LOCUS       sequence-404782-       12388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone with Delta spike under an inducible promoter and
            constitutive expressed zsGreen-T2A-PuR genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW,
            Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom 
            JD
  TITLE     A pseudovirus system enables deep mutational scanning of the full 
            SARS-CoV-2 spike.
  JOURNAL   Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 
            10.1016/j.cell.2023.02.001. Epub 2023 Feb 13.
  PUBMED    36868218
REFERENCE   2  (bases 1 to 12388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.02.001"; journalName: "Cell"; date: 
            "2023-03-16- 16"; volume: "186"; issue: "6"; pages: "1263-1278.e20"
COMMENT     Sequence Label: 
            pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR
FEATURES             Location/Qualifiers
     source          1..12388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        200..567
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    244..262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    280..298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    316..334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    352..370
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    388..406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    424..442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(1925..1942)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     enhancer        4467..4770
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4771..4974
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4925..4945
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4971..4995
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5046..5055
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5052..5744
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     CDS             5763..5816
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5817..6416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5817..5836)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6313..6333
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6424..7012
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6477..6497)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6895..6906)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7086..7719
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7768..7786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7854..7958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7959..8027,8028..8819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8177..8196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8990..9578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9732..9749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9989..10010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10025..10030,10031..10048,10049..10055)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10063..10079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10068..10090
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10087..10103
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10087..10103
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10153..10172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10364..10997
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11044..11169
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11666..11899
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12084..12128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
       61 cacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      121 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      181 gtttggacta gtctctctcg agtttactcc ctatcagtga tagagaacgt atgaagagtt
      241 tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac
      301 gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca
      361 gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt
      421 tactccctat cagtgataga gaacgtataa gctttgctta tgtaaaccag ggcgcctata
      481 aaagagtgct gattttttga gtaaacttca attccacaac acttttgtct tataccaact
      541 ttccgtacca cttcctaccc tcgtaaagaa ttcgcggccg cacgcgcagc cgagccacat
      601 cgctcaacgc gtgccaccat gtttgtgttc ctggtgctgc tgccactggt gtccagccag
      661 tgtgtgaacc tgagaaccag gacccaactt cctcctgcct acaccaactc cttcaccagg
      721 ggagtctact accctgacaa ggtgttcagg tcctctgtgc tgcacagcac ccaggacctg
      781 ttcctgccat tcttcagcaa tgtgacctgg ttccatgcca tccatgtgtc tggcaccaat
      841 ggcaccaaga ggtttgacaa ccctgtgctg ccattcaatg atggagtcta ctttgccagc
      901 acagagaaga gcaacatcat caggggctgg atttttggca ccaccctgga cagcaagacc
      961 cagtccctgc tgattgtgaa caatgccacc aatgtggtga ttaaggtgtg tgagttccag
     1021 ttctgtaatg acccattcct ggacgtctac taccacaaga acaacaagtc ctggatggag
     1081 tctggagtct actcctctgc caacaactgt acctttgaat atgtgagcca accattcctg
     1141 atggacttgg agggcaagca gggcaacttc aagaacctga gggagtttgt gttcaagaac
     1201 attgatggct acttcaagat ttacagcaaa cacacaccaa tcaacctggt gagggacctg
     1261 ccacagggct tctctgcctt ggaaccactg gtggacctgc caattggcat caacatcacc
