LOCUS       sequence-404765-       13786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 
            3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, 
            Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, 
            Gonzalez-Bolivar S, Hurth C, Krieg M
  TITLE     Neural engineering with photons as synaptic transmitters.
  JOURNAL   Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.
  PUBMED    37024651
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9."
COMMENT     Sequence Label: pNMSB96
FEATURES             Location/Qualifiers
     source          1..13786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1568..1579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3584..3600
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3584..3600
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3601..3647
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3761..3783
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3775..3792
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3776..3792
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4583..4594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5705..5738
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     terminator      6548..6871
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6881..6914
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(9906..9922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9906..9922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11848..11870
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11908..11926)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11994..12098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12099..12167,12168..12959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12317..12336)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13130..13718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13619..13638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag
       61 cggataacaa tttcacaggg ccctgtcgga ttatgggctc ttcttttatc ctctactttc
      121 tttctagaaa atgcaaatgt tgagtcagaa attgctgaat ggagtacaac tgcaatgggt
      181 ttagcttatg tttctggaac aattactggt ggaattgtga ttgaaaagta aggttatatt
      241 tcatttcaat aaatgttacc acagtataac aaaaacaagt tacattgttc agagtatgtc
      301 tgaatgtatt attattattg tatttgtgca tcagtactgt agttgtactg aagaagggtt
      361 taaaataatc tcaacgtttt taagacgagt tagtgagaat acggtcgcag ttaggatgtt
      421 tggacatagt tttattagat aatcgtgaac taactcacta taaaaacccg ctaactcatt
      481 gaataagata gttttaaacg ttcaaaattt gaagacaaaa ttgtatgaat aagtttacag
      541 agttggtcga cgcaagcttc tccttctctt cacatttctc aataatttag ctctaattgc
      601 attcgtattc ttcgcaaaaa tacgtattct gattgacccg atgaaatacg gatgcctagt
      661 tgcattgatc atctacggtt atacttatgg gtgaggtttt tcaaatatat tttgatttaa
      721 tcgaataatg aaatttcaga actggagttg gtccaatttc gtggtttata tcctctgaac
      781 tggtacctca aaaacatcgt agtattgctc aatctgtggc atattcaatg aatactgtaa
      841 tggtggttat cagtacattt acagtattac ctctatattc cgttattggt agttatgctt
      901 tccttattct atattcaatt ccttcattta taagtatgct tatattattt cgctacttac
      961 cggaaaccaa aggacgagag attcatgata ttgtgaatga acttaaaaaa aaatgaaaac
     1021 ctttgaaaac gttcatacgg ccgaaatttt acttgatcga gccaattgaa cttttgttaa
     1081 gacttacaga aaagtgagag attttgttac aaaaaagtga gaaaaaagcg aaaatgaaca
     1141 ggaaaaagaa gaaaaataca cccgaagtat catataaaaa ttaaaaagat tgtttgacct
     1201 ggcggaactt ttttgcaaaa actctgaata tccatggcac atgcttcgtg caaaacagga
     1261 tccaaatgaa tatcaacaag agattcttga agacgacgag ccacttgctc agagcattgc
     1321 ttatctcgaa tgagaccctt gttaaaatca tttttcaaac attatatgac attgtcgaaa
     1381 tgtcctcctg attccatgat ggtagcaaac tcacttcgtc tttgagccaa tttatccaag
     1441 tccttgtaaa aagtattcga aaattgttaa cggataaaat gtttattata atatcaaaaa
