LOCUS       sequence-404798-        8910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus helper plasmid coding for Gag/Pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW,
            Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom 
            JD
  TITLE     A pseudovirus system enables deep mutational scanning of the full 
            SARS-CoV-2 spike.
  JOURNAL   Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 
            10.1016/j.cell.2023.02.001. Epub 2023 Feb 13.
  PUBMED    36868218
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.02.001"; journalName: "Cell"; date: 
            "2023-03-16- 16"; volume: "186"; issue: "6"; pages: "1263-1278.e20"
COMMENT     Sequence Label: HDM-Hgpm2
FEATURES             Location/Qualifiers
     source          1..8910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          779..1254
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(5680..5696)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5681..5697)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5790..6184
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(6518..6536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6604..6708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6709..6777,6778..7569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6927..6946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7740..8328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8229..8248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattcta gactgccatg
     1321 ggcgcccgcg cctccgtgct gtccggcggc gagctggaca agtgggagaa gatccgcctg
     1381 cgccccggcg gcaagaagca gtacaagctg aagcacatcg tgtgggcctc ccgcgagctg
     1441 gagcgcttcg ccgtgaaccc cggcctgctg gagacctccg agggctgccg ccagatcctg
     1501 ggccagctgc agccctccct gcaaaccggc tccgaggagc tgcgctccct gtacaacacc
     1561 atcgccgtgc tgtactgcgt gcaccagcgc atcgacgtga aggacaccaa ggaggccctg
     1621 gacaagatcg aggaggagca gaacaagtcc aagaagaagg cccagcaggc cgccgccgac
     1681 accggcaaca actcccaggt gtcccagaac taccccatcg tgcagaacct gcagggccag
     1741 atggtgcacc aggccatctc cccccgcacc ctgaacgcct gggtgaaggt ggtggaggag
     1801 aaggccttct cccccgaagt catccccatg ttctccgccc tgtccgaggg cgccaccccc
     1861 caggacctga acaccatgct gaacaccgtg ggcggccacc aggccgccat gcagatgctg
     1921 aaggagacca tcaacgagga ggccgccgag tgggaccgcc tgcaccccgt gcacgccggc
     1981 cccatcgccc ccggccagat gcgcgagccc cgcggctccg acatcgccgg caccacctcc
     2041 accctgcaag agcagatcgg ctggatgacc cacaaccccc ccatccccgt gggcgagatc
     2101 tacaagcgct ggatcatcct gggcctgaac aagatcgtgc gcatgtactc ccccacctcc
     2161 atcctggaca tccgccaggg ccccaaggag cccttccgcg actacgtgga ccgcttctac
     2221 aagaccctgc gcgccgagca ggcctcccag gaggtaaaga actggatgac cgagaccctg
     2281 ctggtgcaga acgccaaccc cgactgcaag accatcctga aggccctggg ccccggcgcc
     2341 accctggagg agatgatgac cgcctgccag ggcgtgggcg gccccggcca caaggcccgc
     2401 gtgctggccg aggccatgtc ccaagtcacc aaccccgcca ccatcatgat ccagaagggc
     2461 aacttccgca accagcgcaa gaccgtgaag tgcttcaact gcggcaagga gggccacatc
     2521 gccaagaact gccgcgcccc ccgcaagaag ggctgctgga agtgcggcaa ggagggccac
     2581 cagatgaaag attgtactga gagacaggct aattttttag ggaagatctg gccttcccac
     2641 aagggaaggc cagggaattt tcttcagagc agaccagagc caacagcccc accagaagag
     2701 agcttcaggt ttggggaaga gacaacaact ccctctcaga agcaggagcc gatagacaag
     2761 gaactgtatc ctttagcttc cctcagatca ctctttggca gcgacccctc gtcacaataa
     2821 agatcggtgg ccagctgaag gaggccctgc tggacaccgg cgccgacgac accgtgctgg
     2881 aggagatgaa cctgcccggc cgctggaagc ccaagatgat cggcggcatc ggcggcttca
