LOCUS       sequence-404775-       13948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 
            3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Porta-de-la-Riva M, Gonzalez AC, Sanfeliu-Cerdan N, Karimi S, 
            Malaiwong N, Pidde A, Morales-Curiel LF, Fernandez P, 
            Gonzalez-Bolivar S, Hurth C, Krieg M
  TITLE     Neural engineering with photons as synaptic transmitters.
  JOURNAL   Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.
  PUBMED    37024651
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9."
COMMENT     Sequence Label: pNMSB104
FEATURES             Location/Qualifiers
     source          1..13948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(791..802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2807..2823
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2807..2823
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2824..2870
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2984..3006
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2998..3015
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2999..3015
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3806..3817)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4928..4961
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     terminator      5771..6094
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6104..6137
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(9291..9307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9291..9307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11233..11255
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11293..11311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11379..11483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11484..11552,11553..12344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11702..11721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12515..13103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13004..13023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aactggtacc tcaaaaacat cgtagtattg ctcaatctgt ggcatattca atgaatactg
       61 taatggtggt tatcagtaca tttacagtat tacctctata ttccgttatt ggtagttatg
      121 ctttccttat tctatattca attccttcat ttataagtat gcttatatta tttcgctact
      181 taccggaaac caaaggacga gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa
      241 aacctttgaa aacgttcata cggccgaaat tttacttgat cgagccaatt gaacttttgt
      301 taagacttac agaaaagtga gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga
      361 acaggaaaaa gaagaaaaat acacccgaag tatcatataa aaattaaaaa gattgtttga
      421 cctggcggaa cttttttgca aaaactctga atatccatgg cacatgcttc gtgcaaaaca
      481 ggatccaaat gaatatcaac aagagattct tgaagacgac gagccacttg ctcagagcat
      541 tgcttatctc gaatgagacc cttgttaaaa tcatttttca aacattatat gacattgtcg
      601 aaatgtcctc ctgattccat gatggtagca aactcacttc gtctttgagc caatttatcc
      661 aagtccttgt aaaaagtatt cgaaaattgt taacggataa aatgtttatt ataatatcaa
      721 aaacaatttg tcagttgacc actttttgat atatttgaca gaaaccggga tgaattggct
      781 caaagttagg gcgcccttct attacagggt ttctgataac aaaccggtta ttaactccca
      841 acaagggatg atttcaattc atcatgctca aattgaccca aattaagtta catgacaaat
      901 tcatcgccct tttcaactct tcttggctca tcatctgtta ttctgttcct attctgtctg
      961 caccctatac ccctttgcat actctctcgt cattcctttc ttggatagtg tcttcttctc
     1021 cccagctctg ctacttctat gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc
     1081 tgacgtcttc ttctgcttct tttctccttc ctttttccct tctcaatctt cctctttttc
     1141 ccatttctgt caatcattcg aagaagaaga agaagaagac cctcattcat ttcatttttt
     1201 tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc tattccacgt cttcttcttt
     1261 ttcttcttcg attcgaatca atcactccac caaaacgcat tcgtttttgg gattcacccc
     1321 gcggttcgca ataggtttct ttttcaaatt attagcgtta taaatagaaa aatggtggag
