LOCUS       sequence-404792-       11675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo 
            recombineering and Bxb-1 for pInt incorporation. Has sacB for 
            counter selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Saunders SH, Ahmed AM
  TITLE     ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at 
            high throughput and high efficiency
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pHelper_Ec1_V1_ampR
FEATURES             Location/Qualifiers
     source          1..11675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      850..877
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(900..1691,1692..1760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1523..1542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1761..1865)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1933..1951
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(2117..2995)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(3216..4181)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        5081..5161
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      7985..8079
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     8082..8101
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             8186..8194
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8197..9699
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDVRIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDL*EYEQHLRLGSVVERLHTGMS"
     terminator      9734..9780
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             join(10219..10305,10306..11640)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tttttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccat gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctaga actagttgat
      841 tagctaagca gaaggccatc ctgacggatg gccttttatg agtaaacttg gtctgacagt
      901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1201 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1501 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1861 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     1921 ttaacctata aaaataggcg tatcacgagg ccctttcgtg tctcatgagc ggatacatat
     1981 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgg
     2041 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
     2101 cttataatac agttttttat gacaacttga cggctacatc attcactttt tcttcacaac
     2161 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaagtaga
     2221 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
     2281 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
     2341 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
     2401 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
     2461 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
     2521 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
     2581 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
     2641 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
     2701 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
     2761 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
     2821 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
     2881 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
     2941 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
     3001 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
     3061 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
     3121 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
     3181 acggcagaaa agtccacatt gattatttgc acggctcaag ccacttcctt tttgcattga
     3241 cgcagggtgt cggaaggcaa ctcgccgaac gcgctcctat agttttcagc gaagcgtccc
     3301 aaatgtaaga agccgtagtc tagggctatc tcagttatac tacgcacatt ggcactggga
     3361 tcgttcaagc aggcgcggat gctttcgagc ttgcggttgc ggatgtagtt cttcggcgtg
     3421 gtgccggcgt gcttctcgaa caaattgtag agcgagcgtg gactcatcat cgccagctcc
     3481 gctaaccgct caaggctgat attccgtttg agattctcct caatgaattg aacgactcgc
     3541 tcgaaagacg ggttaccttt gctgaaaatt tcacggctga cattgctgcc cagcatttcg
     3601 agcagcttgg aagcgatgat ccccgcatag tgctcttgga cccgaggcat cgactttgta
     3661 tgttccgctt cgtcacaaac taacccgagt agattgataa agccatcgag ttgctggaga
     3721 ttgtgtcgcg cggcgaaacg gataccctcc ctcggcttgt gccaattgtt gtcactgcac
     3781 gcccgatcaa ggaccactga gggcaattta acgataaatt tctcgcaatc ttctgaatag
     3841 gtcaggtcgg cttggtcatc cggattgagc agcaatagtt cgcccggcgc aaaatagtgc
     3901 tcctggccat ggccacgcca caggcaatgg cctttgagta ttatttgcag atgataacag
     3961 gtctctaatc caggcgagat taccctcacg ctaccgccgt agctgattcg acacagatcg
     4021 aggcatccga agattctgtg gtgcagcctg cctgccgggc gcccgccctt gggcaggcga
     4081 atagagtgcg taccgacata ctggttaaca taatcggaga ctgcataggg ctcggcgtgg
