LOCUS       sequence-404774-       13843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 
            3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, 
            Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, 
            Gonzalez-Bolivar S, Hurth C, Krieg M
  TITLE     Neural engineering with photons as synaptic transmitters.
  JOURNAL   Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.
  PUBMED    37024651
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9."
COMMENT     Sequence Label: pNMSB84
FEATURES             Location/Qualifiers
     source          1..13843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1553..1564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3569..3585
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3569..3585
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3586..3632
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3746..3768
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3760..3777
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3761..3777
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4568..4579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5690..5723
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     terminator      6533..6856
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6866..6899
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(9948..9964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9948..9964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11890..11912
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11950..11968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12036..12140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12141..12209,12210..13001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12359..12378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13172..13760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13661..13680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accagcggat aacaatttca
       61 cagggccctg tcggattatg ggctcttctt ttatcctcta ctttctttct agaaaatgca
      121 aatgttgagt cagaaattgc tgaatggagt acaactgcaa tgggtttagc ttatgtttct
      181 ggaacaatta ctggtggaat tgtgattgaa aagtaaggtt atatttcatt tcaataaatg
      241 ttaccacagt ataacaaaaa caagttacat tgttcagagt atgtctgaat gtattattat
      301 tattgtattt gtgcatcagt actgtagttg tactgaagaa gggtttaaaa taatctcaac
      361 gtttttaaga cgagttagtg agaatacggt cgcagttagg atgtttggac atagttttat
      421 tagataatcg tgaactaact cactataaaa acccgctaac tcattgaata agatagtttt
      481 aaacgttcaa aatttgaaga caaaattgta tgaataagtt tacagagttg gtcgacgcaa
      541 gcttctcctt ctcttcacat ttctcaataa tttagctcta attgcattcg tattcttcgc
      601 aaaaatacgt attctgattg acccgatgaa atacggatgc ctagttgcat tgatcatcta
      661 cggttatact tatgggtgag gtttttcaaa tatattttga tttaatcgaa taatgaaatt
      721 tcagaactgg agttggtcca atttcgtggt ttatatcctc tgaactggta cctcaaaaac
      781 atcgtagtat tgctcaatct gtggcatatt caatgaatac tgtaatggtg gttatcagta
      841 catttacagt attacctcta tattccgtta ttggtagtta tgctttcctt attctatatt
      901 caattccttc atttataagt atgcttatat tatttcgcta cttaccggaa accaaaggac
      961 gagagattca tgatattgtg aatgaactta aaaaaaaatg aaaacctttg aaaacgttca
     1021 tacggccgaa attttacttg atcgagccaa ttgaactttt gttaagactt acagaaaagt
     1081 gagagatttt gttacaaaaa agtgagaaaa aagcgaaaat gaacaggaaa aagaagaaaa
     1141 atacacccga agtatcatat aaaaattaaa aagattgttt gacctggcgg aacttttttg
     1201 caaaaactct gaatatccat ggcacatgct tcgtgcaaaa caggatccaa atgaatatca
     1261 acaagagatt cttgaagacg acgagccact tgctcagagc attgcttatc tcgaatgaga
     1321 cccttgttaa aatcattttt caaacattat atgacattgt cgaaatgtcc tcctgattcc
     1381 atgatggtag caaactcact tcgtctttga gccaatttat ccaagtcctt gtaaaaagta
     1441 ttcgaaaatt gttaacggat aaaatgttta ttataatatc aaaaacaatt tgtcagttga
