LOCUS       sequence-404756-       15910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of Cascade and Cas3. Puromycin selectable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15910)
  AUTHORS   Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H,
            Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda 
            J, Mashimo T
  TITLE     CRISPR-Cas3 induces broad and unidirectional genome editing in human
            cells.
  JOURNAL   Nat Commun. 2019 Dec 6;10(1):5302. doi: 10.1038/s41467-019-13226-x.
  PUBMED    31811138
REFERENCE   2  (bases 1 to 15910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-6"; volume: "10(1):5302. doi"; pages: " 
            10.1038/s41467-019-13226-x"
COMMENT     Sequence Label: pPV-Dual_promoter-EF1-alpha-2xNLS-Cascade+Cas3-P 
            (RD)
FEATURES             Location/Qualifiers
     source          1..15910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   46..108
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    835..890
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(1143..1742)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1226..1246)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1723..1742
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(1743..1751,1752..1754,1755..2329)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1916..1935)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2145..2162
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    2371..2395
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2422..2442)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3958..3978)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3982..4035)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4045..4065)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4783..4803)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4807..4863)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4873..4893)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6028..6048)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6051..6060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6107..6131)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6154..7332)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6163..7101
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        7418..8596
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7649..8587
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    8619..8643
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8690..8699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8702..8722
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9248..9268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9278..9334
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9338..9358
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10001..10021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10031..10084
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10088..10108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12818..12838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(12865..12889)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    13119..13174
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   13877..13911
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(14004..14021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14175..14763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14255..14274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14934..15725,15726..15794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15557..15576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15795..15899)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctatta agtcgaggga tctccataag agaagaggga cagctatgac
      541 tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg aaaattttag
      601 ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa
      661 ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact gatgacctct
      721 ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca aactctaaac
      781 caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc cgagtgagag
      841 acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt agccagaagt
      901 cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctca
      961 gtggtatttg tgagccaggg cattggccac accagccacc accttctgat aggcagcctg
     1021 cacctgagga gtgaatttaa ctcgaggtat acttaagcct aggatccaga tctgtacatt
