LOCUS       sequence-404762-       14561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 
            3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, 
            Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, 
            Gonzalez-Bolivar S, Hurth C, Krieg M
  TITLE     Neural engineering with photons as synaptic transmitters.
  JOURNAL   Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.
  PUBMED    37024651
REFERENCE   2  (bases 1 to 14561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9."
COMMENT     Sequence Label: pNMSB99
FEATURES             Location/Qualifiers
     source          1..14561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1564..1575)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3580..3596
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3580..3596
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3597..3643
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3757..3779
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3771..3788
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3772..3788
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3870..3890
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6419..6452
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     terminator      7262..7585
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7595..7628
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(10677..10693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10677..10693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12619..12641
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12679..12697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12765..12869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12870..12938,12939..13730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13088..13107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13901..14489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14390..14409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccagcgga
       61 taacaatttc acagggccct gtcggattat gggctcttct tttatcctct actttctttc
      121 tagaaaatgc aaatgttgag tcagaaattg ctgaatggag tacaactgca atgggtttag
      181 cttatgtttc tggaacaatt actggtggaa ttgtgattga aaagtaaggt tatatttcat
      241 ttcaataaat gttaccacag tataacaaaa acaagttaca ttgttcagag tatgtctgaa
      301 tgtattatta ttattgtatt tgtgcatcag tactgtagtt gtactgaaga agggtttaaa
      361 ataatctcaa cgtttttaag acgagttagt gagaatacgg tcgcagttag gatgtttgga
      421 catagtttta ttagataatc gtgaactaac tcactataaa aacccgctaa ctcattgaat
      481 aagatagttt taaacgttca aaatttgaag acaaaattgt atgaataagt ttacagagtt
      541 ggtcgacgca agcttctcct tctcttcaca tttctcaata atttagctct aattgcattc
      601 gtattcttcg caaaaatacg tattctgatt gacccgatga aatacggatg cctagttgca
      661 ttgatcatct acggttatac ttatgggtga ggtttttcaa atatattttg atttaatcga
      721 ataatgaaat ttcagaactg gagttggtcc aatttcgtgg tttatatcct ctgaactggt
      781 acctcaaaaa catcgtagta ttgctcaatc tgtggcatat tcaatgaata ctgtaatggt
      841 ggttatcagt acatttacag tattacctct atattccgtt attggtagtt atgctttcct
      901 tattctatat tcaattcctt catttataag tatgcttata ttatttcgct acttaccgga
      961 aaccaaagga cgagagattc atgatattgt gaatgaactt aaaaaaaaat gaaaaccttt
     1021 gaaaacgttc atacggccga aattttactt gatcgagcca attgaacttt tgttaagact
     1081 tacagaaaag tgagagattt tgttacaaaa aagtgagaaa aaagcgaaaa tgaacaggaa
     1141 aaagaagaaa aatacacccg aagtatcata taaaaattaa aaagattgtt tgacctggcg
     1201 gaactttttt gcaaaaactc tgaatatcca tggcacatgc ttcgtgcaaa acaggatcca
     1261 aatgaatatc aacaagagat tcttgaagac gacgagccac ttgctcagag cattgcttat
     1321 ctcgaatgag acccttgtta aaatcatttt tcaaacatta tatgacattg tcgaaatgtc
     1381 ctcctgattc catgatggta gcaaactcac ttcgtctttg agccaattta tccaagtcct
     1441 tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat caaaaacaat
     1501 ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg gctcaaagtt
     1561 agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc ccaacaaggg
