagat aatcccaaga aaaccacaac cctgtcctac gacgtgtata aggataagag
     4141 gttttctgag gaccagtacg agctgcacat cccaatcgcc atcaataagt gccccaagaa
     4201 catcttcaag atcaatacag aggtgcgcgt gctgctgaag cacgacgata acccctatgt
     4261 gatcggcatc gataggggcg agcgcaatct gctgtatatc gtggtggtgg acggcaaggg
     4321 caacatcgtg gagcagtatt ccctgaacga gatcatcaac aacttcaacg gcatcaggat
     4381 caagacagat taccactctc tgctggacaa gaaggagaag gagaagttcg aggccgccca
     4441 gaactggacc tccatcgaga atatcaagga gctgaaggcc ggctatatct ctcaggtggt
     4501 gcacaagatc tgcgagctgg tggagaagta cgatgccgtg atcgccctgg aggacctgaa
     4561 ctctggcttt aagaatagcc gcgtgaaggt ggagaagcag gtgtatcaga agttcgagaa
     4621 gatgctgatc gataagctga actacatggt ggacaagaag tctaatcctt gtgcaacagg
     4681 cggcgccctg aagggctatc agatcaccaa taagttcgag agctttaagt ccatgtctac
     4741 ccagaacggc ttcatctttt acatccctgc ctggctgaca tccaagatcg atccatctac
     4801 cggctttgtg aacctgctga aaaccaagta taccagcatc gccgattcca agaagttcat
     4861 cagctccttt gacaggatca tgtacgtgcc cgaggaggat ctgttcgagt ttgccctgga
     4921 ctataagaac ttctctcgca cagacgccga ttacatcaag aagtggaagc tgtactccta
     4981 cggcaaccgg atcagaatct tccggaatcc taagaagaac aacgtgttcg actgggagga
     5041 ggtgtgcctg accagcgcct ataaggagct gttcaacaag tacggcatca attatcagca
     5101 gggcgatatc agagccctgc tgtgcgagca gtccgacaag gccttctact ctagctttat
     5161 ggccctgatg agcctgatgc tgcagatgcg gaacagcatc acaggccgca ccgacgtgga
     5221 ttttctgatc agccctgtga agaactccga cggcatcttc tacgatagcc ggaactatga
     5281 ggcccaggag aatgccatcc tgccaaagaa cgccgacgcc aatggcgcct ataacatcgc
     5341 cagaaaggtg ctgtgggcca tcggccagtt caagaaggcc gaggacgaga agctggataa
     5401 ggtgaagatc gccatctcta acaaggagtg gctggagtac gcccagacca gcgtgaagca
     5461 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc
     5521 atacgatgtt ccagattacg cttatcccta cgacgtgcct gattatgcat acccatacga
     5581 tgtccccgac tatgccctcg agagcaccgg tggcagcgga gctactaact tcagcctgct
     5641 gaagcaggct ggagacgtgg aggagaaccc tggacctgcc ggtatggtga gcaagggcga
     5701 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     5761 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5821 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     5881 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     5941 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6001 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6061 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     6121 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     6181 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     6241 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     6301 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6361 ctccaccggc ggcatggacg agctgtacaa gtaactgcag cgcggggatc tcatgctgga
     6421 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6541 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6601 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6661 acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt
     6721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7441 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8761 cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg
     8821 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     8881 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgac
//