     1501 ccactttttg atatatttga cagaaaccgg gatgaattgg ctcaaagtta gggcgccctt
     1561 ctattacagg gtttctgata acaaaccggt tattaactcc caacaaggga tgatttcaat
     1621 tcatcatgct caaattgacc caaattaagt tacatgacaa attcatcgcc cttttcaact
     1681 cttcttggct catcatctgt tattctgttc ctattctgtc tgcaccctat acccctttgc
     1741 atactctctc gtcattcctt tcttggatag tgtcttcttc tccccagctc tgctacttct
     1801 atgacttgcg cccgctgctt ttccgctcgt tcctctcctc tctgacgtct tcttctgctt
     1861 cttttctcct tcctttttcc cttctcaatc ttcctctttt tcccatttct gtcaatcatt
     1921 cgaagaagaa gaagaagaag accctcattc atttcatttt tttttctgtc ggtgtgtgct
     1981 gctcggttaa gagtgagctc tctattccac gtcttcttct ttttcttctt cgattcgaat
     2041 caatcactcc accaaaacgc attcgttttt gggattcacc ccgcggttcg caataggttt
     2101 ctttttcaaa ttattagcgt tataaataga aaaatggtgg agtttcaata aaaatgataa
     2161 tttacaaagt gtattttgat tacatgtact caaaaaggtt gaaatttttc ataccagttt
     2221 tccggaaatc catctgatat cattatcgta tttttctttt taaaaatgtt ttcaaaaaaa
     2281 aacaaaatat agctggttat ttggcaccct ctaattacca ttttcctgtc acaccacact
     2341 cttttccttc ttccacttct ttccgttttc agccgcttcc aaccaaaccg atatgaaagc
     2401 cgagcaacaa cgatcgattc cacccggctc ggcgaccttc ccgtcgcagg tgagactcag
     2461 aaaactagag aaaccgctca actaactctt gatatccgat ttcattcttt tctcttttct
     2521 ttttgttgaa cttttccata tttccagatg ccacggccac caccaagcac cgaacaagga
     2581 atcacaacgg aatcggagct tgcgaagaaa gctcaaatca ctccgaacga cgttttagca
     2641 cttccgggaa tcactcaagg tatcttgctc cctttggatt tcagtttaaa acataaattt
     2701 aggattctta tgctccccat ctgcgaacat ctataacatc gagttcacca agttccaaat
     2761 ccgtgatctg gacactgagc aagtgctttt cgagatcgcc aaaccggaga acgatcagga
     2821 gaatgatgag tcgccacagg agtcggcaag atacgtgcgt tatagatttg ctccaaactt
     2881 tttgaaactc aaaacggtcg gagcaactgt ggaattcaaa gtaggagaca tcccaatcca
     2941 tcatttccga atgatcgaac gtcacttctt caaagatcgc cttctgaagt gttttgactt
     3001 tgaattcgga ttctgtattc cgaattcacg aaacaactgt gaacatatct atgagttccc
     3061 tcaactctct caacaactca gtgagtcatt attctaaaaa gtacaattca aaagactaat
     3121 ctctttttca gtggacgaca tgatcaacaa tccaaacgag actcgttctg acagcttcta
     3181 tttcgtcgat aacaaactcg tcatgcacaa caaagccgac tactcatatg atgcataaat
     3241 atttaataca aaaatgttct ggataattat tctgtcgaat agaaaaaaaa ctccaaatgt
     3301 gattaaattc caataattcc tgtctagttt gttccttccc cttccccttc tcatgttcaa
     3361 tgcattccta agcttttcag ttcccccctt gtttctatat tttttcgctg tcctgtcaca
     3421 ctcgctaaaa acactaatca cacggaaatc tgttttcaat aaaaactcca actttaactc
     3481 attttcaatt tcaactgaaa gattttttca ttagagaatg tctagaacta ggcccgggct
     3541 acgtaatacg actcacttaa ggtgccgctc tagaactagt ggatccaaat aaaacgaaag
     3601 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta tacgagacgt
     3661 ccaggttggg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     3721 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     3781 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacctt tctgcattgt
     3841 tctaaaaact cacaggttat ctcaaaaaga taaatttcaa agtgagattt ttttaaaaaa
     3901 attccggact gttttttgat tttgatgttt ttaatgaatt tttaaaattc tttattagtg
     3961 cctgaaatta acaataattt taaagtttgt cacactgcga ataaattaat ctcacatttc
     4021 gtatgtttag aaagatgcat gaaactccgt cgttcttatg aagttaatta agacatggct
     4081 aattgaaagc ccacatggtc atgaaaaagt aaaacttaca actttttact gaatattaac
     4141 cagtctctat aaatatccca gcttagccgg aatagggtca cgaaaacatg aaaataaagc
     4201 ttgaacctat tgttatgatt ttcaatgcca ccttttgcag tttaatatac actattacta
     4261 cttctcagtt caagttcact tctgagacgg aacctttatt ttctaacttt ctttaatctt
     4321 actttttatg agccgaatgt tctattttgt aaaatgatca gatacaagcc caaagctaat
     4381 tttgccaata agtgttagta gatcaattga attacaatat aatgacctgg gcggaataga
     4441 tttttgcact gatttttttc caagcttact ctatgaatat tcatagatca tcttcaaagg
