LOCUS       sequence-404779-       12403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone with BA.1 spike under an inducible promoter and 
            constitutive expressed zsGreen-T2A-PuR genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW,
            Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom 
            JD
  TITLE     A pseudovirus system enables deep mutational scanning of the full 
            SARS-CoV-2 spike.
  JOURNAL   Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 
            10.1016/j.cell.2023.02.001. Epub 2023 Feb 13.
  PUBMED    36868218
REFERENCE   2  (bases 1 to 12403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.02.001"; journalName: "Cell"; date: 
            "2023-03-16- 16"; volume: "186"; issue: "6"; pages: "1263-1278.e20"
COMMENT     Sequence Label: 
            pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR
FEATURES             Location/Qualifiers
     source          1..12403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        200..567
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    244..262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    280..298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    316..334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    352..370
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    388..406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    424..442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     enhancer        4464..4767
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4768..4971
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4922..4942
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4968..4992
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5061..5070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5067..5759
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     CDS             5778..5831
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5832..6431
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5832..5851)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6328..6348
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6439..7027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6492..6512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6910..6921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7101..7734
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7783..7801)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7869..7973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7974..8042,8043..8834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8192..8211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9005..9593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9747..9764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10004..10025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10040..10045,10046..10063,10064..10070)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10078..10094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10083..10105
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10102..10118
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10102..10118
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10168..10187
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10379..11012
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11059..11184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11681..11914
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12099..12143
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
       61 cacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      121 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      181 gtttggacta gtctctctcg agtttactcc ctatcagtga tagagaacgt atgaagagtt
      241 tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac
      301 gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca
      361 gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt
      421 tactccctat cagtgataga gaacgtataa gctttgctta tgtaaaccag ggcgcctata
      481 aaagagtgct gattttttga gtaaacttca attccacaac acttttgtct tataccaact
      541 ttccgtacca cttcctaccc tcgtaaagaa ttcgcggccg cacgcgcagc cgagccacat
      601 cgctcaacgc gtgccaccat gtttgtgttc ctggtgctgc tgccactggt gtccagccag
      661 tgtgtgaacc tgaccaccag gacccaactt cctcctgcct acaccaactc cttcaccagg
      721 ggagtctact accctgacaa ggtgttcagg tcctctgtgc tgcacagcac ccaggacctg
      781 ttcctgccat tcttcagcaa tgtgacctgg ttccatgtga tctctggcac caatggcacc
      841 aagaggtttg acaaccctgt gctgccattc aatgatggag tctactttgc cagcatcgag
      901 aagagcaaca tcatcagggg ctggattttt ggcaccaccc tggacagcaa gacccagtcc
      961 ctgctgattg tgaacaatgc caccaatgtg gtgattaagg tgtgtgagtt ccagttctgt
     1021 aatgacccat tcctggacca caagaacaac aagtcctgga tggagtctga gttcagggtc
     1081 tactcctctg ccaacaactg tacctttgaa tatgtgagcc aaccattcct gatggacttg
     1141 gagggcaagc agggcaactt caagaacctg agggagtttg tgttcaagaa cattgatggc
     1201 tacttcaaga tttacagcaa acacacacca atcatcgtga gggagcccga ggacctgcca
     1261 cagggcttct ctgccttgga accactggtg gacctgccaa ttggcatcaa catcaccagg
     1321 ttccagaccc tgctggctct gcacaggtcc tacctgacac ctggagactc ctcctctggc
     1381 tggacagcag gagcagcagc ctactatgtg ggctacctcc aaccaaggac cttcctgctg
     1441 aaatacaatg agaatggcac catcacagat gctgtggact gtgccctgga cccactgtct
     1501 gagaccaagt gtaccctgaa atccttcaca gtggagaagg gcatctacca gaccagcaac
