LOCUS       sequence-404752-        1845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the ampicillin resistance gene insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo7
FEATURES             Location/Qualifiers
     source          1..1845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      209..236
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(257..1048,1049..1117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     880..899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1118..1222)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            1419..1528
                     /note="incP origin of transfer"
     rep_origin      join(1643..1845,1..186)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa aaaagaatat
       61 ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag ggatgtaacg
      121 cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa cacttaacgg
      181 ctgacatggg ggtctcgctc ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      241 tacctaggag tcaaaattac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
      301 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
      361 gcttaccatc tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag
      421 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
      481 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
      541 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
      601 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
      661 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
      721 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
      781 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
      841 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
      901 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
      961 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     1021 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     1081 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     1141 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1201 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgag cgcgagaccg
     1261 tcaattcgag ggggatcaat tccgttttcc gctgcataac cctgcttcgg ggtcattata
     1321 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     1381 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     1441 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     1501 gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg
     1561 atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcacgga attgatccgg
     1621 ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt
     1681 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
     1741 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
     1801 catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaat
//