     1381 tttcaataaa aatgataatt tacaaagtgt attttgatta catgtactca aaaaggttga
     1441 aatttttcat accagttttc cggaaatcca tctgatatca ttatcgtatt tttcttttta
     1501 aaaatgtttt caaaaaaaaa caaaatatag ctggttattt ggcaccctct aattaccatt
     1561 ttcctgtcac accacactct tttccttctt ccacttcttt ccgttttcag ccgcttccaa
     1621 ccaaaccgat atgaaagccg agcaacaacg atcgattcca cccggctcgg cgaccttccc
     1681 gtcgcaggtg agactcagaa aactagagaa accgctcaac taactcttga tatccgattt
     1741 cattcttttc tcttttcttt ttgttgaact tttccatatt tccagatgcc acggccacca
     1801 ccaagcaccg aacaaggaat cacaacggaa tcggagcttg cgaagaaagc tcaaatcact
     1861 ccgaacgacg ttttagcact tccgggaatc actcaaggta tcttgctccc tttggatttc
     1921 agtttaaaac ataaatttag gattcttatg ctccccatct gcgaacatct ataacatcga
     1981 gttcaccaag ttccaaatcc gtgatctgga cactgagcaa gtgcttttcg agatcgccaa
     2041 accggagaac gatcaggaga atgatgagtc gccacaggag tcggcaagat acgtgcgtta
     2101 tagatttgct ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt
     2161 aggagacatc ccaatccatc atttccgaat gatcgaacgt cacttcttca aagatcgcct
     2221 tctgaagtgt tttgactttg aattcggatt ctgtattccg aattcacgaa acaactgtga
     2281 acatatctat gagttccctc aactctctca acaactcagt gagtcattat tctaaaaagt
     2341 acaattcaaa agactaatct ctttttcagt ggacgacatg atcaacaatc caaacgagac
     2401 tcgttctgac agcttctatt tcgtcgataa caaactcgtc atgcacaaca aagccgacta
     2461 ctcatatgat gcataaatat ttaatacaaa aatgttctgg ataattattc tgtcgaatag
     2521 aaaaaaaact ccaaatgtga ttaaattcca ataattcctg tctagtttgt tccttcccct
     2581 tccccttctc atgttcaatg cattcctaag cttttcagtt ccccccttgt ttctatattt
     2641 tttcgctgtc ctgtcacact cgctaaaaac actaatcaca cggaaatctg ttttcaataa
     2701 aaactccaac tttaactcat tttcaatttc aactgaaaga ttttttcatt agagaatgtc
     2761 tagaactagg cccgggctac gtaatacgac tcacttaagg tgccgctcta gaactagtgg
     2821 atccaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2881 tcgcattata cgagacgtcc aggttgggat acctgaaaca aaacccatcg tacggccaag
     2941 gaagtctcca ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgttgt
     3001 aaaacgacgg ccagtcatgc ataatccgca cgcatctgga ataaggaagt gccattccgc
     3061 ctgacctttc tgcattgttc taaaaactca caggttatct caaaaagata aatttcaaag
     3121 tgagattttt ttaaaaaaat tccggactgt tttttgattt tgatgttttt aatgaatttt
     3181 taaaattctt tattagtgcc tgaaattaac aataatttta aagtttgtca cactgcgaat
     3241 aaattaatct cacatttcgt atgtttagaa agatgcatga aactccgtcg ttcttatgaa
     3301 gttaattaag acatggctaa ttgaaagccc acatggtcat gaaaaagtaa aacttacaac
     3361 tttttactga atattaacca gtctctataa atatcccagc ttagccggaa tagggtcacg
     3421 aaaacatgaa aataaagctt gaacctattg ttatgatttt caatgccacc ttttgcagtt
     3481 taatatacac tattactact tctcagttca agttcacttc tgagacggaa cctttatttt
     3541 ctaactttct ttaatcttac tttttatgag ccgaatgttc tattttgtaa aatgatcaga
     3601 tacaagccca aagctaattt tgccaataag tgttagtaga tcaattgaat tacaatataa
     3661 tgacctgggc ggaatagatt tttgcactga tttttttcca agcttactct atgaatattc
     3721 atagatcatc ttcaaaggta gcgtgtgttg tagttgtgtt ttggcgggtt cgtgttattt
     3781 gactcagctc aagtagatgt tgtagtcttc cctcaatcaa cgcgtgggct tgaccctctt
     3841 taaattgagt ggaaaccagc caatctattt gaaatctacc cccgaaatga gattcgacga
     3901 tgacacgacg tgtgtgttac gtgcgctgag aatttaggaa aaaaaagata atcttctttt
     3961 cgttagctgc gccacaacct gtgcagataa actccgcaca attgaagttc ttactggtcc
     4021 cacccgattt attcaattgt attgtgtctt tttttcttat ttctctacaa ctctaacttt
     4081 cttcaactat tctctacatc agtgcttttt tccaccatga aaaaagaaat gacgtcgttg
     4141 tttcttagga ggggcggagc ctcaactcaa tggtccgttc gaatagatcc cacccctcat
     4201 ataaatagga ctgaaaccaa agaagttttt aaattttcag cttaccgtgt ttctcaattt
     4261 tcaaaatagt cttttttgtg tctgaacggg ctgatcaatt atgttttttt ttgaatgaaa
     4321 aatcttcctc ccaagcctta aaattccttc caaagatcga aagttatgtc tattcaagtt
     4381 acttcttctt agatcaaata ttaaacaaaa aaatacgcca tcattctctc acatttagta