     1621 atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca aattcatcgc
     1681 ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt ctgcacccta
     1741 tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt ctccccagct
     1801 ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct ctctgacgtc
     1861 ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt ttcccatttc
     1921 tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt ttttttctgt
     1981 cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc tttttcttct
     2041 tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac cccgcggttc
     2101 gcaataggtt tctttttcaa attattagcg ttataaatag aaaaatggtg gagtttcaat
     2161 aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt tgaaattttt
     2221 cataccagtt ttccggaaat ccatctgata tcattatcgt atttttcttt ttaaaaatgt
     2281 tttcaaaaaa aaacaaaata tagctggtta tttggcaccc tctaattacc attttcctgt
     2341 cacaccacac tcttttcctt cttccacttc tttccgtttt cagccgcttc caaccaaacc
     2401 gatatgaaag ccgagcaaca acgatcgatt ccacccggct cggcgacctt cccgtcgcag
     2461 gtgagactca gaaaactaga gaaaccgctc aactaactct tgatatccga tttcattctt
     2521 ttctcttttc tttttgttga acttttccat atttccagat gccacggcca ccaccaagca
     2581 ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa agctcaaatc actccgaacg
     2641 acgttttagc acttccggga atcactcaag gtatcttgct ccctttggat ttcagtttaa
     2701 aacataaatt taggattctt atgctcccca tctgcgaaca tctataacat cgagttcacc
     2761 aagttccaaa tccgtgatct ggacactgag caagtgcttt tcgagatcgc caaaccggag
     2821 aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg ttatagattt
     2881 gctccaaact ttttgaaact caaaacggtc ggagcaactg tggaattcaa agtaggagac
     2941 atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg ccttctgaag
     3001 tgttttgact ttgaattcgg attctgtatt ccgaattcac gaaacaactg tgaacatatc
     3061 tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa agtacaattc
     3121 aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga gactcgttct
     3181 gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga ctactcatat
     3241 gatgcataaa tatttaatac aaaaatgttc tggataatta ttctgtcgaa tagaaaaaaa
     3301 actccaaatg tgattaaatt ccaataattc ctgtctagtt tgttccttcc ccttcccctt
     3361 ctcatgttca atgcattcct aagcttttca gttcccccct tgtttctata ttttttcgct
     3421 gtcctgtcac actcgctaaa aacactaatc acacggaaat ctgttttcaa taaaaactcc
     3481 aactttaact cattttcaat ttcaactgaa agattttttc attagagaat gtctagaact
     3541 aggcccgggc tacgtaatac gactcactta aggtgccgct ctagaactag tggatccaaa
     3601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcgcatt
     3661 atacgagacg tccaggttgg gatacctgaa acaaaaccca tcgtacggcc aaggaagtct
     3721 ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt tgtaaaacga
     3781 cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc cgcctgacct
     3841 cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg agtgattata
     3901 gtctctgttt tcgttaattt tgaattttgc ttgataaggc tgcaacaaag atcaggttga
     3961 catatatttt cagtaattta ttttaacctg tactctatca ctgccggcta taataagttc
     4021 ttgaataaaa taattttccc gacaaaacat gagtatttct ttcgaaaata aaagtgcagg
     4081 ctaattagag attattctgt aattaactgc ataatttgtc acgtgccata gttttacatt
     4141 ccactacgtc atagttctta aaatactaat ctcctgaaaa tagaagtagg tgaagaaagt
     4201 ttaattatca gttctaaaat gacaattgat ctttggaata tgttctgaaa ctaccgatca
     4261 ttgaacagat gctatttgaa tgatatagaa ttgtatattt gcaatttctg aaacgcgttc
     4321 ttaaaggcac acagattaat tcaaaagggt ctggccgcaa aaaggtttat ggtggccgat
     4381 tttgagtttt gtgtgtgatt gctttttcac aatcagtgtt ttcaggatta tgtgatgaac
     4441 tagatcttca agtttcgtta catttcatat gttttcggaa ctcacgaagt acatattggg
     4501 tattgtgctc aaaaaattca gcaatcagct tcgctccgct gactttagaa cccaaaaaaa
     4561 tagtatggcc aaactgactg tgttacgatc atttcaattt ttcaatacat atttaagatt
     4621 tctaagagta agaaggtcaa aaactgttct ggaatacata tatatttttc aggttacaat
     4681 tagtcaaaaa gtgcactgaa atatacgttt taatttcacg aataacccaa ttagttcaat
