LOCUS       sequence-404772-        9340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentivirus backbone cloning vector and template for barcoded 
            non-neutralizable standard production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW,
            Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom 
            JD
  TITLE     A pseudovirus system enables deep mutational scanning of the full 
            SARS-CoV-2 spike.
  JOURNAL   Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 
            10.1016/j.cell.2023.02.001. Epub 2023 Feb 13.
  PUBMED    36868218
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.02.001"; journalName: "Cell"; date: 
            "2023-03-16- 16"; volume: "186"; issue: "6"; pages: "1263-1278.e20"
COMMENT     Sequence Label: pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    38..56
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    74..92
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             269..979
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(414..432)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     682..701
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     enhancer        1069..1372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1373..1576
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1527..1547
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1573..1597
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1648..1657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1654..2346
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     CDS             2365..2418
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2419..3018
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2419..2438)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2915..2935
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3026..3614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3079..3099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3497..3508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3688..4321
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4370..4388)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4456..4560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4561..4629,4630..5421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4779..4798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5592..6180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6334..6351
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6591..6612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6627..6632,6633..6650,6651..6657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6670..6692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6689..6705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6689..6705
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6755..6774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6966..7599
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7646..7771
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8268..8501
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8686..8730
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9023..9140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9190..9340,1..217)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    9198..9216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9234..9252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9270..9288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9306..9324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
       61 atatccagtt tactccctat cagtgataga gaacgtataa gctttgctta tgtaaaccag
      121 ggcgcctata aaagagtgct gattttttga gtaaacttca attccacaac acttttgtct
      181 tataccaact ttccgtacca cttcctaccc tcgtaaagaa ttcgcggccg cacgcgcagc
      241 cgagccacat cgctcaacgc gtgccaccat ggtcagcaag ggcgaggagg ataacatggc
      301 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      361 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      421 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcaattcat
      481 gtatggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      541 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      601 cgtgacccag gactcctcct tacaagacgg cgagttcatc tacaaagtga agctgcgcgg
      661 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      721 ctccgagcgg atgtacccag aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      781 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      841 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
      901 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      961 ggacgagctg tacaagtaag gcgcgcctag ttggatcaca ctctttccct acacgacgct
     1021 cttccgatct tctagattcg agagagaaat gttcctagtg gagttccgcg ttacataact
     1081 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1141 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1201 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1261 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1321 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1381 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1441 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1501 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1561 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1621 ttttgacctc catagaagac accggcggcc gccatggccc agtccaagca cggcctgacc
     1681 aaggagatga ccatgaagta ccgcatggag ggctgcgtgg acggccacaa gttcgtgatc
     1741 accggcgagg gcatcggcta ccccttcaag ggcaagcagg ccatcaacct gtgcgtggtg
     1801 gagggcggcc ccttgccctt cgccgaggac atcttgtccg ccgccttcat gtacggcaac
     1861 cgcgtgttca ccgagtaccc ccaggacatc gtcgactact tcaagaactc ctgccccgcc
     1921 ggctacacct gggaccgctc cttcctgttc gaggacggcg ccgtgtgcat ctgcaacgcc
     1981 gacatcaccg tgagcgtgga ggagaactgc atgtaccacg agtccaagtt ctacggcgtg
     2041 aacttccccg ccgacggccc cgtgatgaag aagatgaccg acaactggga gccctcctgc
     2101 gagaagatca tccccgtgcc caagcagggc atcttgaagg gcgacgtgag catgtacctg
     2161 ctgctgaagg acggtggccg cttgcgctgc cagttcgaca ccgtgtacaa ggccaagtcc
     2221 gtgccccgca agatgcccga ctggcacttc atccagcaca agctgacccg cgaggaccgc
     2281 agcgacgcca agaaccagaa gtggcacctg accgagcacg ccatcgcctc cggctccgcc
     2341 ttgcccgctg gatccggctc cggcgagggc aggggaagtc ttctaacatg cggggacgtg
     2401 gaggaaaatc ccggcccaat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     2461 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     2521 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     2581 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     2641 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     2701 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     2761 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     2821 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     2881 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     2941 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3001 cgtaagcccg gtgcctgagg atcctaatca acctctggat tacaaaattt gtgaaagatt
     3061 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3421 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3601 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     3661 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagacaag
     3721 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     3781 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     3841 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     3901 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     3961 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     4021 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     4081 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     4141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4321 agtcccgggc gattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     4381 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4441 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4501 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4561 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4621 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4681 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4741 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4801 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4861 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4921 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4981 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5041 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5101 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5161 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5221 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5281 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5341 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5401 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5461 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5521 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5581 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5641 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5701 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5761 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5821 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5881 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5941 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6001 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6121 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6181 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6241 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6301 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6361 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     6421 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6481 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     6541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6661 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     6721 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6781 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     6841 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     6901 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     6961 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     7021 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7081 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7141 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7201 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7261 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     7321 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7381 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7561 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7621 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7681 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7741 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7801 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7861 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7921 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7981 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8041 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8101 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8161 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8221 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8281 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8341 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8401 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8461 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8521 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     8581 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     8641 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     8701 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     8761 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     8821 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     8881 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     8941 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
     9001 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg cacagtgcag
     9061 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9121 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
     9181 gtctctctcg agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat
     9241 cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag
     9301 tttactccct atcagtgata gagaacgtat gaccagttta
//