     4441 attcaaaatt tcgatttgtt ttcgaacttt tgaatttccc gcctaggaag tgacatgagt
     4501 gaaatctcat tagataaatg tgccactcac cgatgacact attttcaaat aagggggggg
     4561 ggtgtttcga ataccttgta cacgcgtttc cgcaaaacca cacaccccga gttcctcagc
     4621 ttcttcccct cgacccatga gccacgtcaa gatgtcagcg tgtcactttt cattgcttca
     4681 ccttctacgg taacgagaaa aggcggggaa tccaggatct ggtgtgactt cttccttctt
     4741 cgtatcttcc taaaaatgtt cgtttacttt ttagcgagtg tcatttgatg ggatgtcgtt
     4801 cttgaggaaa gaggctagaa gcttaatact ctcatatttc atattattgg catcaagacg
     4861 tttattattt aactacaata acgattgctt taacaactaa aaataataat ttcagggtta
     4921 gacaaaaata acttcgtata aagtatccta tacgaagtta tatgtccgaa ctcatcaagg
     4981 agaacatgca catgaagctc tacatggagg gaaccgtaga caaccaccac ttcaagtgca
     5041 cctccgaggg agagggaaag ccatacgagg gaacccaaac catgcgtatc aaggtcgtcg
     5101 agggaggacc actcccattc gccttcgaca tcctcgccac ctccttcctc tacgggtcca
     5161 aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagaccttca
     5221 tcaaccacac ccaaggaatc ccagacttct tcaagcaatc cttcccagag ggattcacct
     5281 gggagcgtgt caccacctac gaggacggag gagtcctcac cgccacccaa gacacctccc
     5341 tccaagacgg atgcctcatc tacaacgtca agatccgtgg agtcaacttc acctccaacg
     5401 gaccagtcat gcaaaagaag accctcggat gggagacctt caccgagacc ctctacccag
     5461 ccgacggagg actagaggga cgtaacgaca tggccctcaa gctcgtcgga ggctcccacc
     5521 tcatcgccaa cgccaaggta agtttaaaca gttcggtact aactaaccat acatatttaa
     5581 attttcagac cacctaccgt tccaagaagc cagccaagaa cctcaagatg ccaggagtct
     5641 actacgttga ctaccgtcta gagcgtatca aggaggccaa caacgagacc tacgtcgagc
     5701 aacacgaggt cgccgtcgcc cgttactgcg acctcccatc caagctcgga cacaagctca
     5761 actaagataa atgcaaaatc ctttcaagca ttcccttctt ctctatcact cttctttctt
     5821 tttgtcaaaa aattctctcg ctaatttatt tgctttttta atgttattat tttatgactt
     5881 tttatagtca ctgaaaagtt tgcatctgag tgaagtgaat gctatcaaaa tgtgattctg
     5941 tctgatgtac tttcacaatc tctcttcaat tccattttga agtgctttaa acccgaaagg
     6001 ttgagaaaaa tgcgagcgct caaatatttg tattgtgttc gttgagtgac ccaacaaaaa
     6061 gaggaaactt tattgtgccg ccaagaaaaa agtctcagcc ggcataactt cgtataaagt
     6121 atcctatacg aagttatatg gcccacgccc caggaaccga ccaaatgttc tacgtcggaa
     6181 ccatggacgg atggtacctc gacaccaagc tcaactccgt cgccatcgga gcccactggt
     6241 cctgcttcat cgtcctcacc atcaccacct tctacctcgg atacgagtcc tggacctccc
     6301 gtggaccatc caagcgtacc tccttctacg ccggatacca agaggagcaa aacctcgccc
     6361 tcttcgtcaa cttcttcgcc atgctctcct acttcggaaa gatcgtcgcc gacaccctcg
     6421 gacacaactt cggagacgtc ggaccattca tcatcggatt cggaaactac cgttacgccg
     6481 actacatgct cacctgccca atgctcgtct acgacctcct ctaccaactc cgtgccccat
     6541 accgtgtctc ctgctccgcc atcatcttcg ccatcctcat gtccggagtc ctcgccgagt
     6601 tctacgccga gggagaccca cgtctccgta acggagccta cgcctggtac ggattcggat
     6661 gcttctggtt catcttcgcc tactccatcg tcatgtccat cgtcgccaag caatactccc
     6721 gtctcgccca actcgcccaa gacaccggag ccgagcactc cctccacgtc ctcaagttcg
     6781 ccgtcttcac cttctccatg ctctggatcc tcttcccact cgtctgggcc atctgcccac
     6841 gtggattcgg atggatcgac gacaactgga ccgaggtcgc ccactgcgtc tgcgacatcg
     6901 tcgccaagtc ctgctacgga ttcgccctcg cccgtttccg taaggtaagt ttaaacatat
     6961 atatactaac taaccctgat tatttaaatt ttcagaccta cgacgaggag ctcttccgtc
     7021 tcctcgagca actcggacac gacgaggacg agttccaaaa gctcgagctc gacatgcgtc
     7081 tctcctccaa cggagagcgt ctccgtcgtc tctccgccgc cgccaagtcc cgtatcacct
     7141 ccgagggaga gtacatccca ctcgaccaaa tcgacatcaa cgtctaagct gtctcatcct
     7201 actttcacct agttaactgc ttgtcttaaa atctatgctt ctctttagta tctaaaattt
     7261 tcctagaagc ttacaagtat ataaatggtc tcttctcaat aaaggttgta tatttattca
     7321 tcttattgaa tctgccattt cctcgttttt gcgagtttat ataccttcca attttctttc
     7381 tattgtattt tcaacttcta attttaattc agggaaactg ctgtaccggt agaaaaaatg