     4741 gtatttttgg tcaaccaacg ttaaagtttg gcttccaacc aattatcatt tctgatcaac
     4801 cacaatgttt tttctttatc tgcaagttaa ttttttattt ttatccagat gtttggcata
     4861 tttttcaatt cttcactagc gcccacttct tgcacttccg gcgccctgaa tctaatgcat
     4921 ctgttgcaag aattgaaaga ccaatcaaca cattgttttc ttcacgagat actgaagaaa
     4981 atgaataaaa acagagaaaa agagccatgt gattagtgac aactgttgct aacagatacc
     5041 atagcttgga cttggtacgt gatggcaacg tatgggtcaa caaaaatgat tgcagagggg
     5101 gtgcaaaaca gtcaagtcga gaaaatatga aaaacagaaa acaaagaaca gaaaaatggg
     5161 tttgagagtc agtataattt ataaaagaaa aattgtacat agaaattaac catttttgta
     5221 gaagaagtta tttttcaagc atcgttaaaa attattcaaa gcaccttatt tcatatttaa
     5281 ttttaaacat ggttaaatga acaacacggt gcgcaatcag gaaaacttga aatctgaaac
     5341 tgttgttgtg atcttcttcg caactgttca gatagcacta gtgtaatgtt aagagtgcgc
     5401 gaatataatg gaatataatg gatcacacct cctgccatca ggtaaacgtc tctgttatca
     5461 catatttcca actattaaat ttttaccttt tacagtttta catttttttg aaaaaagtaa
     5521 ctttttgtct tcaaaatccc tgacgaaaat atcaaatatt ttaatcgaga ctgcagagga
     5581 accgattgat gatttggaaa atccagcttt acctgtgtaa gaactgaaaa gtttcataac
     5641 cctagggtat tcccagttac attccccact ggctaacaat agcacccagt ttttcatcac
     5701 ctttcttcaa atttctcggc gatttgttaa aaacaaaatt tgtgtccctt ctctgatatc
     5761 tctatgtctc taaacacaag ttcatcggaa aacgaaggag ggtaggtgtt ggttgggctc
     5821 ccgaagtgaa aatagaagag caagaataga atattagaga gagagtgcag agagggcggg
     5881 atagctcccg ggattccgtt ttcttcttct ttatcttcaa cgatgatgtg tgtgcgtgtt
     5941 gtatagattc tgttgctccc ccacaactcg ctccgaaggc tcaatacaat tcaattgata
     6001 ttggaggaga gcctaccgga gtgggaggat aagaagaaac ataagaagaa gaagaagaag
     6061 aagcatgctt ctggtttttg atgctatgaa aacggcacaa aaagatgatt gaggtccctt
     6121 ttcaatacct tctctcatct ttcaaatccc attgaaacct aaaacttctc accacgcttt
     6181 accattgttc tccaaaaact tatagcaatg tctataactt ttttatctct gaaaagcagt
     6241 gttccatttt tctttttcct attttatttc aattgtttct cacatttcgt ttggattctt
     6301 tgcttgtcaa ccagcttctt cttccacttt taccgtctaa ttttcagggc agggagccat
     6361 caaacccacg accactagat ccatctagaa tccccgggat tggccaaagg acccaaaaat
     6421 aacttcgtat aaagtatcct atacgaagtt atatgtccga actcatcaag gagaacatgc
     6481 acatgaagct ctacatggag ggaaccgtag acaaccacca cttcaagtgc acctccgagg
     6541 gagagggaaa gccatacgag ggaacccaaa ccatgcgtat caaggtcgtc gagggaggac
     6601 cactcccatt cgccttcgac atcctcgcca cctccttcct ctacgggtcc aaggtaagtt
     6661 taaacatata tatactaact aaccctgatt atttaaattt tcagaccttc atcaaccaca
     6721 cccaaggaat cccagacttc ttcaagcaat ccttcccaga gggattcacc tgggagcgtg
     6781 tcaccaccta cgaggacgga ggagtcctca ccgccaccca agacacctcc ctccaagacg
     6841 gatgcctcat ctacaacgtc aagatccgtg gagtcaactt cacctccaac ggaccagtca
     6901 tgcaaaagaa gaccctcgga tgggagacct tcaccgagac cctctaccca gccgacggag
     6961 gactagaggg acgtaacgac atggccctca agctcgtcgg aggctcccac ctcatcgcca
     7021 acgccaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcaga
     7081 ccacctaccg ttccaagaag ccagccaaga acctcaagat gccaggagtc tactacgttg
     7141 actaccgtct agagcgtatc aaggaggcca acaacgagac ctacgtcgag caacacgagg
     7201 tcgccgtcgc ccgttactgc gacctcccat ccaagctcgg acacaagctc aactaagata
     7261 aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct ttttgtcaaa
     7321 aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact ttttatagtc
     7381 actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta
     7441 ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag gttgagaaaa
     7501 atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa agaggaaact
     7561 ttattgtgcc gccaagaaaa aagtctcagc cggcataact tcgtataaag tatcctatac
     7621 gaagttatat ggattatgga ggcgccctga gtgccgttgg gcgcgagctg ctatttgtaa
     7681 cgaacccagt agtcgtcaat ggctctgtac ttgtgcctga ggaccagtgt tactgcgcgg
     7741 gctggattga gtcgcgtggc acaaacggtg cccaaacggc gtcgaacgtg ctgcaatggc
     7801 ttgctgctgg cttctccatc ctactgctta tgttttacgc ctaccaaaca tggaagtcaa
