LOCUS       sequence-404760-        5414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Combine with Gal4 to direct TeNL expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, 
            Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, 
            Gonzalez-Bolivar S, Hurth C, Krieg M
  TITLE     Neural engineering with photons as synaptic transmitters.
  JOURNAL   Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.
  PUBMED    37024651
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9."
COMMENT     Sequence Label: pNMSB88
FEATURES             Location/Qualifiers
     source          1..5414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(34..39,40..57,58..64)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    72..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     77..99
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     96..112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     96..112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    277..371
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    410..504
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    543..637
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(1384..1403)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2470..2547
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     promoter        3400..3504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3505..3573,3574..4365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3723..3742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4536..5124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5025..5044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5278..5295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(5412..5414,1..19)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
       61 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      121 cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt aaattttcag
      181 agcttaaaaa tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa
      241 taagcttgct agcccatgat tacgccaagc tacgggcgga gtactgtcct ccgagcggag
      301 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      361 tgtcctccga gcggagactc cctagcccat gattacgcca agctacgggc ggagtactgt
      421 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
      481 ccgagcggag tactgtcctc cgagcggaga ctccctagcc catgattacg ccaagctacg
      541 ggcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      601 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactcccta ggggcggccg
      661 cactgactgg gccggccaaa aatgcataag gttttgctgg cactgttctt tatctttctg
      721 gcaccagcat ccgcactggc agtctccgaa ccggcctgca ggtcgatttt ttgcaaatta
      781 cgagcgttgt agggggcgga gcgataggtc ctataggttt tggtatatca tcattcattc
      841 attcattggt acattcattt acccaccttc ctctttctga gcttctctgg agttctgtgc
      901 ttcctttttc ccttatcttt atactgtaat ttttaacttt caggcattga ttggcgcgcc
      961 tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     1021 taacatagaa cattttcagg aggacccttg gctagcgtcg acggtaccgg tagaaaaatg
     1081 gtgagcaaag gagaagaatt attcacggga gttgtaccaa tcctcgtcga gctggacgga
     1141 gacgtcaacg gacacaagtt cagcgtgtcc ggagagggag agggagacgc cacctacgga
     1201 aagctcaccc tcaagttcat ctgcaccacc ggaaagctgc cagtcccatg gccaaccctc
     1261 gtcaccaccc tctcctgggg agtccagtgc ttcgcccgtt acccagacca catgaagcaa
     1321 cacgacttct tcaagtccgc catgccagag ggatacgtcc aagagcgcac catcttcttc
     1381 aaggacgacg gcaactacaa gacccgtgcc gaggtcaagt tcgagggaga caccctcgtc
     1441 aaccgcatcg agctcaaggg aatcgacttc aaggaggacg gcaacatcct cggacacaag
     1501 ctcgagtaca actacttctc cgacaacgtc tacatcaccg ccgacaagca aaagaacgga
     1561 atcaaggcca acttcaagat ccgccacaac atcgaggacg gaggagtcca actcgccgac
     1621 cactaccaac aaaacacccc aatcggagac ggcccagtcc tcctcccaga caaccactac
     1681 ctgtccaccc aatccaagct gtccaaggac ccaaacgaga agcgtgacca catggtcctg
     1741 ctcgagttcg tcaccgccgc cgggggattc gaggacttcg tcggagactg gcgtcaaaca
     1801 gccggataca acctcgacca agtcctcgag caaggaggag tctcctcctt gttccaaaac
     1861 ctcggagtct ccgtcacccc aatccaaagg atcgtcctct ccggagagaa tggactcaag
     1921 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gatcgacatc
     1981 catgtcatca tcccatacga gggaccatgg atgcacgacc aactcaccga ggagcagatc
     2041 gccgagttca aggaggcctt ctccctcttc gacaaggacg gagacggcac catcaccacc
     2101 cgtgagctcg gaaccgtcat gcgttccctt ggacaaaacc caaccgaggc cgagctccaa
     2161 gacatgatca atgaagtcga cgccgacgga aacggaacca tctacttccc tgagttcctc
     2221 accatgatgg cccgtaagat gaaggacacc gacagcgagg aggagatccg tgaggccttc
     2281 cgtgtcttcg acaaggacgg gaacggatac atctccgccg cccaactccg tcacgtcatg
     2341 accaacctcg gagtgaagct caccgacgag gaggtcgacg aaatgatacg tgaggccgac
     2401 atcgacggag acggacaagt aaactacgag gagttcgagc aaatgatgac cgccaaggga
     2461 ggaggatcca agaggcgttg gaagaaaaac ttcatcgccg tctccgccgc caaccgtttc
     2521 aagaagatct cctcctccgg agcactcgag ctcctctccg gagaccaaat gggacaaatc
     2581 gagaagattt tcaaggtcgt ctacccagtc gacgaccacc actttaaggt catcctccac
     2641 tacggaaccc tcgtcatcga cggagttacc ccaaacatga tcgactactt cggacgtcca
     2701 tacgagggaa tcgccgtgtt cgacggaaag aagatcaccg tcaccggaac cctctggaac
     2761 ggaaacaaaa tcatcgacga gcgtctcatc aacccagacg gatccctcct cttccgtgta
     2821 accatcaacg gagtcaccgg atggcgtctc tgcgagcgta tcctcgcgta aggtaccgaa
     2881 ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa
     2941 attttaaata ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt
     3001 tttcttgctt tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg
     3061 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc
     3121 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga
     3181 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg
     3241 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag
     3301 caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gcccaaaagg cctttttttt
     3361 ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5341 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5401 cgcaacgcaa ttaa
//