     7441 gtcgactcat cacgtagaaa atggaacaaa gctggtcacg ctgtccgtgc catcggacgt
     7501 ctctcctctc cagtcgtctc cgagcgtatg tacccagagg acggagccct caagtccgag
     7561 atcaagaagg gactccgtct caaggacgga ggacactacg ccgccgaggt caagaccacc
     7621 tacaaggcca agaagccagt ccaactccca ggagcctaca tcgtcgacat caagctcgac
     7681 atcgtctccc acaacgagga ctacaccatc gtcgagcaat gcgagcgtgc cgagggacgt
     7741 cactccaccg gaggaatgga cgagctctac aagggaggaa ccggaggttc cctcgtctcc
     7801 aagggagagg aggacaacat ggccatcatc aaggagttca tgcgtttcaa ggtccacatg
     7861 gagggttccg tcaacggaca cgagttcgag atcgagggag agggagaggg acgtccatac
     7921 gaggccttcc aaaccgccaa gctcaaggtc accaagggag gaccactccc attcgcctgg
     7981 gacatcctct ccccacaatt catgtacggc tccaaggcct acatcaagca cccagccgac
     8041 atcccagact acttcaagct ctccttccca gagggattcc gttgggagcg tgtcatgaac
     8101 ttcgaggacg gaggaatcat ccacgtcaac caagactcct ccctccaaga cggagtcttc
     8161 atctacaagg tcaagctccg tggaaccaac ttcccaccag acggaccagt catgcaaaag
     8221 aagaccatgg gatgggaggc cacccgtgac gacctcaccg aggagcaaat cgccgagttc
     8281 aaggaggcct tctccctctt cgacaaggac ggagacggaa ccatcaccac caaggtaagt
     8341 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggagct cggaaccgtc
     8401 ttccgttccc tcggacaaaa cccaaccgag gccgagctcc aagacatgat caacgaggtc
     8461 gacgccgacg gagacggaac cttcgacttc ccagagttcc tcaccatgat ggcccgtaag
     8521 atgaacgaca ccgactccga ggaggagatc cgtgaggcct tccgtgtctt cgacaaggac
     8581 ggaaacggat acatcggagc cgccgagctc cgtcacgtca tgaccgacct cggagagaag
     8641 ctcaccgacg aggaggtcga cgagatgatc cgtgtcgccg acatcgacgg agacggacaa
     8701 gtcaactacg aggagttcgt ccaaatgatg accgccaagt aaggtaccga attcgctagc
     8761 cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat
     8821 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
     8881 ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat
     8941 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     9001 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     9061 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     9121 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     9181 taggcgaacg atgagaattg tcctcgcttg ggccctctga cttctcccgg gcaaaggcta
     9241 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
     9301 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
     9361 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
     9421 tgtgagcaaa aggccagcaa aaggccagga ctagtgagtc gtattataag tgcaagtaag
     9481 atcagtgttt gtttcgatat cagtctgttt cgtaacggtc ttctgtataa ctacaaaaaa
     9541 gaataaaaaa ccgtatcaca actgagaaaa aagacaaaac ttattgaaca gtgttccttt
     9601 caaaaaatta ttttgtaaaa aatcgagttt ttctccaaaa taacgcacgc aatcaaaaac
     9661 aagaaaacta acgtgttctc ctgggcgtgg aactccttat caagattacc attattgtct
     9721 gtcagaattg atatttcaat tatttcatat ctagacaaac ccgggtgtct tgcatttatt
     9781 agtactgtga gtttacatac aaacgaggct tactcagata acatagagtc acctctaatt
     9841 agcaaaaacc catttctaat tagctatttc gaccagttct aaagtcaccg taattagaaa
     9901 tggtttttgt agcttttcta attagaaatg accccgagat aagtgaaata tggcattttc
     9961 tgggttttct agtaagaaat gatcccgcac gtagtgattc cgcgttacaa gccaaattca
    10021 aattaaaaaa gtatattaca caattcaagt tacattattt tcaggaaaca atggcgaaga
    10081 gcaaagcaga gattcaaaag gaggaaagag acaacagata tgaacgaaga cttcttgaaa
    10141 agagaaagag acatgaggtg agtaaccatg ataataaata atcaagtaag ggctatagat
    10201 ttataaattc tggagttaac agtttcgaaa aaaagtagtt ttcatatgtg cgaaggaata
    10261 tcaaaaatag gggaatagcc taaacgagtg agaaaattaa atgtttgtta ttttttcaaa
    10321 aatgtctgca gggccgttat cctttcaaat gcactctatc aaacatagat catatccatc
    10381 atatcttatc cagaatttat atcatatcca agattgagag cgtttcaagg aaaatctcaa
    10441 acaaccatac aattttgtag atcgacaaca aggcctccac ttcttcatcg atggcctctg
    10501 cagcacctgc accaccaagt ttgtattttt catattaata taaagtgagc cacgcatagt