     1501 caatttgtca gttgaccact ttttgatata tttgacagaa accgggatga attggctcaa
     1561 agttagggcg cccttctatt acagggtttc tgataacaaa ccggttatta actcccaaca
     1621 agggatgatt tcaattcatc atgctcaaat tgacccaaat taagttacat gacaaattca
     1681 tcgccctttt caactcttct tggctcatca tctgttattc tgttcctatt ctgtctgcac
     1741 cctatacccc tttgcatact ctctcgtcat tcctttcttg gatagtgtct tcttctcccc
     1801 agctctgcta cttctatgac ttgcgcccgc tgcttttccg ctcgttcctc tcctctctga
     1861 cgtcttcttc tgcttctttt ctccttcctt tttcccttct caatcttcct ctttttccca
     1921 tttctgtcaa tcattcgaag aagaagaaga agaagaccct cattcatttc attttttttt
     1981 ctgtcggtgt gtgctgctcg gttaagagtg agctctctat tccacgtctt cttctttttc
     2041 ttcttcgatt cgaatcaatc actccaccaa aacgcattcg tttttgggat tcaccccgcg
     2101 gttcgcaata ggtttctttt tcaaattatt agcgttataa atagaaaaat ggtggagttt
     2161 caataaaaat gataatttac aaagtgtatt ttgattacat gtactcaaaa aggttgaaat
     2221 ttttcatacc agttttccgg aaatccatct gatatcatta tcgtattttt ctttttaaaa
     2281 atgttttcaa aaaaaaacaa aatatagctg gttatttggc accctctaat taccattttc
     2341 ctgtcacacc acactctttt ccttcttcca cttctttccg ttttcagccg cttccaacca
     2401 aaccgatatg aaagccgagc aacaacgatc gattccaccc ggctcggcga ccttcccgtc
     2461 gcaggtgaga ctcagaaaac tagagaaacc gctcaactaa ctcttgatat ccgatttcat
     2521 tcttttctct tttctttttg ttgaactttt ccatatttcc agatgccacg gccaccacca
     2581 agcaccgaac aaggaatcac aacggaatcg gagcttgcga agaaagctca aatcactccg
     2641 aacgacgttt tagcacttcc gggaatcact caaggtatct tgctcccttt ggatttcagt
     2701 ttaaaacata aatttaggat tcttatgctc cccatctgcg aacatctata acatcgagtt
     2761 caccaagttc caaatccgtg atctggacac tgagcaagtg cttttcgaga tcgccaaacc
     2821 ggagaacgat caggagaatg atgagtcgcc acaggagtcg gcaagatacg tgcgttatag
     2881 atttgctcca aactttttga aactcaaaac ggtcggagca actgtggaat tcaaagtagg
     2941 agacatccca atccatcatt tccgaatgat cgaacgtcac ttcttcaaag atcgccttct
     3001 gaagtgtttt gactttgaat tcggattctg tattccgaat tcacgaaaca actgtgaaca
     3061 tatctatgag ttccctcaac tctctcaaca actcagtgag tcattattct aaaaagtaca
     3121 attcaaaaga ctaatctctt tttcagtgga cgacatgatc aacaatccaa acgagactcg
     3181 ttctgacagc ttctatttcg tcgataacaa actcgtcatg cacaacaaag ccgactactc
     3241 atatgatgca taaatattta atacaaaaat gttctggata attattctgt cgaatagaaa
     3301 aaaaactcca aatgtgatta aattccaata attcctgtct agtttgttcc ttccccttcc
     3361 ccttctcatg ttcaatgcat tcctaagctt ttcagttccc cccttgtttc tatatttttt
     3421 cgctgtcctg tcacactcgc taaaaacact aatcacacgg aaatctgttt tcaataaaaa
     3481 ctccaacttt aactcatttt caatttcaac tgaaagattt tttcattaga gaatgtctag
     3541 aactaggccc gggctacgta atacgactca cttaaggtgc cgctctagaa ctagtggatc
     3601 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     3661 cattatacga gacgtccagg ttgggatacc tgaaacaaaa cccatcgtac ggccaaggaa
     3721 gtctccaata actgtgatcc accacaagcg ccagggtttt cccagtcacg acgttgtaaa
     3781 acgacggcca gtcatgcata atccgcacgc atctggaata aggaagtgcc attccgcctg
     3841 acctttctgc attgttctaa aaactcacag gttatctcaa aaagataaat ttcaaagtga
     3901 gattttttta aaaaaattcc ggactgtttt ttgattttga tgtttttaat gaatttttaa
     3961 aattctttat tagtgcctga aattaacaat aattttaaag tttgtcacac tgcgaataaa
     4021 ttaatctcac atttcgtatg tttagaaaga tgcatgaaac tccgtcgttc ttatgaagtt
     4081 aattaagaca tggctaattg aaagcccaca tggtcatgaa aaagtaaaac ttacaacttt
     4141 ttactgaata ttaaccagtc tctataaata tcccagctta gccggaatag ggtcacgaaa
     4201 acatgaaaat aaagcttgaa cctattgtta tgattttcaa tgccaccttt tgcagtttaa
     4261 tatacactat tactacttct cagttcaagt tcacttctga gacggaacct ttattttcta
     4321 actttcttta atcttacttt ttatgagccg aatgttctat tttgtaaaat gatcagatac
     4381 aagcccaaag ctaattttgc caataagtgt tagtagatca attgaattac aatataatga