     4501 tagcgtgtgt tgtagttgtg ttttggcggg ttcgtgttat ttgactcagc tcaagtagat
     4561 gttgtagtct tccctcaatc aacgcgtggg cttgaccctc tttaaattga gtggaaacca
     4621 gccaatctat ttgaaatcta cccccgaaat gagattcgac gatgacacga cgtgtgtgtt
     4681 acgtgcgctg agaatttagg aaaaaaaaga taatcttctt ttcgttagct gcgccacaac
     4741 ctgtgcagat aaactccgca caattgaagt tcttactggt cccacccgat ttattcaatt
     4801 gtattgtgtc tttttttctt atttctctac aactctaact ttcttcaact attctctaca
     4861 tcagtgcttt tttccaccat gaaaaaagaa atgacgtcgt tgtttcttag gaggggcgga
     4921 gcctcaactc aatggtccgt tcgaatagat cccacccctc atataaatag gactgaaacc
     4981 aaagaagttt ttaaattttc agcttaccgt gtttctcaat tttcaaaata gtcttttttg
     5041 tgtctgaacg ggctgatcaa ttatgttttt ttttgaatga aaaatcttcc tcccaagcct
     5101 taaaattcct tccaaagatc gaaagttatg tctattcaag ttacttcttc ttagatcaaa
     5161 tattaaacaa aaaaatacgc catcattctc tcacatttag taattcaaaa tttcgatttg
     5221 ttttcgaact tttgaatttc ccgcctagga agtgacatga gtgaaatctc attagataaa
     5281 tgtgccactc accgatgaca ctattttcaa ataagggggg ggggtgtttc gaataccttg
     5341 tacacgcgtt tccgcaaaac cacacacccc gagttcctca gcttcttccc ctcgacccat
     5401 gagccacgtc aagatgtcag cgtgtcactt ttcattgctt caccttctac ggtaacgaga
     5461 aaaggcgggg aatccaggat ctggtgtgac ttcttccttc ttcgtatctt cctaaaaatg
     5521 ttcgtttact ttttagcgag tgtcatttga tgggatgtcg ttcttgagga aagaggctag
     5581 aagcttaata ctctcatatt tcatattatt ggcatcaaga cgtttattat ttaactacaa
     5641 taacgattgc tttaacaact aaaaataata atttcagggt tagacaaaaa taacttcgta
     5701 taaagtatcc tatacgaagt tatatgtccg aactcatcaa ggagaacatg cacatgaagc
     5761 tctacatgga gggaaccgta gacaaccacc acttcaagtg cacctccgag ggagagggaa
     5821 agccatacga gggaacccaa accatgcgta tcaaggtcgt cgagggagga ccactcccat
     5881 tcgccttcga catcctcgcc acctccttcc tctacgggtc caaggtaagt ttaaacatat
     5941 atatactaac taaccctgat tatttaaatt ttcagacctt catcaaccac acccaaggaa
     6001 tcccagactt cttcaagcaa tccttcccag agggattcac ctgggagcgt gtcaccacct
     6061 acgaggacgg aggagtcctc accgccaccc aagacacctc cctccaagac ggatgcctca
     6121 tctacaacgt caagatccgt ggagtcaact tcacctccaa cggaccagtc atgcaaaaga
     6181 agaccctcgg atgggagacc ttcaccgaga ccctctaccc agccgacgga ggactagagg
     6241 gacgtaacga catggccctc aagctcgtcg gaggctccca cctcatcgcc aacgccaagg
     6301 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag accacctacc
     6361 gttccaagaa gccagccaag aacctcaaga tgccaggagt ctactacgtt gactaccgtc
     6421 tagagcgtat caaggaggcc aacaacgaga cctacgtcga gcaacacgag gtcgccgtcg
     6481 cccgttactg cgacctccca tccaagctcg gacacaagct caactaagat aaatgcaaaa
     6541 tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa aaaattctct
     6601 cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag
     6661 tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt actttcacaa
     6721 tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa aatgcgagcg
     6781 ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac tttattgtgc
     6841 cgccaagaaa aaagtctcag ccggcataac ttcgtataaa gtatcctata cgaagttata
     6901 tggattatgg aggcgccctg agtgccgttg ggcgcgagct gctatttgta acgaacccag
     6961 tagtcgtcaa tggctctgta cttgtgcctg aggaccagtg ttactgcgcg ggctggattg
     7021 agtcgcgtgg cacaaacggt gcccaaacgg cgtcgaacgt gctgcaatgg cttgctgctg
     7081 gcttctccat cctactgctt atgttttacg cctaccaaac atggaagtca acctgcggct
     7141 gggaggagat ctatgtgtgc gctatcgaga tggtcaaggt gattctcgag ttcttcttcg
     7201 agtttaagaa cccgtccatg ctgtatctag ccacaggcca ccgcgtccag tggttgcgtt
     7261 acgccgagtg gcttctcacc tgcccggtca ttctcattcg cctgtcaaac ctgacgggct
     7321 tgtccaacga ctacagcagg cgcaccatgg gtctgcttgt gtcttgcatc ggaaccattg
     7381 tgtggggcgc cacttccgcc atggccaccg gatacgtcaa ggtcatcttc ttctgcctgg
     7441 gtctgtgtta tggtgctaac acgttctttc acgctgccaa ggcctacatc gagggttacc