    10561 cgtttttagg atttttgcca gatcgatgtc gcgcaaacag actgtttttt tttctttgaa
    10621 agccgtgcgt gacatgggcc acggattgta acgccgcgct ggatggataa aaatttattc
    10681 agttttttag cctttacaaa atatggttcg actactttct ttatttttac atttttatag
    10741 gtgctcattg agctgagata ttttgggtat aatcggaaaa tcgctgattt tggtcatttt
    10801 tctgcactaa agattacaca aaaagttcag tatttagatt cattgttgaa aattggggat
    10861 gatctacaga tacatatcgc cgtgttcaaa aacacacgtg ctgtgacgtc gtgactgggg
    10921 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    10981 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    11041 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
    11101 ggtgcactct cagtgcaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
    11161 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    11221 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    11281 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    11341 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    11401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    11461 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    11521 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    11581 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    11641 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    11701 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    11761 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    11821 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    11881 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    11941 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    12001 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    12061 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    12121 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    12181 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggtca gatggtaagc
    12241 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    12301 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    12361 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    12421 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    12481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    12541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    12601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    12661 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    12721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    12781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    12841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    12901 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    12961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    13021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    13081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct
    13141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    13201 gtattaccgc ctttgagtga gctgatacca gcggataaca atttcacagg gccctgtcgg
    13261 attatgggct cttcttttat cctctacttt ctttctagaa aatgcaaatg ttgagtcaga
    13321 aattgctgaa tggagtacaa ctgcaatggg tttagcttat gtttctggaa caattactgg
    13381 tggaattgtg attgaaaagt aaggttatat ttcatttcaa taaatgttac cacagtataa
    13441 caaaaacaag ttacattgtt cagagtatgt ctgaatgtat tattattatt gtatttgtgc
    13501 atcagtactg tagttgtact gaagaagggt ttaaaataat ctcaacgttt ttaagacgag
    13561 ttagtgagaa tacggtcgca gttaggatgt ttggacatag ttttattaga taatcgtgaa
    13621 ctaactcact ataaaaaccc gctaactcat tgaataagat agttttaaac gttcaaaatt
    13681 tgaagacaaa attgtatgaa taagtttaca gagttggtcg acgcaagctt ctccttctct
    13741 tcacatttct caataattta gctctaattg cattcgtatt cttcgcaaaa atacgtattc
    13801 tgattgaccc gatgaaatac ggatgcctag ttgcattgat catctacggt tatacttatg
    13861 ggtgaggttt ttcaaatata ttttgattta atcgaataat gaaatttcag aactggagtt
    13921 ggtccaattt cgtggtttat atcctctg
//