     4441 cctgggcgga atagattttt gcactgattt ttttccaagc ttactctatg aatattcata
     4501 gatcatcttc aaaggtagcg tgtgttgtag ttgtgttttg gcgggttcgt gttatttgac
     4561 tcagctcaag tagatgttgt agtcttccct caatcaacgc gtgggcttga ccctctttaa
     4621 attgagtgga aaccagccaa tctatttgaa atctaccccc gaaatgagat tcgacgatga
     4681 cacgacgtgt gtgttacgtg cgctgagaat ttaggaaaaa aaagataatc ttcttttcgt
     4741 tagctgcgcc acaacctgtg cagataaact ccgcacaatt gaagttctta ctggtcccac
     4801 ccgatttatt caattgtatt gtgtcttttt ttcttatttc tctacaactc taactttctt
     4861 caactattct ctacatcagt gcttttttcc accatgaaaa aagaaatgac gtcgttgttt
     4921 cttaggaggg gcggagcctc aactcaatgg tccgttcgaa tagatcccac ccctcatata
     4981 aataggactg aaaccaaaga agtttttaaa ttttcagctt accgtgtttc tcaattttca
     5041 aaatagtctt ttttgtgtct gaacgggctg atcaattatg tttttttttg aatgaaaaat
     5101 cttcctccca agccttaaaa ttccttccaa agatcgaaag ttatgtctat tcaagttact
     5161 tcttcttaga tcaaatatta aacaaaaaaa tacgccatca ttctctcaca tttagtaatt
     5221 caaaatttcg atttgttttc gaacttttga atttcccgcc taggaagtga catgagtgaa
     5281 atctcattag ataaatgtgc cactcaccga tgacactatt ttcaaataag gggggggggt
     5341 gtttcgaata ccttgtacac gcgtttccgc aaaaccacac accccgagtt cctcagcttc
     5401 ttcccctcga cccatgagcc acgtcaagat gtcagcgtgt cacttttcat tgcttcacct
     5461 tctacggtaa cgagaaaagg cggggaatcc aggatctggt gtgacttctt ccttcttcgt
     5521 atcttcctaa aaatgttcgt ttacttttta gcgagtgtca tttgatggga tgtcgttctt
     5581 gaggaaagag gctagaagct taatactctc atatttcata ttattggcat caagacgttt
     5641 attatttaac tacaataacg attgctttaa caactaaaaa taataatttc agggttagac
     5701 aaaaataact tcgtataaag tatcctatac gaagttatat gtccgaactc atcaaggaga
     5761 acatgcacat gaagctctac atggagggaa ccgtagacaa ccaccacttc aagtgcacct
     5821 ccgagggaga gggaaagcca tacgagggaa cccaaaccat gcgtatcaag gtcgtcgagg
     5881 gaggaccact cccattcgcc ttcgacatcc tcgccacctc cttcctctac gggtccaagg
     5941 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag accttcatca
     6001 accacaccca aggaatccca gacttcttca agcaatcctt cccagaggga ttcacctggg
     6061 agcgtgtcac cacctacgag gacggaggag tcctcaccgc cacccaagac acctccctcc
     6121 aagacggatg cctcatctac aacgtcaaga tccgtggagt caacttcacc tccaacggac
     6181 cagtcatgca aaagaagacc ctcggatggg agaccttcac cgagaccctc tacccagccg
     6241 acggaggact agagggacgt aacgacatgg ccctcaagct cgtcggaggc tcccacctca
     6301 tcgccaacgc caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     6361 ttcagaccac ctaccgttcc aagaagccag ccaagaacct caagatgcca ggagtctact
     6421 acgttgacta ccgtctagag cgtatcaagg aggccaacaa cgagacctac gtcgagcaac
     6481 acgaggtcgc cgtcgcccgt tactgcgacc tcccatccaa gctcggacac aagctcaact
     6541 aagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt
     6601 gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt
     6661 atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct
     6721 gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg
     6781 agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag
     6841 gaaactttat tgtgccgcca agaaaaaagt ctcagccggc ataacttcgt ataaagtatc
     6901 ctatacgaag ttatatgagc agcatcacct gtgatcccgc catctacggc gaatggagca
     6961 gggagaacca gttctgcgtg gagaagagcc tgatcaccct ggacggcatc aagtacgtcc
     7021 agctggtgat ggccgtcgtg agcgcctgtc aggtgttctt catggtgacc agagccccca
     7081 aggtgccctg ggaagccatc tacctgccca ccaccgagat gatcacctat tccctggcct
     7141 tcaccggaaa cggctacatc agagtggcca atggcaagta cctgccctgg gccagaatgg
     7201 ccagctggct gtgcacctgc cctatcatgc tgggcctggt gagcaatatg gccctcgtga
     7261 agtacaagag catccccctc aaccctatga tgatcgccgc ctccagcatc tgcaccgtgt
     7321 tcggcatcac cgcctccgtg gtgctagacc cgctgcacgt gtggctgtac tgtttcatca
     7381 gcagcatctt cttcatcttc gagatggtgg tggccttcgc cattttcgcc attaccatcc