     4141 acgaagatct gacttttctc gttcaataag caaaaatcca tagttcacgg ttctcttatt
     4201 ttaatgtggg ctgcttggtg tgatgtagaa aggcgccaag tcgatgaaaa tgcatctcga
     4261 cgtgatgcgt atacgggtta cccccattgc cacgttgcgc catccttttt gcaatcagtg
     4321 accacttttc caagcaaaaa taacgccaag cagaacgaag acgttctttt taagaagcga
     4381 gaacaccaga agttcgtgct gtcggggcat ggggcgacga attggcggat aaaggggatc
     4441 tgctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     4501 cctttattca cattcttgcc cgcctgatga atgctcatcc gtaattacgt atggcaatga
     4561 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     4621 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     4681 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     4741 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     4801 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgcat gggaattagc
     4861 ttgatctgac caacgaccgg tagcggagct atccaacggc ggtataccag gaaaacacac
     4921 agcaggtaca tcagaacagt accatgactg aagaacaaat agttttttcc tgatccataa
     4981 agcagaacgg cctgctccat gacaaatctg gctccccaac taatgcccca tgcagccagc
     5041 ataaccagca taaagtgcag tgtccggttt gatagggata agtccagcct tgcaagaagc
     5101 ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc taccccttag gctttatgca
     5161 acagaaacaa taataatgga gtcatgacca tgcctaggcc gcgggccgcg cgaattcaga
     5221 aggagaatat accatgaacc aaatcgtgaa gttcactgac gactctggcc tggcggttca
     5281 agttactcca gacgatgttc gccgttatat ctgtgagaac gctactgaaa aagaggtggg
     5341 cctctttctg caactctgtc agactcaacg tctgaatccg tttgtgaaag acgcttacct
     5401 ggtgaaatac ggcggtgctc cagcttctat gattacttcc tatcaagttt ttaaccgtcg
     5461 cgcgtgtcgt gatgctaact atgatggtat caaatctggt gtggttgttc tgcgtgacgg
     5521 tgatgttgtg cataaacgtg gtgctgcgtg ctacaaaaag gcgggtgagg agctcatcgg
     5581 tggttgggcg gaagttcgct ttaaggatgg ccgcgagact gcgtatgctg aggtggcgct
     5641 cgacgactat tccaccggca aatctaattg ggcgaaaatg ccgggtgtta tgatcgaaaa
     5701 atgcgcgaag gctgctgctt ggcgcctcgc gttcccggac acttttcagg gcatgtacgc
     5761 tgcggaggaa atggatcaag cgcaacagcc agaacaggtg cgcgctcagg cggagcaacc
     5821 agtggatctc cagccaatcc gcgaactctt caagccatat tgcgaacact tcggcatcac
     5881 tccggctgag ggtatgactg ctgtttgtgg tgcggtgggc gctgaaggca tgcactctat
     5941 gaccgagcag caagctcgcc gtgctcgcgc ttggatggag gaagaaatgg ctgcgccagc
     6001 tgtggaagcg gagtatgagg ttgttgacga gggcgaggtg ttttaaggat ccaggagaat
     6061 acacatgcca attcaggtct taccgccaca actggcgaac cagattgccg caggtgaggt
     6121 ggtcgagcga cctgcgtcgg tagtcaaaga actagtgaaa aacagcctcg atgcaggtgc
     6181 gacgcgtatc gatattgata tcgaacgcgg tggggcgaaa cttatccgca ttcgtgataa
     6241 cggctgcggt atcaaaaaag atgagctggc gctggcgctg gctcgtcatg ccaccagtaa
     6301 aatcgcctct ctggacgatc tcgaagccat tatcagcctg ggctttcgcg gtgaggcgct
     6361 ggcgagtatc agttcggttt cccgcctgac gctcacttca cgcaccgcag aacagcagga
     6421 agcctggcag gcctatgccg aagggcgcga tatgaacgtg acggtaaaac cggcggcgca
     6481 tcctgtgggg acgacgctgg aggtgctgga tctgttctac aacaccccgg cgcggcgcaa
     6541 attcctgcgc accgagaaaa ccgaatttaa ccacattgat gagatcatcc gccgcattgc
     6601 gctggcgcgt ttcgacgtca cgatcaacct gtcgcataac ggtaaaattg tgcgtcagta
     6661 ccgcgcagtg ccggaaggcg ggcaaaaaga acggcgctta ggcgcgattt gcggcaccgc
     6721 ttttcttgaa caagcgctgg cgattgaatg gcaacacggc gatctcacgc tacgcggctg
     6781 ggtggccgat ccaaatcaca ccacgcccgc actggcagaa attcagtatt gctacgtgaa
     6841 cggtcgcatg atgcgcgatc gcctgatcaa tcacgcgatc cgccaggcct gcgaagacaa
     6901 actgggggcc gatcagcaac cggcatttgt gttgtatctg gagatcgacc cacatcaggt
     6961 ggacgtcaac gtgcaccccg ccaaacacga agtgcgtttc catcagtcgc gtctggtgca
     7021 tgattttatc tatcagggcg tgctgagcgt gctacaacag caactggaaa cgccgctacc
     7081 gctggacgat gaaccccaac ctgcaccgcg ttccattccg gaaaaccgcg tggcggcggg
     7141 gcgcaatcac tttgcagaac cggcagctcg tgagccggta gctccgcgct acactcctgc
     7201 gccagcatca ggcagtcgtc cggctgcccc ctggccgaat gcgcagccag gctaccagaa
     7261 acagcaaggt gaagtgtatc gccagctttt gcaaacgccc gcgccgatgc aaaaattaaa
     7321 agcgccggaa ccgcaggaac ctgcacttgc ggcgaacagt cagagttttg gtcgggtact
     7381 gactatcgtc cattccgact gtgcgttgct ggagcgcgac ggcaacattt cacttttatc
     7441 cttgccagtg gcagaacgtt ggctgcgtca ggcacaattg acgccgggtg aagcgcccgt
     7501 ttgcgcccag ccgctgctga ttccgttgcg gctaaaagtt tctgccgaag aaaaatcggc
     7561 attagaaaaa gcgcagtctg ccctggcgga attgggtatt gatttccagt cagatgcaca
     7621 gcatgtgacc atcagggcag tgcctttacc cttacgccaa caaaatttac aaatcttgat
     7681 tcctgaactg ataggctacc tggcgaagca gtccgtattc gaacctggca atattgcgca
     7741 gtggattgca cgaaatctga tgagcgaaca tgcgcagtgg tcaatggcac aggccataac
     7801 cctgctggcg gacgtggaac ggttatgtcc gcaacttgtg aaaacgccgc cgggtggtct
     7861 gttacaatct gttgatttac atccggcgat aaaagccctg aaagatgagt gatctagagt