     1081 cgaatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     1141 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     1201 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     1261 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     1321 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     1381 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     1441 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     1501 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     1561 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     1621 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     1681 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     1741 atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg
     1801 ggggcctaga cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc
     1861 cagatcagat cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac
     1921 gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg
     1981 ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc
     2041 gccaggtggg gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga
     2101 agcttccaga ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag
     2161 aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac
     2221 attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta
     2281 ttccaagcgg cttcggccag taacgttagg ggggggggag ggagaggggg gggggcggaa
     2341 ttcgattcga ggccgcgttt tagctagatc accactttgt acaagaaagc tgggtctaga
     2401 tatctcgagt gcggccgctt acacctttct cttcttcttt gggctagctc cattagaagg
     2461 tcctccctgt ggcttcagct ccctcaggtg cttatacagg gtggcccttg ccagggccag
     2521 tgtgctgatc agcttggggt ggtgggcgta tggggccaca gactggttaa acagcatctc
     2581 gcacagctgg tgcagcttgt ccctcagatc ggcgatcact tcgtcggcct gggagaagtt
     2641 cacattggcc agcacatcgg ggatcagcag ctcggactgg cggtaaaagt gcctctcggc
     2701 ggtctcgtgc acgctcacgc ctgctccctt gaagtccttg ttcttaaagc cctcggcgaa
     2761 tgtatacagg gcctttctca gggcggtctt gtagcccagg cccactgtca cgatctcgtt
     2821 gatcacattg ccatactgct gccagccctg attaaacatc agcacatcgt gtctccgctc
     2881 caggatgctg gcctgattgt tcctgtagcc gcccatgatc agctccaggg gagactgtgg
     2941 ggcgatgttc ctgaactgat tcaccacggc ggccactctg ttgccattct cgttctggat
     3001 gatcttgtcc accaccactc tgctgatctg ggtccaagag ggggcggatg tggtaaaggc
     3061 caggaacttc tcctccacct cgcccttctt cactgtcacc agacatggag agtgagggtg
     3121 gggccacagg ccgttcactg taaaggtgaa cttctccttc aggaagccgg tgtacctcag
     3181 attgctttcc tggccacagc aagaacactt gccgatgccg atggggtcgc acagctcgat
     3241 gtgggctggc tgccaaaaca ggcctctcac gaagccgatg gagctggcag ggatgctctc
     3301 gttagacttg ataggcttga tccaggtggg ctggttctct gtgtggctct cattgggaaa
     3361 ctgcttctgc agccgtggca gggtcagcac attcagcagc actgtgctcc tcagatcgat
     3421 gcctctcacg aatgtggtca ctggggttcc tcccctcagt ccagacttaa agcctcctcc
     3481 gaatccaggt gcctggtttg cctgattaaa cagggcgatg gcggtacagc ctccgcacag
     3541 ggcctctccc tgtcctggct ggttcacgaa ggcgcaattt gttgctccgg acacgcctgc
     3601 cagcagcttc tccatggggg tcacgtcgtt ggccttcacg ccctttgtct gcatgaaggg
     3661 gtgctcggcg tgattcagat agaacatgtc gatccatggg gcgatcagct gctgaaactc
     3721 atcctcggtc agagggttca tgatgcggtg cctgaactcc acatcgtcct tggcaggggc
     3781 gatgatctgg ccgatgcaca ccagcagggc cagggcggcc agctccatat cgtctctggg
     3841 caggctcagt ctccactgat cccgggagca gtacaggctc tgcagattaa taatctggac
     3901 ttttcctccg ttccgtgggc gcacaggaat ccagttgtca atcagcaggt tggatcccac
     3961 ctttctcttc ttctttggca ttgggccggg attttcctcc acgtccccgc atgttagaag
     4021 acttcccctg ccctcgccgg agcccacctt tctcttcttc tttggtctag aggactcaaa
     4081 ttcactgcca tcagcagtcc gcttctggct cacgtccatt cctcccttga tcacatacca
     4141 ctccctggat gcaaactgcc ttggcagtgt gatcattggc tcatccctgg cggtgaactt
     4201 caggtggtgg cctgtcacgc tctcctcaga atagatgtcg ccgcccacag gctcgtagtt
     4261 cagcagggcc ttctggggat cggaggcctg acaggtgccc aggaacagtg ggtgtgtcag
     4321 agggcagctt ctccggccca gatagggggt gtaccgtggc ttcagcacgg ccttctccag
     4381 ctcgctgatg accattgttg cgtgtggggt cagccacagg gccactgtaa aagaggcgtc
     4441 gcacaggtac tcgcgccatg tctggatggt ctcgtgggac ttcaggcccc tatagtcctc
     4501 tctggcgccc agcacggtgt ggtaatcccg caggcctgtc acggacacgc gcctatcgtc
     4561 caggatcagc tcatcacagc gcacggcgaa ctgcacggac tcgctcaggg cctgcaggct
     4621 agaggtatcg tccctctgga tgcccaggca ggctcccagc agtcccagca gtccagaccg
