LOCUS       sequence-404776-        6239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VSV-G expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW,
            Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom 
            JD
  TITLE     A pseudovirus system enables deep mutational scanning of the full 
            SARS-CoV-2 spike.
  JOURNAL   Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 
            10.1016/j.cell.2023.02.001. Epub 2023 Feb 13.
  PUBMED    36868218
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.02.001"; journalName: "Cell"; date: 
            "2023-03-16- 16"; volume: "186"; issue: "6"; pages: "1263-1278.e20"
COMMENT     Sequence Label: HDM_VSV_G
FEATURES             Location/Qualifiers
     source          1..6239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          779..1254
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             join(1316..1363,1364..2851)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(3009..3025)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3010..3026)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3119..3513
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3847..3865)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3933..4037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4038..4106,4107..4898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4256..4275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5069..5657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5558..5577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattctg acactatgaa
     1321 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
     1381 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
     1441 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc
     1501 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
     1561 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac
     1621 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
     1681 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
     1741 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
     1801 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
     1861 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
     1921 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
     1981 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
     2041 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
     2101 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
     2161 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
     2221 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
     2281 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
     2341 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
     2401 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
     2461 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
     2521 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
     2581 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
     2641 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
     2701 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
     2761 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
     2821 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcacaacag attcttcatg
     2881 tttggaccaa atcaacttgt gataccatgc tcaaagaggc ctcaattata tttgagtttt
     2941 taatttttat gaaaaaaaaa aaaaaaacgg aattccgcgg gcggccgctc tagaggatcc
     3001 aagcttatcg ataccgtcga cctcgagggc ccagatctaa ttcaccccac cagtgcaggc
     3061 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc
     3121 tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta
     3181 aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta
     3241 ttttcattgc aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg
     3301 gaggtcagtg catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac
     3361 actatatctt aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa
     3421 cagcccctga tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat
     3481 ttggaggtta aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca
     3541 tgaatgtctt ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag
     3601 ttctcttgct tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag
     3661 atggtttctc ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta
     3721 cttgaagaag gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc
     3781 tcccccactc acagtgaccc ggaatccctc gacatggcag tctagatcat tcttgaagac
     3841 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3901 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5641 ggcggagcct atggaaaaac gccagcaacg gagatgcgcc gcgtgcggct gctggagatg
     5701 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     5761 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     5821 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     5881 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     5941 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     6001 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     6061 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     6121 tcggggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     6181 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatg
//