     7921 cgacctgcag gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt
     7981 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     8041 acgctcggtt gccgccgggc gttttttatt ggtgagaatc tatgccatag catttttatc
     8101 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     8161 cccgtttttt tgggaattcg agctctaagg aggttaatga gagccctggt agtcatccgc
     8221 ctgtcccgcg tcaccgatgc tacgacttca ccggagcgtc agctggagtc ttgccagcag
     8281 ctctgcgccc agcgcggctg ggacgtcgtc ggggtagcgg aggatctgga cgtctccggg
     8341 gcggtcgatc cgttcgaccg gaagcgcaga ccgaacctgg cccggtggct agcgttcgag
     8401 gagcaaccgt tcgacgtgat cgtggcgtac cgggtagacc ggttgacccg atcgatccgg
     8461 catctgcagc agctggtcca ctgggccgag gaccacaaga agctggtcgt ctccgcgacc
     8521 gaagcgcact tcgatacgac gacgccgttt gcggcggtcg tcatcgcgct tatgggaacg
     8581 gtggcgcaga tggaattaga agcgatcaaa gagcggaacc gttcggctgc gcatttcaat
     8641 atccgcgccg ggaaataccg aggatccctg ccgccgtggg gatacctgcc tacgcgcgtg
     8701 gacggggagt ggcggctggt gccggaccct gtgcagcgag agcgcatcct cgaggtgtat
     8761 caccgcgtcg tcgacaacca cgagccgctg cacctggtgg cccacgacct gaaccggcgt
     8821 ggtgtcctgt cgccgaagga ctacttcgcg cagctgcaag gccgcgagcc gcagggccgg
     8881 gagtggtcgg ctaccgcgct gaagcgatcg atgatctccg aggcgatgct cgggtacgcg
     8941 actctgaacg gtaagaccgt ccgagacgac gacggagccc cgctggtgcg ggctgagccg
     9001 atcctgaccc gtgagcagct ggaggcgctg cgcgccgagc tcgtgaagac ctcccgggcg
     9061 aagcccgcgg tgtctacccc gtcgctgctg ctgcgggtgt tgttctgcgc ggtgtgcggg
     9121 gagcccgcgt acaagttcgc cgggggagga cgtaagcacc cgcgctaccg ctgccgctcg
     9181 atggggttcc cgaagcactg cgggaacggc acggtggcga tggccgagtg ggacgcgttc
     9241 tgcgaggagc aggtgctgga tctgctcggg gacgcggagc gtctggagaa agtctgggta
     9301 gccggctcgg actccgcggt cgaactcgcg gaggtgaacg cggagctggt ggacctgacg
     9361 tcgctgatcg gctccccggc ctaccgggcc ggctctccgc agcgagaagc actggatgtc
     9421 cgtattgcgg cgctggccgc gcggcaagag gagctggagg gtctagaggc tcgcccgtct
     9481 ggctgggagt ggcgcgagac cgggcagcgg ttcggggact ggtggcggga gcaggacacc
     9541 gcggcaaaga acacctggct tcggtcgatg aacgttcggc tgacgttcga cgtccgcggc
     9601 gggctgactc gcacgatcga cttcggggat ctgtaggagt acgagcagca tctcaggctc
     9661 ggcagcgtgg tcgaacggct acacaccggg atgtcgtagg ttaacaacta gcgtacgatc
     9721 gactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     9781 gccatcatgg ccgcgggacg tcgggggggg gggaaagcca cgttgtgtct caaaatctct
     9841 gatgttacat tgcacaagat atgcaacaga aactataaaa atacagagaa tgaaaagaaa
     9901 cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc
     9961 aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg
    10021 aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg
    10081 gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta
    10141 actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata
    10201 aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc
    10261 tttactaccg cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa
    10321 aagccatata aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc
    10381 cctgaacagc aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa
    10441 aatatctctt ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac
    10501 ggcactgtcg caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa
    10561 aatgcggatg acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac
    10621 agctggaaaa acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct
    10681 atcctaaaag accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa
    10741 atccgtttat tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact
    10801 gcacaagtta acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat
    10861 aaatcaatct ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa
    10921 ggcaactaca gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa
    10981 ggccacaaat acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa
    11041 gaatctttat ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt
    11101 caaaaacttc tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt
    11161 atgattgagc taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct
    11221 aacacagtaa cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac
    11281 ctgttcactg actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt
    11341 tacatgcttg gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact
    11401 ggccttgtgt taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc
    11461 gctgtacctc aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga
    11521 ttctacgcag acaaacaatc aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag
    11581 aaaacatctg ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa
    11641 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