     4681 ggtaggaaag cggcctgttg gcctggttcc ctcgaatgta ggctgtcccc atgcctgcat
     4741 tggtcctgcc agtctcagga tcaggtagga gcgttcacgc gtcacctttc tcttcttctt
     4801 tggcatgggg cctggattct cctccacatc gccggcctgc ttcagcaggc tgaagttggt
     4861 tgctccgctt cccacctttc tcttcttctt tggactagtg gactcaaatt cactgccatc
     4921 agcagtccgc tttgcctctc cattgttccg cacccagctc ttcagctgct ccagggttgg
     4981 catctgcttc acctgggctg tgatagggtc cacatcagac agggaaaact gggcggcagc
     5041 tccgttcagg ccatatccat tggccacgcg gtcccagtac tggttaaagg cctggatgga
     5101 aggctgcagg aagccatcct tggccttcac ggccttctcg aaggcattgg ccatagacag
     5161 tggcatgtcg gaaaagttca ccatgaccat atcggcagga ttgaaggcgg cgtaggtgcg
     5221 ctgctttgct cctggcacct cggttgccag catgtgcacc acgtgtgtgg cgatctccag
     5281 ggcctgctcc ctggatgctc ctcccaggtt ctcctgcagc tgtgccaggt tgatattggc
     5341 atatctgtaa aacacgccgg agctgaactc ctgggttccc aggtgggcgc ttccctgctc
     5401 ctgcagatcg tccacggctg tgaaccagtc gatatcagag tccacctggt gtgtggtgat
     5461 ggcgtgtgcg atggacattg ctccgtccac cttgcccagc tcggtcatca tgccggatgt
     5521 ggccatcctg ccgctcaggg cgatgtccac tccctgctgc aggttcactc tgatggcggc
     5581 gatatcctcc ttcagcacct tcagcagctt cttatcgtcc agattatcgg cctcggcctt
     5641 ggccacctgc tcacagaacc atgcgatctc tcccaccacc catggggtca ctgcgtcggc
     5701 gctgatcttc tcggcctcat ccacggactt gccgctcagc agggccagtg tcttgtcgat
     5761 gatcttctga tcaaaccgct cgcccagctt ctgtctcagc acgtccctca gctgtgccag
     5821 gtggatggtc ctcagagagg actcgccgat attctgggca tagtagccag actttctcat
     5881 ggcccgcttc agggactggc tagagatcct cactctccgc ttgccgccaa agatggcgtc
     5941 cttctgcatg ttcatatcgt ctctattcag gcagcttgga gagtgggaga tcagcacgtg
     6001 gatgttgatg aaattggatt cagatctcac ctttctcttc ttctttgggg ccatggtggc
     6061 ggcgaattcg gtaccggatc cagtcgactg aattggttcc ggcgccagcc tgcttttttg
     6121 tacaaacttg tgatggccgc caccgctaat tcctcacgac acctgaaatg gaagaaaaaa
     6181 actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa
     6241 ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt
     6301 gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa
     6361 gctcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc
     6421 gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc
     6481 cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc
     6541 cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg
     6601 cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct
     6661 cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc
     6721 gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac
     6781 aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag
     6841 agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc
     6901 ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg
     6961 cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag
     7021 ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg
     7081 ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt
     7141 cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca
     7201 gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt
     7261 gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg
     7321 ggcaccggag ccagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
     7381 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     7441 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     7501 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     7561 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     7621 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     7681 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     7741 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     7801 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     7861 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     7921 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     7981 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     8041 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     8101 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     8161 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     8221 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     8281 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     8341 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     8401 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     8461 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     8521 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     8581 atttcaggtg tcgtgaggaa ttagcggtgg cggccatcac aagtttgtac aaaaaagcag