     7501 acaccgtgcc gaagggccgg tgtcgccagg tggtgactgg catggcttgg ctcttcttcg
     7561 tatcatgggg tatgttcccc atcctgttca tcctcggtcc cgagggtttc ggtgtcctga
     7621 gcgtgtacgg ttccaccgtc ggccacacca tcattgacct gatgtcgaag aactgctggg
     7681 gtctgctcgg ccactacctg cgcgtgctga tccacgagca tatcctcatc cacggcgaca
     7741 ttcgcaagac caccaaattg aacattggtg gcactgagat tgaggtcgag acgctggtgg
     7801 aggacgaagc cgaagctggt gcagtacctg ccgctgccac ctaagctgtc tcatcctact
     7861 ttcacctagt taactgcttg tcttaaaatc tatgcttctc tttagtatct aaaattttcc
     7921 tagaagctta caagtatata aatggtctct tctcaataaa ggttgtatat ttattcatct
     7981 tattgaatct gccatttcct cgtttttgcg agtttatata ccttccaatt ttctttctat
     8041 tgtattttca acttctaatt ttaattcagg gaaactgctg taccggtaga aaaaatggtc
     8101 gactcatcac gtagaaaatg gaacaaagct ggtcacgctg tccgtgccat cggacgtctc
     8161 tcctctccag tcgtctccga gcgtatgtac ccagaggacg gagccctcaa gtccgagatc
     8221 aagaagggac tccgtctcaa ggacggagga cactacgccg ccgaggtcaa gaccacctac
     8281 aaggccaaga agccagtcca actcccagga gcctacatcg tcgacatcaa gctcgacatc
     8341 gtctcccaca acgaggacta caccatcgtc gagcaatgcg agcgtgccga gggacgtcac
     8401 tccaccggag gaatggacga gctctacaag ggaggaaccg gaggttccct cgtctccaag
     8461 ggagaggagg acaacatggc catcatcaag gagttcatgc gtttcaaggt ccacatggag
     8521 ggttccgtca acggacacga gttcgagatc gagggagagg gagagggacg tccatacgag
     8581 gccttccaaa ccgccaagct caaggtcacc aagggaggac cactcccatt cgcctgggac
     8641 atcctctccc cacaattcat gtacggctcc aaggcctaca tcaagcaccc agccgacatc
     8701 ccagactact tcaagctctc cttcccagag ggattccgtt gggagcgtgt catgaacttc
     8761 gaggacggag gaatcatcca cgtcaaccaa gactcctccc tccaagacgg agtcttcatc
     8821 tacaaggtca agctccgtgg aaccaacttc ccaccagacg gaccagtcat gcaaaagaag
     8881 accatgggat gggaggccac ccgtgacgac ctcaccgagg agcaaatcgc cgagttcaag
     8941 gaggccttct ccctcttcga caaggacgga gacggaacca tcaccaccaa ggtaagttta
     9001 aacatgattt tactaactaa ctaatctgat ttaaattttc aggagctcgg aaccgtcttc
     9061 cgttccctcg gacaaaaccc aaccgaggcc gagctccaag acatgatcaa cgaggtcgac
     9121 gccgacggag acggaacctt cgacttccca gagttcctca ccatgatggc ccgtaagatg
     9181 aacgacaccg actccgagga ggagatccgt gaggccttcc gtgtcttcga caaggacgga
     9241 aacggataca tcggagccgc cgagctccgt cacgtcatga ccgacctcgg agagaagctc
     9301 accgacgagg aggtcgacga gatgatccgt gtcgccgaca tcgacggaga cggacaagtc
     9361 aactacgagg agttcgtcca aatgatgacc gccaagtaag gtaccgaatt cgctagccgg
     9421 ccatacaagt aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact
     9481 gaatatttgt tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc
     9541 taaaaaatta attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg
     9601 tattccgctc ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt
     9661 cattaggacg tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga
     9721 aataaggaca cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag
     9781 aaaacataaa aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag
     9841 gcgaacgatg agaattgtcc tcgcttgggc cctctgactt ctcccgggca aaggctaggt
     9901 ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt
     9961 cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc
    10021 tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt
    10081 gagcaaaagg ccagcaaaag gccaggacta gtgagtcgta ttataagtgc aagtaagatc
    10141 agtgtttgtt tcgatatcag tctgtttcgt aacggtcttc tgtataacta caaaaaagaa
    10201 taaaaaaccg tatcacaact gagaaaaaag acaaaactta ttgaacagtg ttcctttcaa
    10261 aaaattattt tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat caaaaacaag
    10321 aaaactaacg tgttctcctg ggcgtggaac tccttatcaa gattaccatt attgtctgtc
    10381 agaattgata tttcaattat ttcatatcta gacaaacccg ggtgtcttgc atttattagt
    10441 actgtgagtt tacatacaaa cgaggcttac tcagataaca tagagtcacc tctaattagc
