LOCUS       sequence-404786-       11634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA editing in plants. Plasmid contains 
            35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G
  TITLE     Proximity editing to identify RNAs in phase-separated RNA binding 
            protein condensates.
  JOURNAL   Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9.
  PUBMED    34426573
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-08-24"; volume: "7(1):72. doi"; pages: " 
            10.1038/s41421-021-00288-9"
COMMENT     Sequence Label: pZG71
FEATURES             Location/Qualifiers
     source          1..11634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2088..2729
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(2737..2754)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2737..2753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2746..2768)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2956..2980
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4281..4910
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5339..6412
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6478..6672
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6908..6930)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7016..7156
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7071..7090
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7171..7188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7342..7930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7422..7441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8017..8811)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9236..9260
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9338..9512
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9553..10578)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10645..11321)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11512..11533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11548..11553,11554..11571,11572..11578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11586..11602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11591..11613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11610..11626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11610..11626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc actagtacca tgtactctcc tatggtcgta ccgcaaaaaa
      901 atgtgcctct ccctatagac gataaatcta gctccatgga gcttccgcag atccacgcag
      961 acactatcgg tcgattggtg ctggaaaagt tcatggaggt tattaaaggt caggaggctt
     1021 atagccgtcg aaaagttttg gctggaatcg taatgaccga aaacatgaac ttctgtgagg
     1081 caaaagtcat ttcagtgtcc acgggcacca agtgtgtgtc cggcgagcat atgtccgtaa
     1141 acggtgcggt actcaatgac agccacgccg agatagtgtc acgtcgttgt ctcctcaagt
     1201 atctctatgc ccaactggat cttcaatgca accaggcgac cgcttatcag agcatatttg
     1261 tgcgtaatac tgacggtcag tatccctata aactgaagtc tggggtccat tttcatctct
     1321 atataaacac ggcgccctgc ggcgatgccc gtattttttc acctcacgag aatgacacgg
     1381 gagtagataa acatcccaac cgaaaagcaa gagggcagct ccgaaccaaa atcgaatccg
     1441 gccagggcac aatcccagtg aaatctagcg acggcattca aacttgggac ggcgtactac
     1501 aaggccagcg tttgcttacg atgtcctgct ccgataaaat tgctaggtgg aacatcgtcg
     1561 gtatccaggg ctccttgctc tcttcaatta tagagcccgt ctatctgcat tcaattgtct
     1621 tagggtcact cttgcatcct gaacatatgt accgagccgt ttgtgggaga atagaaaaga
     1681 gtatccaggg tctaccccca ccttaccatt tgaacaaacc cagactggca ttggtcacat
     1741 ccgcagaacc acgtaaccaa gccaaggccc cgaattttgg aataaactgg actataggtg
     1801 acacggaact agaggttgtg aactctctca caggcagaac cataggaggg caagtcagcc
     1861 gtataacgaa gcaggctttt ttcgtaaagt atggtttctt gatggctaat ttgcccggaa
     1921 tcctcgtccg aaaggtaacg accgattacg gtcagacaaa agcgaatgta aaggattacc
     1981 agatcgcgaa gttggaattg ttctcagcct tcaagaggga ggatcttgga tcatggctaa
     2041 agaaaccaat agagcaggac gagtttggat tggctgaatg actgcagagc tttcgttcgt
     2101 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc gcacacacca
     2161 gaatcctact gagtttgagt attatggcat tgggaaaact gtttttcttg taccatttgt
     2221 tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa
     2281 tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat
     2341 ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt
     2401 ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag
     2461 taaaacactt gtagttgtac cattatgctt attcactagg caacaaatat attttcagac
     2521 ctagaaaagc tgcaaatgtt actgaataca agtatgtcct cttgtgtttt agacatttat
     2581 gaactttcct ttatgtaatt ttccagaatc cttgtcagat tctaatcatt gctttataat
     2641 tatagttata ctcatggatt tgtagttgag tatgaaaata ttttttaatg cattttatga
     2701 cttgccaatt gattgacaac atgcatcaag cttggcactg gccgtcgttt tacaacgtcg
     2761 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2821 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2881 gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt
     2941 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     3001 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     3061 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     3121 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     3181 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     3241 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     3301 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     3361 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     3421 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     3481 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     3541 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     3601 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     3661 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     3721 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3781 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3841 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3901 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3961 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     4021 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     4081 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     4141 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     4201 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     4261 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     4321 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     4381 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     4441 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     4501 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     4561 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     4621 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     4681 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     4741 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4801 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4861 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4921 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4981 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     5041 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     5101 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     5161 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     5221 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     5281 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     5341 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     5401 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     5461 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     5521 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     5581 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     5641 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     5701 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     5761 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     5821 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     5881 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     5941 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     6001 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     6061 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     6121 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     6181 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     6241 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     6301 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     6361 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     6421 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
     6481 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     6541 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     6601 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     6661 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     6721 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     6781 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     6841 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     6901 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6961 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     7021 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     7081 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7141 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7741 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7981 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat
     8041 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     8101 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
     8161 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
     8221 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
     8281 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
     8341 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
     8401 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
     8461 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
     8521 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
     8581 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
     8641 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
     8701 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
     8761 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
     8821 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     8881 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     8941 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
     9001 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
     9061 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
     9121 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
     9181 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
     9241 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
     9301 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
     9361 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
     9421 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
     9481 ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga cagatcccgg
     9541 tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     9601 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     9661 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     9721 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     9781 catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     9841 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     9901 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     9961 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
    10021 ctcggcccaa agcatcagat catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
    10081 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
    10141 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
    10201 atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc
    10261 ggtttcaggc aggtcttgca acgtgacacc ctgtgaacgg cgggagatgc aataggtcag
    10321 gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
    10381 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
    10441 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
    10501 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
    10561 gagttcaggc tttttcatat ctcattgccc cccggatctg cgaaagctcg agagagatag
    10621 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta
    10681 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    10741 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    10801 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    10861 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    10921 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    10981 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
    11041 gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg
    11101 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
    11161 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
    11221 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
    11281 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
    11341 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa
    11401 gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11461 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11521 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    11581 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttac
//