     7861 cctgcggctg ggaggagatc tatgtgtgcg ctatcgagat ggtcaaggtg attctcgagt
     7921 tcttcttcga gtttaagaac ccgtccatgc tgtatctagc cacaggccac cgcgtccagt
     7981 ggttgcgtta cgccgagtgg cttctcacct gcccggtcat tctcattcgc ctgtcaaacc
     8041 tgacgggctt gtccaacgac tacagcaggc gcaccatggg tctgcttgtg tcttgcatcg
     8101 gaaccattgt gtggggcgcc acttccgcca tggccaccgg atacgtcaag gtcatcttct
     8161 tctgcctggg tctgtgttat ggtgctaaca cgttctttca cgctgccaag gcctacatcg
     8221 agggttacca caccgtgccg aagggccggt gtcgccaggt ggtgactggc atggcttggc
     8281 tcttcttcgt atcatggggt atgttcccca tcctgttcat cctcggtccc gagggtttcg
     8341 gtgtcctgag cgtgtacggt tccaccgtcg gccacaccat cattgacctg atgtcgaaga
     8401 actgctgggg tctgctcggc cactacctgc gcgtgctgat ccacgagcat atcctcatcc
     8461 acggcgacat tcgcaagacc accaaattga acattggtgg cactgagatt gaggtcgaga
     8521 cgctggtgga ggacgaagcc gaagctggtg cagtacctgc cgctgccacc taagctgtct
     8581 catcctactt tcacctagtt aactgcttgt cttaaaatct atgcttctct ttagtatcta
     8641 aaattttcct agaagcttac aagtatataa atggtctctt ctcaataaag gttgtatatt
     8701 tattcatctt attgaatctg ccatttcctc gtttttgcga gtttatatac cttccaattt
     8761 tctttctatt gtattttcaa cttctaattt taattcaggg aaactgctgt accggtagaa
     8821 aaaatggtcg actcatcacg tagaaaatgg aacaaagctg gtcacgctgt ccgtgccatc
     8881 ggacgtctct cctctccagt cgtctccgag cgtatgtacc cagaggacgg agccctcaag
     8941 tccgagatca agaagggact ccgtctcaag gacggaggac actacgccgc cgaggtcaag
     9001 accacctaca aggccaagaa gccagtccaa ctcccaggag cctacatcgt cgacatcaag
     9061 ctcgacatcg tctcccacaa cgaggactac accatcgtcg agcaatgcga gcgtgccgag
     9121 ggacgtcact ccaccggagg aatggacgag ctctacaagg gaggaaccgg aggttccctc
     9181 gtctccaagg gagaggagga caacatggcc atcatcaagg agttcatgcg tttcaaggtc
     9241 cacatggagg gttccgtcaa cggacacgag ttcgagatcg agggagaggg agagggacgt
     9301 ccatacgagg ccttccaaac cgccaagctc aaggtcacca agggaggacc actcccattc
     9361 gcctgggaca tcctctcccc acaattcatg tacggctcca aggcctacat caagcaccca
     9421 gccgacatcc cagactactt caagctctcc ttcccagagg gattccgttg ggagcgtgtc
     9481 atgaacttcg aggacggagg aatcatccac gtcaaccaag actcctccct ccaagacgga
     9541 gtcttcatct acaaggtcaa gctccgtgga accaacttcc caccagacgg accagtcatg
     9601 caaaagaaga ccatgggatg ggaggccacc cgtgacgacc tcaccgagga gcaaatcgcc
     9661 gagttcaagg aggccttctc cctcttcgac aaggacggag acggaaccat caccaccaag
     9721 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca ggagctcgga
     9781 accgtcttcc gttccctcgg acaaaaccca accgaggccg agctccaaga catgatcaac
     9841 gaggtcgacg ccgacggaga cggaaccttc gacttcccag agttcctcac catgatggcc
     9901 cgtaagatga acgacaccga ctccgaggag gagatccgtg aggccttccg tgtcttcgac
     9961 aaggacggaa acggatacat cggagccgcc gagctccgtc acgtcatgac cgacctcgga
    10021 gagaagctca ccgacgagga ggtcgacgag atgatccgtg tcgccgacat cgacggagac
    10081 ggacaagtca actacgagga gttcgtccaa atgatgaccg ccaagtaagg taccgaattc
    10141 gctagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt
    10201 ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt
    10261 cttgctttct aaaaaattaa ttcaatccaa atctaaacat ttttttttct ctttccgtct
    10321 cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg
    10381 ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga
    10441 tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat atggaggaag
    10501 gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa
    10561 agaaattagg cgaacgatga gaattgtcct cgcttgggcc ctctgacttc tcccgggcaa
    10621 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
    10681 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
    10741 ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa
    10801 agaacatgtg agcaaaaggc cagcaaaagg ccaggactag tgagtcgtat tataagtgca
    10861 agtaagatca gtgtttgttt cgatatcagt ctgtttcgta acggtcttct gtataactac
    10921 aaaaaagaat aaaaaaccgt atcacaactg agaaaaaaga caaaacttat tgaacagtgt
    10981 tcctttcaaa aaattatttt gtaaaaaatc gagtttttct ccaaaataac gcacgcaatc