     1561 ttcagggtcc aaccaacaga gagcattgtg aggtttccaa acatcaccaa cctgtgtcca
     1621 tttgacgagg tgttcaatgc caccaggttt gcctctgtct atgcctggaa caggaagagg
     1681 attagcaact gtgtggctga ctactctgtg ctctacaacc tggccccctt cttcaccttc
     1741 aagtgttatg gagtgagccc aaccaaactg aatgacctgt gtttcaccaa tgtctatgct
     1801 gactcctttg tgattagggg agatgaggtg agacagattg cccctggaca aacaggcaac
     1861 attgctgact acaactacaa actgcctgat gacttcacag gctgtgtgat tgcctggaac
     1921 agcaacaagc tggacagcaa ggtgagcggc aactacaact acctctacag actgttcagg
     1981 aagagcaacc tgaaaccatt tgagagggac atcagcacag agatttacca ggctggcaac
     2041 aagccatgta atggagtggc cggcttcaac tgttactttc cactccgctc ctatagcttc
     2101 agaccaacct acggagtggg ccaccaacca tacagggtgg tggtgctgtc ctttgaactg
     2161 ctccatgccc ctgccacagt gtgtggacca aagaagagca ccaacctggt gaagaacaag
     2221 tgtgtgaact tcaacttcaa tggactgaag ggcacaggag tgctgacaga gagcaacaag
     2281 aagttcctgc cattccaaca gtttggcagg gacattgctg acaccacaga tgctgtgagg
     2341 gacccacaga ccttggagat tctggacatc acaccatgtt cctttggagg agtgtctgtg
     2401 attacacctg gcaccaacac cagcaaccag gtggctgtgc tctaccaggg tgtgaactgt
     2461 actgaggtgc ctgtggctat ccatgctgac caacttacac caacctggag ggtctacagc
     2521 acaggcagca atgtgttcca gaccagggct ggctgtctga ttggagcaga gtacgtgaac
     2581 aactcctatg agtgtgacat cccaattgga gcaggcatct gtgcctccta ccagacccag
     2641 accaagagcc acaggagggc aaggtctgtg gcaagccaga gcatcattgc ctacacaatg
     2701 agtctgggag cagagaactc tgtggcttac agcaacaaca gcattgccat cccaaccaac
     2761 ttcaccatct ctgtgaccac agagattctg cctgtgagta tgaccaagac ctctgtggac
     2821 tgtacaatgt atatctgtgg agacagcaca gagtgtagca acctgctgct ccaatatggc
     2881 tccttctgta cccaacttaa gagggctctg acaggcattg ctgtggaaca ggacaagaac
     2941 acccaggagg tgtttgccca ggtgaagcag atttacaaga cacctccaat caagtacttt
     3001 ggaggcttca acttcagcca gattctgcct gacccaagca agccaagcaa gaggtccttc
     3061 attgaggacc tgctgttcaa caaggtgacc ctggctgatg ctggcttcat caagcaatat
     3121 ggagactgtc tgggagacat tgctgccagg gacctgattt gtgcccagaa gttcaaggga
     3181 ctgacagtgc tgcctccact gctgacagat gagatgattg cccaatacac ctctgccctg
     3241 ctggctggca ccatcacctc tggctggacc tttggagcag gagcagccct ccaaatccca
     3301 tttgctatgc agatggctta caggttcaat ggcattggag tgacccagaa tgtgctctat
     3361 gagaaccaga aactgattgc caaccagttc aactctgcca ttggcaagat tcaggactcc
     3421 ctgtccagca cagcctctgc cctgggcaaa ctccaagatg tggtgaacca caatgcccag
     3481 gctctgaaca ccctggtgaa gcaactttcc agcaagtttg gagccatctc ctctgtgctg
     3541 aatgacatct tcagcagact ggacaaggtg gaggctgagg tccagattga cagactgatt
     3601 acaggcagac tccaatccct ccaaacctat gtgacccaac aacttatcag ggctgctgag
     3661 attagggcat ctgccaacct ggctgccacc aagatgagtg agtgtgtgct gggacaaagc
     3721 aagagggtgg acttctgtgg caagggctac cacctgatga gttttccaca gtctgcccct
     3781 catggagtgg tgttcctgca tgtgacctat gtgcctgccc aggagaagaa cttcaccaca
     3841 gcccctgcca tctgccatga tggcaaggct cactttccaa gggagggagt gtttgtgagc
     3901 aatggcaccc actggtttgt gacccagagg aacttctatg aaccacagat tatcaccaca
     3961 gacaacacct ttgtgtctgg caactgtgat gtggtgattg gcattgtgaa caacacagtc
     4021 tatgacccac tccaacctga actggactcc ttcaaggagg aactggacaa atacttcaag
     4081 aaccacacca gccctgatgt ggacctggga gacatctctg gcatcaatgc ctctgtggtg
     4141 aacatccaga aggagattga cagactgaat gaggtggcta agaacctgaa tgagtccctg
     4201 attgacctcc aagaactggg caaatatgaa caatacatca agtggccatg gtacatctgg
     4261 ctgggcttca ttgctggact gattgcaatt gtgatggtga caataatgct gtgttgtatg
     4321 acctcctgtt gttcctgtct gaaaggctgt tgttcctgtg gctcctgata atgaggcgcg
     4381 cctagttgga tcacactctt tccctacacg acgctcttcc gatcttctag attcgagaga
     4441 gaaatgttcc tagtggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4501 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4561 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4621 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4681 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4741 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4801 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4861 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4921 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     4981 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     5041 ctatagtgag tcgtattacg gccgccatgg cccagtccaa gcacggcctg accaaggaga
     5101 tgaccatgaa gtaccgcatg gagggctgcg tggacggcca caagttcgtg atcaccggcg
     5161 agggcatcgg ctaccccttc aagggcaagc aggccatcaa cctgtgcgtg gtggagggcg
     5221 gccccttgcc cttcgccgag gacatcttgt ccgccgcctt catgtacggc aaccgcgtgt
     5281 tcaccgagta cccccaggac atcgtcgact acttcaagaa ctcctgcccc gccggctaca
     5341 cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg catctgcaac gccgacatca
     5401 ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa gttctacggc gtgaacttcc
     5461 ccgccgacgg ccccgtgatg aagaagatga ccgacaactg ggagccctcc tgcgagaaga
     5521 tcatccccgt gcccaagcag ggcatcttga agggcgacgt gagcatgtac ctgctgctga
     5581 aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta caaggccaag tccgtgcccc
     5641 gcaagatgcc cgactggcac ttcatccagc acaagctgac ccgcgaggac cgcagcgacg
     5701 ccaagaacca gaagtggcac ctgaccgagc acgccatcgc ctccggctcc gccttgcccg
     5761 ctggatccgg ctccggcgag ggcaggggaa gtcttctaac atgcggggac gtggaggaaa
     5821 atcccggccc aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5881 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5941 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6001 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6061 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6121 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6181 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6241 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6301 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6361 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgtaagc
     6421 ccggtgcctg aggatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6841 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7021 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7081 tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac aagatatcct
     7141 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     7201 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     7261 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     7321 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     7381 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     7441 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     7501 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     7561 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7621 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7681 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtcccg
     7741 ggcgattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     7801 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7861 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7921 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7981 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8041 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8101 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8161 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     8221 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8281 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8341 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8401 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8461 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8521 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8581 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8641 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8701 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8761 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8821 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8881 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8941 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9061 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9781 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
     9841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9901 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     9961 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10021 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    10081 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
    10141 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10201 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    10261 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    10321 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
    10381 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
    10441 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
    10501 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
    10561 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    10621 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
    10681 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
    10741 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
    10801 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
    10861 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10921 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10981 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    11041 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    11101 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    11161 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    11221 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    11281 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    11341 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    11401 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    11461 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    11521 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    11581 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    11641 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    11701 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    11761 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    11821 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    11881 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    11941 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    12001 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    12061 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    12121 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    12181 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    12241 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
    12301 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
    12361 gagacagatc cattcgatta gtgaacggat ctcgacggta tcg
//
