LOCUS       sequence-404755-        2637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the erythromycin resistance gene insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo10
FEATURES             Location/Qualifiers
     source          1..2637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            1808..1917
                     /note="incP origin of transfer"
     rep_origin      2105..2493
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      2516..2543
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tgattttcct gattctattc gtgaataatt tgatctactc atttctaatt cttgggagaa
       61 tttttctttt tatttggtac ctctcatctt ttttaagctt tctctaaatg ctagttccaa
      121 gtttctcccc cctctttttg tgtaataaaa ttcacgcttt atttaaatta tagcttttaa
      181 tatcttaata aacaagtgat taatcacttg tgatttgtga ttaatcactt gtgatttgtg
      241 attattccaa aagtaggtaa tttcaccaac ctttttcaaa agtttttatc gacgattatt
      301 ggactggatc gtgtttccta aagcgattct aagcgctagg gacctcttta gctccttgga
      361 agctgtcagt agtataccta ataatttatc tacattccct ttagtaacgt gtaactttcc
      421 aaatttacaa aagcgactca tagaattatt tcctcccgtt aaataataga taactattaa
      481 aaatagacaa tacttgctca taagtaacgg tacttaaatt gtttactttg gcgtgtttca
      541 ttgcttgatg aaactgattt ttagtaaaca gttgacgata ttctcgattg acccattttg
      601 aaacaaagta cgtatatagc ttccaatatt tatctggaac atctgtggta tggcgggtaa
      661 gttttattaa gacactgttt acttttggtt taggatgaaa gcattccgct ggcagcttaa
      721 gcaattgctg aatcgagact tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca
      781 aggtacgctt gtagaatcct tcttcaacaa tcagatagat gtcagacgca tggctttcaa
      841 aaaccacttt tttaataatt tgtgtgctta aatggtaagg aatactccca acaattttat
      901 acctctgttt gttagggaat tgaaactgta gaatatcttg gtgaattaaa gtgacacgag
      961 tattcagttt taatttttct gacgataagt tgaatagatg actgtctaat tcaatagacg
     1021 ttacctgttt acttatttta gccagtttcg tcgttaaatg ccctttacct gttccaattt
     1081 cgtaaacggt atcggtttct tttaaattca attgttttat tatttggttg agtacttttt
     1141 cactcgttaa aaagttttga gaatatttta tatttttgtt catgtaatca ctccttctta
     1201 attacaaatt tttagcatct aatttaactt caattcctat tatacaaaat tttaagatac
     1261 tgcactatca acacactctt aagtttgctt ctaagtctta tttccataac ttcttttacg
     1321 tttccgccat tctttgctgt ttcgattttt atgatatggt gcaagtcagc acgaacacga
     1381 accgtcttat ctcccattat atcttttttt gcactgattg gtgtatcatt tcgtttttct
     1441 ttttgtgcgc ttcttgataa aagggatagt aattcattcc tggttgcaaa ttttgaaaac
     1501 cgctacggat cagatctagt cttataacta tactgacaat agaaacatta acaaatctaa
     1561 aacagtctta attctatctt gagaaagtat tggtaataat attattgtcg ataacgcgag
     1621 cataataaac ggctctgagc gcgagaccgt caattcgagg gggatcaatt ccgttttccg
     1681 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1741 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1801 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1861 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     1921 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     1981 gcagcccacc tatcacggaa ttgatccggc cacgatgcgt ggatggctga tgaaaccaag
     2041 ccaaccagga agggcagccc acctatcacg gaattgatcc ggccacgatg cgtccggcgt
     2101 agaggatctg aagatcagca gttcaacctg ttgatagtac gtactaagct ctcatgtttc
     2161 acgtactaag ctctcatgtt taacgtacta agctctcatg tttaacgaac taaaccctca
     2221 tggctaacgt actaagctct catggctaac gtactaagct ctcatgtttc acgtactaag
     2281 ctctcatgtt tgaacaataa aattaatata aatcagcaac ttaaatagcc tctaaggttt
     2341 taagttttat aagaaaaaaa agaatatata aggcttttaa agcttttaag gtttaacggt
     2401 tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat
     2461 tttgagtgac acaggaacac ttaacggctg acatgggggt ctcgctcctc acactggctc
     2521 accttcgggt gggcctttct gcgtttatac ctaggagtca aaattaggga ttaaatccac
     2581 tactagcgtt gagttagtta actttgcaat ttgttctagt gtttttatgg ttggatc
//