    11041 aaaaacaaga aaactaacgt gttctcctgg gcgtggaact ccttatcaag attaccatta
    11101 ttgtctgtca gaattgatat ttcaattatt tcatatctag acaaacccgg gtgtcttgca
    11161 tttattagta ctgtgagttt acatacaaac gaggcttact cagataacat agagtcacct
    11221 ctaattagca aaaacccatt tctaattagc tatttcgacc agttctaaag tcaccgtaat
    11281 tagaaatggt ttttgtagct tttctaatta gaaatgaccc cgagataagt gaaatatggc
    11341 attttctggg ttttctagta agaaatgatc ccgcacgtag tgattccgcg ttacaagcca
    11401 aattcaaatt aaaaaagtat attacacaat tcaagttaca ttattttcag gaaacaatgg
    11461 cgaagagcaa agcagagatt caaaaggagg aaagagacaa cagatatgaa cgaagacttc
    11521 ttgaaaagag aaagagacat gaggtgagta accatgataa taaataatca agtaagggct
    11581 atagatttat aaattctgga gttaacagtt tcgaaaaaaa gtagttttca tatgtgcgaa
    11641 ggaatatcaa aaatagggga atagcctaaa cgagtgagaa aattaaatgt ttgttatttt
    11701 ttcaaaaatg tctgcagggc cgttatcctt tcaaatgcac tctatcaaac atagatcata
    11761 tccatcatat cttatccaga atttatatca tatccaagat tgagagcgtt tcaaggaaaa
    11821 tctcaaacaa ccatacaatt ttgtagatcg acaacaaggc ctccacttct tcatcgatgg
    11881 cctctgcagc acctgcacca ccaagtttgt atttttcata ttaatataaa gtgagccacg
    11941 catagtcgtt tttaggattt ttgccagatc gatgtcgcgc aaacagactg tttttttttc
    12001 tttgaaagcc gtgcgtgaca tgggccacgg attgtaacgc cgcgctggat ggataaaaat
    12061 ttattcagtt ttttagcctt tacaaaatat ggttcgacta ctttctttat ttttacattt
    12121 ttataggtgc tcattgagct gagatatttt gggtataatc ggaaaatcgc tgattttggt
    12181 catttttctg cactaaagat tacacaaaaa gttcagtatt tagattcatt gttgaaaatt
    12241 ggggatgatc tacagataca tatcgccgtg ttcaaaaaca cacgtgctgt gacgtcgtga
    12301 ctggggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    12361 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    12421 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
    12481 gcatatggtg cactctcagt gcaatctgct ctgatgccgc atagttaagc cagccccgac
    12541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    12601 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    12661 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
    12721 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    12781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    12841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    12901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    12961 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    13021 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    13081 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    13141 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    13201 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    13261 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    13321 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    13381 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    13441 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    13501 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    13561 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggtcagatg
    13621 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    13681 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    13741 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    13801 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    13861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    13921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    13981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    14041 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    14101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    14161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    14221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    14281 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    14341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    14401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    14461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    14521 tggtcttttg ctggcctttt gctcacatgt tctttcctgc g
//