     8641 gctggcgccg gaaccaattc agtcgactgg atccggtacc gaattcgccg ccaccatggc
     8701 cccaaagaag aagagaaagg tgagatctga agccgatgag atcgacgcaa tggcactgta
     8761 cagggcatgg cagcagctgg acaacggatc ttgcgcacag atcaggcgcg tgagcgagcc
     8821 tgatgagctg agggacatcc cagccttcta tcggctggtg cagccctttg gctgggagaa
     8881 tcctagacac cagcaggccc tgctgaggat ggtgttttgt ctgagcgccg gcaagaacgt
     8941 gatccggcac caggacaaga agagcgagca gaccacagga atctccctgg gacgcgccct
     9001 ggccaattcc ggccggatca acgagcggag aatcttccag ctgatcaggg ccgatcgcac
     9061 agccgacatg gtgcagctga ggcgcctgct gacccacgca gagcctgtgc tggattggcc
     9121 actgatggcc cgcatgctga catggtgggg caagcgggag agacagcagc tgctggagga
     9181 cttcgtgctg accacaaata agaacgccaa gcggactgct gatggcagtg aatttgagtc
     9241 cactagtcca aagaagaaga gaaaggtggg aagcggagca accaacttca gcctgctgaa
     9301 gcaggccggc gatgtggagg agaatccagg ccccatgcca aagaagaaga gaaaggtgac
     9361 gcgtgaatac ctgagcaaag tgatcatcgc aagggcatgg tccagggacc tgtatcagct
     9421 gcaccagggc ctgtggcacc tgttccctaa tagaccagat gccgccaggg acttcctgtt
     9481 tcacgtggag aagaggaaca cacccgaggg ctgtcacgtg ctgctgcagt ccgcccagat
     9541 gcccgtgagc accgcagtgg ccacagtgat caagaccaag caggtggagt tccagctgca
     9601 agtgggcgtg ccactgtact ttaggctgcg cgccaatccc atcaagacca tcctggataa
     9661 ccagaagcgc ctggactcta agggcaatat caagcggtgc agagtgcctc tgatcaagga
     9721 ggccgagcag atcgcctggc tgcagagaaa gctgggcaac gccgccaggg tggaggatgt
     9781 gcaccctatc agcgagcggc cacagtattt cagcggcgac ggcaagtccg gcaagatcca
     9841 gaccgtgtgc tttgagggcg tgctgaccat caacgatgcc ccagccctga tcgacctggt
     9901 gcagcaggga atcggacctg ctaagtcaat gggatgtggg ctgctgtcac tggcacctct
     9961 gaagcggact gctgatggca gtgaatttga gtcctctaga ccaaagaaga agagaaaggt
    10021 gggctccggc gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg
    10081 cccaatgcca aagaagaaga gaaaggtggg atccgaggag cccttcaagt acatctgcca
    10141 ctattggggc aagagctcca agagcctgac aaagggcaac gatatccacc tgctgatcta
    10201 ccactgcctg gacgtggcag cagtggcaga ttgttggtgg gaccagtccg tggtgctgca
    10261 gaataccttt tgtcggaacg agatgctgtc taagcagaga gtgaaggcct ggctgctgtt
    10321 ctttatcgcc ctgcacgata tcggcaagtt cgacatccgg tttcagtata agtccgccga
    10381 gtcttggctg aagctgaatc cagccacacc ctctctgaac ggccctagca cccagatgtg
    10441 cagaaagttc aatcacggcg ccgccggcct gtactggttt aaccaggact ctctgagcga
    10501 gcagagcctg ggcgatttct tttccttctt tgacgcagca cctcacccct acgagtcctg
    10561 gttcccttgg gtggaggcag tgaccggaca ccacggcttt atcctgcatt ctcaggacca
    10621 ggataagagc cggtgggaga tgccagccag cctggcctcc tacgccgcac aggataagca
    10681 ggccagagag gagtggatca gcgtgctgga ggccctgttc ctgaccccag caggactgtc
    10741 catcaatgat atcccacctg actgctctag cctgctggcc ggcttttgtt ccctggccga
    10801 ctggctgggc tcttggacca caaccaatac attcctgttt aacgaggatg caccaagcga
    10861 catcaacgcc ctgaggacct acttccagga tcggcagcag gacgcctcca gagtgctgga
    10921 gctgtccggc ctggtgtcta ataagaggtg ctacgaggga gtgcacgccc tgctggataa
    10981 cggctatcag ccaaggcagc tgcaggtgct ggtggacgcc ctgcctgtgg caccaggact
    11041 gacagtgatc gaggcaccta ccggcagcgg caagacagag accgccctgg cctatgcctg
    11101 gaagctgatc gatcagcaga tcgccgactc cgtgatcttc gccctgccaa cacaggccac
    11161 cgccaatgca atgctgaccc ggatggaggc ctccgcctct cacctgtttt cctctcccaa
    11221 cctgatcctg gcccacggca acagcaggtt caatcacctg tttcagtcta tcaagagccg
    11281 cgccatcaca gagcagggac aggaggaggc ctgggtgcag tgctgtcagt ggctgagcca
    11341 gtccaataag aaggtgttcc tgggccagat cggcgtgtgc accatcgatc aggtgctgat
    11401 ctctgtgctg cctgtgaagc acaggtttat caggggactg ggaatcggca gaagcgtgct
    11461 gatcgtggat gaggtgcacg cctacgacac atatatgaac ggcctgctgg aggccgtgct
    11521 gaaggcacag gcagacgtgg gcggctccgt gatcctgctg tctgccaccc tgccaatgaa
    11581 gcagaagcag aagctgctgg atacatacgg cctgcacacc gaccccgtgg agaacaattc
    11641 cgcctatcct ctgatcaatt ggaggggagt gaacggagca cagcggttcg acctgctggc
    11701 ccaccctgag cagctgccac caaggttttc tatccagccc gagcctatct gcctggccga
    11761 tatgctgcca gacctgacca tgctggagag gatgatcgct gccgccaatg caggagcaca
    11821 ggtgtgcctg atctgtaacc tggtggatgt ggcccaggtg tgctaccaga ggctgaagga
    11881 gctgaacaat acacaggtgg acatcgatct gttccacgcc cggtttaccc tgaatgaccg
    11941 gagagagaag gagaacagag tgatctctaa cttcggcaag aatggcaaga gaaacgtggg
