LOCUS       sequence-404758-        1807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Elu ES2 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo15
FEATURES             Location/Qualifiers
     source          1..1807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..48
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(115..217)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      276..664
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      1214..1241
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1262..1807,1..114))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
ORIGIN
        1 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
       61 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      121 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      181 attatggtga aagttggaac ctcttacgtg ccgatcagtc aattcgaggg ggatcaattc
      241 cgtattgatc cggccacgat gcgtccggcg tagaggatct gaagatcagc agttcaacct
      301 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact
      361 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa
      421 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat
      481 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat
      541 aaggctttta aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca
      601 ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct
      661 gacatggggg tctcggagag cctgtcactg aacggcacca tcatcgtggc gcgcgacatt
      721 gcccacgcga agctgaaaga gctgctcgac aacggtgaag aactgccgca gtatgtaagg
      781 gtttttctta gcgggttgct ggacgcactg gaatgattga gctatgttaa aaaagtctta
      841 gataacagcg gttgtgaaag caacggcgag tctgtgtaag tgagcagggc tgtccatatg
      901 tcatgcgcat aattgtgcaa aagcataccg gcacagttcg aatttgtgcg gcggatcgag
      961 acatgtttaa aaatggcttg ccatattaaa catagtgtgt gataacacgt tttgggtcaa
     1021 acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga gtaaacacgt
     1081 tgtcgttgaa taccgcttct ctgccgaacc ttatattagt gcctcatgca gtaatgtctc
     1141 agttttatct cagtaatgcc tgcgggctaa gaaaacactc taaggaattt tgcaaattac
     1201 gagacctcac actggctcac cttcgggtgg gcctttctgc gtttatacct aggagtcaaa
     1261 attactcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctaccga
     1321 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1381 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1441 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1501 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1561 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1621 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1681 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     1741 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1801 tatccag
//