     7441 acgatttcca gaccatcggc tcccccatgt ccctgaaggt ggtggagagg ctgaagctga
     7501 tgaggatcgt gttctacgtg agctggatgg cctaccctat cctgtggagc ttctccagca
     7561 ccggcgcctg catcatgagc gagaacacca gcagcgtgct gtacctgctg ggcgacgctc
     7621 tgtgcaagaa cacctacggc atcctgctgt gggctacaac ctggggcctg ctgaacggca
     7681 agtgggacag ggattacgtg aagggcagga acgtggacgg caccctgatg cctgagtacg
     7741 agcaggacct ggagaagggc aacaccgaga ggtacgagga cgccagagcc ggcgagacct
     7801 aagctgtctc atcctacttt cacctagtta actgcttgtc ttaaaatcta tgcttctctt
     7861 tagtatctaa aattttccta gaagcttaca agtatataaa tggtctcttc tcaataaagg
     7921 ttgtatattt attcatctta ttgaatctgc catttcctcg tttttgcgag tttatatacc
     7981 ttccaatttt ctttctattg tattttcaac ttctaatttt aattcaggga aactgctgta
     8041 ccggtagaaa aaatggtcga ctcatcacgt agaaaatgga acaaagctgg tcacgctgtc
     8101 cgtgccatcg gacgtctctc ctctccagtc gtctccgagc gtatgtaccc agaggacgga
     8161 gccctcaagt ccgagatcaa gaagggactc cgtctcaagg acggaggaca ctacgccgcc
     8221 gaggtcaaga ccacctacaa ggccaagaag ccagtccaac tcccaggagc ctacatcgtc
     8281 gacatcaagc tcgacatcgt ctcccacaac gaggactaca ccatcgtcga gcaatgcgag
     8341 cgtgccgagg gacgtcactc caccggagga atggacgagc tctacaaggg aggaaccgga
     8401 ggttccctcg tctccaaggg agaggaggac aacatggcca tcatcaagga gttcatgcgt
     8461 ttcaaggtcc acatggaggg ttccgtcaac ggacacgagt tcgagatcga gggagaggga
     8521 gagggacgtc catacgaggc cttccaaacc gccaagctca aggtcaccaa gggaggacca
     8581 ctcccattcg cctgggacat cctctcccca caattcatgt acggctccaa ggcctacatc
     8641 aagcacccag ccgacatccc agactacttc aagctctcct tcccagaggg attccgttgg
     8701 gagcgtgtca tgaacttcga ggacggagga atcatccacg tcaaccaaga ctcctccctc
     8761 caagacggag tcttcatcta caaggtcaag ctccgtggaa ccaacttccc accagacgga
     8821 ccagtcatgc aaaagaagac catgggatgg gaggccaccc gtgacgacct caccgaggag
     8881 caaatcgccg agttcaagga ggccttctcc ctcttcgaca aggacggaga cggaaccatc
     8941 accaccaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     9001 gagctcggaa ccgtcttccg ttccctcgga caaaacccaa ccgaggccga gctccaagac
     9061 atgatcaacg aggtcgacgc cgacggagac ggaaccttcg acttcccaga gttcctcacc
     9121 atgatggccc gtaagatgaa cgacaccgac tccgaggagg agatccgtga ggccttccgt
     9181 gtcttcgaca aggacggaaa cggatacatc ggagccgccg agctccgtca cgtcatgacc
     9241 gacctcggag agaagctcac cgacgaggag gtcgacgaga tgatccgtgt cgccgacatc
     9301 gacggagacg gacaagtcaa ctacgaggag ttcgtccaaa tgatgaccgc caagtaaggt
     9361 accgaattcg ctagccggcc atacaagtaa tccggatgat cgacgccaac gtcgttgaat
     9421 tttcaaattt taaatactga atatttgttt tttttcctat tatttattta ttctctttgt
     9481 gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt tttttttctc
     9541 tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt
     9601 atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca
     9661 aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata
     9721 tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa actagtaaaa
     9781 aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttgggccc tctgacttct
     9841 cccgggcaaa ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg
     9901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt
     9961 ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg
    10021 tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggactagt gagtcgtatt
    10081 ataagtgcaa gtaagatcag tgtttgtttc gatatcagtc tgtttcgtaa cggtcttctg
    10141 tataactaca aaaaagaata aaaaaccgta tcacaactga gaaaaaagac aaaacttatt
    10201 gaacagtgtt cctttcaaaa aattattttg taaaaaatcg agtttttctc caaaataacg
    10261 cacgcaatca aaaacaagaa aactaacgtg ttctcctggg cgtggaactc cttatcaaga
    10321 ttaccattat tgtctgtcag aattgatatt tcaattattt catatctaga caaacccggg
    10381 tgtcttgcat ttattagtac tgtgagttta catacaaacg aggcttactc agataacata