    12001 cagaatcctg gtggccacac aggtggtgga gcagagcctg gacgtggatt tcgactggct
    12061 gatcacccag cactgccccg ccgatctgct gtttcagcgg ctgggcagac tgcacaggca
    12121 ccaccgcaag tatcggcctg ccggctttga gatcccagtg gccacaatcc tgctgcctga
    12181 cggagaggga tacggcaggc acgagcacat ctatagcaat gtgcgcgtga tgtggcggac
    12241 ccagcagcac atcgaggagc tgaacggcgc ctctctgttc tttccagatg cctaccgcca
    12301 gtggctggac agcatctatg acgatgccga gatggatgag cccgagtggg tgggcaatgg
    12361 catggacaag ttcgagtccg ccgagtgtga gaagcggttc aaggccagga aggtgctgca
    12421 gtgggccgag gagtactccc tgcaggataa cgacgagaca atcctggccg tgaccagaga
    12481 tggcgagatg tctctgcccc tgctgcctta tgtgcagaca agctccggca agcagctgct
    12541 ggatggccag gtgtacgagg acctgagcca cgagcagcag tatgaggccc tggccctgaa
    12601 cagggtgaat gtgcccttca cctggaagcg gagcttcagc gaagtggtgg atgaggacgg
    12661 cctgctgtgg ctggagggca agcagaatct ggatggctgg gtgtggcagg gcaactccat
    12721 cgtgatcaca tacaccggcg acgagggcat gaccagagtg atcccagcca accccaagaa
    12781 gagaaccgcc gacggcagcg agttcgagtc cgctagccca aagaagaaga gaaaggtgta
    12841 agcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg atctagctaa
    12901 aacgcggcct cgaatcgaat tcgattcgaa tgtacagatc tggatcctag gcttaagtat
    12961 acctcgagtt aaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
    13021 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
    13081 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
    13141 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
    13201 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
    13261 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
    13321 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
    13381 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
    13441 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
    13501 cctcgactta attaaccaac aagctcgtca tcgctttgca gaagagcaga gaggatatgc
    13561 tcatcgtcta aagaactacc cattttatta tatattagtc acgatatcta taacaagaaa
    13621 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat
    13681 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg
    13741 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa
    13801 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag
    13861 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatacgt
    13921 ataatacata tgattcagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    13981 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    14041 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    14101 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    14161 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    14221 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    14281 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    14341 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    14401 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    14461 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    14521 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    14581 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    14641 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    14701 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    14761 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    14821 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    14881 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    14941 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    15001 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    15061 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    15121 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    15181 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    15241 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    15301 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    15361 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    15421 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    15481 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    15541 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    15601 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    15661 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    15721 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    15781 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    15841 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    15901 acatttcccc
//
