LOCUS       sequence-404753-        2071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the ColE1 origin insert with an 
            ampicillin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo3'
FEATURES             Location/Qualifiers
     source          1..2071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     515..534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(753..857)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1062..1650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1551..1570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1804..1821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      1915..1942
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1963..2071,1..683,684..752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
       61 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      121 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      181 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      241 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      301 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      361 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      421 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      481 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      541 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      601 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      661 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      721 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      781 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      841 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtctcg agcggctcgc
      901 cagtcgattg gctgagctca taagttccta ttccgaagtt ccgcgaacgc gtaaaggatc
      961 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctgg tagcggtggt ttgtttgccg
     1141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1201 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     1861 catctgtgcg gtatttcaca ccgcatatgc tggatcgaga cgagacctca cactggctca
     1921 ccttcgggtg ggcctttctg cgtttatacc taggagtcaa aattaccaat gcttaatcag
     1981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2041 cgtgtagata actacgatac gggagggctt a
//