    10441 gagtcacctc taattagcaa aaacccattt ctaattagct atttcgacca gttctaaagt
    10501 caccgtaatt agaaatggtt tttgtagctt ttctaattag aaatgacccc gagataagtg
    10561 aaatatggca ttttctgggt tttctagtaa gaaatgatcc cgcacgtagt gattccgcgt
    10621 tacaagccaa attcaaatta aaaaagtata ttacacaatt caagttacat tattttcagg
    10681 aaacaatggc gaagagcaaa gcagagattc aaaaggagga aagagacaac agatatgaac
    10741 gaagacttct tgaaaagaga aagagacatg aggtgagtaa ccatgataat aaataatcaa
    10801 gtaagggcta tagatttata aattctggag ttaacagttt cgaaaaaaag tagttttcat
    10861 atgtgcgaag gaatatcaaa aataggggaa tagcctaaac gagtgagaaa attaaatgtt
    10921 tgttattttt tcaaaaatgt ctgcagggcc gttatccttt caaatgcact ctatcaaaca
    10981 tagatcatat ccatcatatc ttatccagaa tttatatcat atccaagatt gagagcgttt
    11041 caaggaaaat ctcaaacaac catacaattt tgtagatcga caacaaggcc tccacttctt
    11101 catcgatggc ctctgcagca cctgcaccac caagtttgta tttttcatat taatataaag
    11161 tgagccacgc atagtcgttt ttaggatttt tgccagatcg atgtcgcgca aacagactgt
    11221 ttttttttct ttgaaagccg tgcgtgacat gggccacgga ttgtaacgcc gcgctggatg
    11281 gataaaaatt tattcagttt tttagccttt acaaaatatg gttcgactac tttctttatt
    11341 tttacatttt tataggtgct cattgagctg agatattttg ggtataatcg gaaaatcgct
    11401 gattttggtc atttttctgc actaaagatt acacaaaaag ttcagtattt agattcattg
    11461 ttgaaaattg gggatgatct acagatacat atcgccgtgt tcaaaaacac acgtgctgtg
    11521 acgtcgtgac tggggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11581 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11641 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    11701 tttcacaccg catatggtgc actctcagtg caatctgctc tgatgccgca tagttaagcc
    11761 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
    11821 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    11881 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
    11941 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
    12001 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    12061 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    12121 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    12181 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    12241 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    12301 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    12361 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    12421 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    12481 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    12541 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    12601 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    12661 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    12721 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    12781 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    12841 ggtcagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    12901 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    12961 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    13021 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    13081 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    13141 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    13201 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    13261 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    13321 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    13381 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    13441 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    13501 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    13561 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    13621 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    13681 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    13741 tacggttcct ggtcttttgc tggccttttg ctcacatgtt ctttcc
//