     1321 aggttccaga ccctgctggc tctgcacagg tcctacctga cacctggaga ctcctcctct
     1381 ggctggacag caggagcagc agcctactat gtgggctacc tccaaccaag gaccttcctg
     1441 ctgaaataca atgagaatgg caccatcaca gatgctgtgg actgtgccct ggacccactg
     1501 tctgagacca agtgtaccct gaaatccttc acagtggaga agggcatcta ccagaccagc
     1561 aacttcaggg tccaaccaac agagagcatt gtgaggtttc caaacatcac caacctgtgt
     1621 ccatttggag aggtgttcaa tgccaccagg tttgcctctg tctatgcctg gaacaggaag
     1681 aggattagca actgtgtggc tgactactct gtgctctaca actctgcctc cttcagcacc
     1741 ttcaagtgtt atggagtgag cccaaccaaa ctgaatgacc tgtgtttcac caatgtctat
     1801 gctgactcct ttgtgattag gggagatgag gtgagacaga ttgcccctgg acaaacaggc
     1861 aagattgctg actacaacta caaactgcct gatgacttca caggctgtgt gattgcctgg
     1921 aacagcaaca acctggacag caaggtggga ggcaactaca actacagata cagactgttc
     1981 aggaagagca acctgaaacc atttgagagg gacatcagca cagagattta ccaggctggc
     2041 agcaagccat gtaatggagt ggagggcttc aactgttact ttccactcca atcctatggc
     2101 ttccaaccaa ccaatggagt gggctaccaa ccatacaggg tggtggtgct gtcctttgaa
     2161 ctgctccatg cccctgccac agtgtgtgga ccaaagaaga gcaccaacct ggtgaagaac
     2221 aagtgtgtga acttcaactt caatggactg acaggcacag gagtgctgac agagagcaac
     2281 aagaagttcc tgccattcca acagtttggc agggacattg ctgacaccac agatgctgtg
     2341 agggacccac agaccttgga gattctggac atcacaccat gttcctttgg aggagtgtct
     2401 gtgattacac ctggcaccaa caccagcaac caggtggctg tgctctacca gggtgtgaac
     2461 tgtactgagg tgcctgtggc tatccatgct gaccaactta caccaacctg gagggtctac
     2521 agcacaggca gcaatgtgtt ccagaccagg gctggctgtc tgattggagc agagcatgtg
     2581 aacaactcct atgagtgtga catcccaatt ggagcaggca tctgtgcctc ctaccagacc
     2641 cagaccaaca gcagaaggag ggcaaggtct gtggcaagcc agagcatcat tgcctacaca
     2701 atgagtctgg gagcagagaa ctctgtggct tacagcaaca acagcattgc catcccaacc
     2761 aacttcacca tctctgtgac cacagagatt ctgcctgtga gtatgaccaa gacctctgtg
     2821 gactgtacaa tgtatatctg tggagacagc acagagtgta gcaacctgct gctccaatat
     2881 ggctccttct gtacccaact taacagggct ctgacaggca ttgctgtgga acaggacaag
     2941 aacacccagg aggtgtttgc ccaggtgaag cagatttaca agacacctcc aatcaaggac
     3001 tttggaggct tcaacttcag ccagattctg cctgacccaa gcaagccaag caagaggtcc
     3061 ttcattgagg acctgctgtt caacaaggtg accctggctg atgctggctt catcaagcaa
     3121 tatggagact gtctgggaga cattgctgcc agggacctga tttgtgccca gaagttcaat
     3181 ggactgacag tgctgcctcc actgctgaca gatgagatga ttgcccaata cacctctgcc
     3241 ctgctggctg gcaccatcac ctctggctgg acctttggag caggagcagc cctccaaatc
     3301 ccatttgcta tgcagatggc ttacaggttc aatggcattg gagtgaccca gaatgtgctc
     3361 tatgagaacc agaaactgat tgccaaccag ttcaactctg ccattggcaa gattcaggac
     3421 tccctgtcca gcacagcctc tgccctgggc aaactccaaa acgtggtgaa ccagaatgcc
     3481 caggctctga acaccctggt gaagcaactt tccagcaact ttggagccat ctcctctgtg
     3541 ctgaatgaca tcctgagcag actggacaag gtggaggctg aggtccagat tgacagactg
     3601 attacaggca gactccaatc cctccaaacc tatgtgaccc aacaacttat cagggctgct
     3661 gagattaggg catctgccaa cctggctgcc accaagatga gtgagtgtgt gctgggacaa
     3721 agcaagaggg tggacttctg tggcaagggc taccacctga tgagttttcc acagtctgcc
     3781 cctcatggag tggtgttcct gcatgtgacc tatgtgcctg cccaggagaa gaacttcacc
     3841 acagcccctg ccatctgcca tgatggcaag gctcactttc caagggaggg agtgtttgtg
     3901 agcaatggca cccactggtt tgtgacccag aggaacttct atgaaccaca gattatcacc
     3961 acagacaaca cctttgtgtc tggcaactgt gatgtggtga ttggcattgt gaacaacaca
     4021 gtctatgacc cactccaacc tgaactggac tccttcaagg aggaactgga caaatacttc
     4081 aagaaccaca ccagccctga tgtggacctg ggagacatct ctggcatcaa tgcctctgtg
     4141 gtgaacatcc agaaggagat tgacagactg aatgaggtgg ctaagaacct gaatgagtcc
     4201 ctgattgacc tccaagaact gggcaaatat gaacaataca tcaagtggcc atggtacatc
     4261 tggctgggct tcattgctgg actgattgcc attgtgatgg tgaccataat gctgtgttgt
     4321 atgacctcct gttgttcctg tctgaaaggc tgttgttcct gtggctcctg ataatgaggc
     4381 gcgcctagtt ggatcacact ctttccctac acgacgctct tccgatcttc tagattcgag
     4441 agagaaatgt tcctagtgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4501 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4561 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4621 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4681 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4741 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4801 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4861 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4921 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     4981 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     5041 cggcggccgc catggcccag tccaagcacg gcctgaccaa ggagatgacc atgaagtacc
     5101 gcatggaggg ctgcgtggac ggccacaagt tcgtgatcac cggcgagggc atcggctacc
     5161 ccttcaaggg caagcaggcc atcaacctgt gcgtggtgga gggcggcccc ttgcccttcg
     5221 ccgaggacat cttgtccgcc gccttcatgt acggcaaccg cgtgttcacc gagtaccccc
     5281 aggacatcgt cgactacttc aagaactcct gccccgccgg ctacacctgg gaccgctcct
     5341 tcctgttcga ggacggcgcc gtgtgcatct gcaacgccga catcaccgtg agcgtggagg
     5401 agaactgcat gtaccacgag tccaagttct acggcgtgaa cttccccgcc gacggccccg
     5461 tgatgaagaa gatgaccgac aactgggagc cctcctgcga gaagatcatc cccgtgccca
     5521 agcagggcat cttgaagggc gacgtgagca tgtacctgct gctgaaggac ggtggccgct