    10501 aaaaacccat ttctaattag ctatttcgac cagttctaaa gtcaccgtaa ttagaaatgg
    10561 tttttgtagc ttttctaatt agaaatgacc ccgagataag tgaaatatgg cattttctgg
    10621 gttttctagt aagaaatgat cccgcacgta gtgattccgc gttacaagcc aaattcaaat
    10681 taaaaaagta tattacacaa ttcaagttac attattttca ggaaacaatg gcgaagagca
    10741 aagcagagat tcaaaaggag gaaagagaca acagatatga acgaagactt cttgaaaaga
    10801 gaaagagaca tgaggtgagt aaccatgata ataaataatc aagtaagggc tatagattta
    10861 taaattctgg agttaacagt ttcgaaaaaa agtagttttc atatgtgcga aggaatatca
    10921 aaaatagggg aatagcctaa acgagtgaga aaattaaatg tttgttattt tttcaaaaat
    10981 gtctgcaggg ccgttatcct ttcaaatgca ctctatcaaa catagatcat atccatcata
    11041 tcttatccag aatttatatc atatccaaga ttgagagcgt ttcaaggaaa atctcaaaca
    11101 accatacaat tttgtagatc gacaacaagg cctccacttc ttcatcgatg gcctctgcag
    11161 cacctgcacc accaagtttg tatttttcat attaatataa agtgagccac gcatagtcgt
    11221 ttttaggatt tttgccagat cgatgtcgcg caaacagact gttttttttt ctttgaaagc
    11281 cgtgcgtgac atgggccacg gattgtaacg ccgcgctgga tggataaaaa tttattcagt
    11341 tttttagcct ttacaaaata tggttcgact actttcttta tttttacatt tttataggtg
    11401 ctcattgagc tgagatattt tgggtataat cggaaaatcg ctgattttgg tcatttttct
    11461 gcactaaaga ttacacaaaa agttcagtat ttagattcat tgttgaaaat tggggatgat
    11521 ctacagatac atatcgccgt gttcaaaaac acacgtgctg tgacgtcgtg actggggaaa
    11581 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    11641 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    11701 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    11761 gcactctcag tgcaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
    11821 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
    11881 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
    11941 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
    12001 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    12061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    12121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    12181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    12241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    12301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    12361 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    12421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    12481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    12541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    12601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    12661 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    12721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    12781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    12841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggtcagat ggtaagccct
    12901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    12961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    13021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    13081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    13141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    13201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    13261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    13321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    13381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    13441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    13501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    13561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    13621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    13681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    13741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggtctttt
    13801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tga
//
