LOCUS       sequence-404759-        4382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Combine with Gal4 to direct ACR1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, 
            Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, 
            Gonzalez-Bolivar S, Hurth C, Krieg M
  TITLE     Neural engineering with photons as synaptic transmitters.
  JOURNAL   Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.
  PUBMED    37024651
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9."
COMMENT     Sequence Label: pNMSB91
FEATURES             Location/Qualifiers
     source          1..4382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    244..338
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    377..471
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    510..604
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2335..2439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2440..2508,2509..3300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2658..2677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3471..4059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3960..3979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4213..4230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4347..4368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta
      121 actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat
      181 ggatacgcta acaacttgga aatgaaataa gcttgctagc ccatgattac gccaagctac
      241 gggcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
      301 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactccct agcccatgat
      361 tacgccaagc tacgggcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      421 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc
      481 cctagcccat gattacgcca agctacgggc ggagtactgt cctccgagcg gagtactgtc
      541 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      601 cgagcggaga ctccctaggg gcggccgcac tgactgggcc ggccaaaaat gcataaggtt
      661 ttgctggcac tgttctttat ctttctggca ccagcatccg cactggcagt ctccgaaccg
      721 gcctgcaggt cgattttttg caaattacga gcgttgtagg gggcggagcg ataggtccta
      781 taggttttgg tatatcatca ttcattcatt cattggtaca ttcatttacc caccttcctc
      841 tttctgagct tctctggagt tctgtgcttc ctttttccct tatctttata ctgtaatttt
      901 taactttcag gcattgattg gcgcgcctct agaggatcca tgagcagcat cacctgtgat
      961 cccgccatct acggcgaatg gagcagggag aaccagttct gcgtggagaa gagcctgatc
     1021 accctggacg gcatcaagta cgtccagctg gtgatggccg tcgtgagcgc ctgtcaggtg
     1081 ttcttcatgg tgaccagagc ccccaaggtg ccctgggaag ccatctacct gcccaccacc
     1141 gagatgatca cctattccct ggccttcacc ggaaacggct acatcagagt ggccaatggc
     1201 aagtacctgc cctgggccag aatggccagc tggctgtgca cctgccctat catgctgggc
     1261 ctggtgagca atatggccct cgtgaagtac aagagcatcc ccctcaaccc tatgatgatc
     1321 gccgcctcca gcatctgcac cgtgttcggc atcaccgcct ccgtggtgct agacccgctg
     1381 cacgtgtggc tgtactgttt catcagcagc atcttcttca tcttcgagat ggtggtggcc
     1441 ttcgccattt tcgccattac catccacgat ttccagacca tcggctcccc catgtccctg
     1501 aaggtggtgg agaggctgaa gctgatgagg atcgtgttct acgtgagctg gatggcctac
     1561 cctatcctgt ggagcttctc cagcaccggc gcctgcatca tgagcgagaa caccagcagc
     1621 gtgctgtacc tgctgggcga cgctctgtgc aagaacacct acggcatcct gctgtgggct
     1681 acaacctggg gcctgctgaa cggcaagtgg gacagggatt acgtgaaggg caggaacgtg
     1741 gacggcaccc tgatgcctga gtacgagcag gacctggaga agggcaacac cgagaggtac
     1801 gaggacgcca gagccggcga gacctaacca tacaagtaat ccggatgatc gacgccaacg
     1861 tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat
     1921 tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt
     1981 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
     2041 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
     2101 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
     2161 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa
     2221 ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccca
     2281 aaaggccttt tttttttggc gccgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2461 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2521 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2581 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2641 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2701 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2761 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2821 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2881 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2941 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3001 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3061 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3121 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3181 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3241 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3301 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3361 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4201 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4261 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4321 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4381 gc
//