     5581 tgcgctgcca gttcgacacc gtgtacaagg ccaagtccgt gccccgcaag atgcccgact
     5641 ggcacttcat ccagcacaag ctgacccgcg aggaccgcag cgacgccaag aaccagaagt
     5701 ggcacctgac cgagcacgcc atcgcctccg gctccgcctt gcccgctgga tccggctccg
     5761 gcgagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc ggcccaatga
     5821 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     5881 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     5941 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6001 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6061 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6121 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6181 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6241 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6301 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6361 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg taagcccggt gcctgaggat
     6421 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6481 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6541 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6601 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6661 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6721 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6781 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6841 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6901 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6961 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7021 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7081 gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     7141 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7201 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7261 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7321 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7381 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     7441 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7501 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7561 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7621 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7681 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tcccgggcga ttaaggaaag
     7741 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7801 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     8221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9781 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
     9841 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9901 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
     9961 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    10021 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10081 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
    10141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10201 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10261 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10321 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    10381 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10441 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    10501 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    10561 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    10621 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    10681 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    10741 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    10801 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    10861 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    10921 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    10981 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    11041 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    11101 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    11161 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    11221 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    11281 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    11341 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    11401 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    11461 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
    11521 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    11581 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    11641 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    11701 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    11761 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    11821 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    11881 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    11941 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    12001 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    12061 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    12121 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    12181 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    12241 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    12301 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
    12361 gattagtgaa cggatctcga cggtatcg
//