     2941 tcaaagtccg ccagtacgac cagatcctga tcgagatctg cggccacaag gccatcggca
     3001 ccgtgctggt gggccccacc cccgtgaaca tcatcggccg caacctgctg acccagatcg
     3061 gctgcaccct gaacttcccc atctccccca tcgagaccgt gcccgtgaag ctgaagcccg
     3121 gcatggacgg ccccaaagtc aagcagtggc ccctgaccga ggagaagatc aaggccctgg
     3181 tggagatctg caccgagatg gagaaggagg gcaagatctc caagatcggc cccgagaacc
     3241 cctacaacac ccccgtgttc gccatcaaga agaaggactc caccaagtgg cgcaagctgg
     3301 tggacttccg cgagctgaac aagcgcaccc aggacttctg ggaggtgcag ctgggcatcc
     3361 cccaccccgc cggcctgaag cagaagaagt ccgtgaccgt gctggacgtg ggcgacgcct
     3421 acttctccgt gcccctggac aaggacttcc gcaagtacac cgccttcacc atcccctcca
     3481 tcaacaacga gacccccggc atccgctacc agtacaacgt gctgccccag ggctggaagg
     3541 gctcccccgc catcttccag tgctccatga ccaagatcct ggagcccttc cgcaagcaga
     3601 accccgacat cgtgatctac cagtacatgg acgacctgta cgtgggctcc gacctggaga
     3661 tcggccagca ccgcaccaag atcgaggagc tgcgccagca cctgctgcgc tggggcttca
     3721 ccacccccga caagaagcac cagaaggagc cccccttcct gtggatgggc tacgagctgc
     3781 accccgacaa gtggaccgtg cagcccatcg tgctgcccga gaaggactcc tggaccgtga
     3841 acgacatcca gaagctggtg ggcaagctga actgggcctc ccagatctac gccggcatca
     3901 aagtccgcca gctgtgcaag ctgctgcgcg gcaccaaggc cctgaccgag gtggtgcccc
     3961 tgaccgagga ggccgagctg gagctggccg agaaccgcga gatcctgaag gagcccgtgc
     4021 acggcgtgta ctacgacccc tccaaggacc tgatcgccga gatccagaag cagggccagg
     4081 gccagtggac ctaccagatc taccaggagc ccttcaagaa cctgaagacc ggcaaatacg
     4141 cccgcatgaa gggcgcccac accaacgacg tgaagcagct gaccgaggcc gtgcagaaga
     4201 tcgccaccga gtccatcgtg atctggggca agactcccaa gttcaagctg cccatccaga
     4261 aggagacctg ggaggcctgg tggaccgagt actggcaggc cacctggatc cccgagtggg
     4321 agttcgtgaa cacccccccc ctggtgaagc tgtggtacca gctggagaag gagcccatca
     4381 tcggcgccga gaccttctac gtggacggcg ccgccaaccg cgagaccaag ctgggcaagg
     4441 ccggctacgt gaccgaccgc ggccgccaga aggtggtgcc cctgaccgac accaccaacc
     4501 agaagaccga gctgcaggcc atccacctgg ccctgcaaga ctccggcctg gaggtgaaca
     4561 tcgtgaccga ctcccagtat gcattgggca tcatccaggc ccagcccgac aagtccgagt
     4621 ccgagctggt gtcccagatc atcgagcagc tgatcaagaa ggagaaggtg tacctggcct
     4681 gggtgcccgc ccacaagggc atcggcggca acgagcaggt ggacaagctg gtgtccgccg
     4741 gcatccgcaa ggtgctgttc ctggacggca tcgacaaggc ccaggaggag cacgagaagt
     4801 accactccaa ctggcgcgcc atggcctccg acttcaacct gccccccgtg gtggccaagg
     4861 agatcgtggc ctcctgcgac aagtgccagc tgaagggcga ggccatgcac ggccaggtgg
     4921 actgctcccc cggcatctgg cagctggact gcacccacct ggagggcaag gtgatcctgg
     4981 tggccgtgca cgtggcctcc ggctacatcg aggccgaggt gatccccgcc gagaccggcc
     5041 aggagaccgc ctacttcctg ctgaagctgg ccggccgctg gcccgtgaag accgtgcaca
     5101 ccgacaacgg ctccaacttc acctccacca ccgtgaaggc cgcctgctgg tgggccggca
     5161 tcaagcagga gttcggcatc ccctacaacc cccagtccca gggcgtgatc gagtccatga
     5221 acaaggagct gaagaagatc atcggccaag tccgcgacca ggccgagcac ctgaagaccg
     5281 ccgtgcagat ggccgtgttc atccacaact tcaagcgcaa gggcggcatc ggcggctact
     5341 ccgccggcga gcgcatcgtg gacatcatcg ccaccgacat ccagaccaag gagctgcaga
     5401 agcagatcac caagatccag aacttccgcg tgtactaccg cgactcccgc gaccccgtgt
     5461 ggaagggccc cgccaagctg ctgtggaagg gcgagggcgc cgtggtgatc caggacaact
     5521 ccgacatcaa ggtggtgccc cgccgcaagg ccaagatcat ccgcgactac ggcaagcaga
     5581 tggccggcga cgactgcgtg gcctcccgcc aggacgagga ctaacacatg gaaaagatta
     5641 gtaaaacacc ataggccgct ctagaggatc caagcttatc gataccgtcg acctcgaggg
     5701 cccagatcta attcacccca ccagtgcagg ctgcctatca gaaagtggtg gctggtgtgg
     5761 ctaatgccct ggcccacaag tatcactaag ctcgctttct tgctgtccaa tttctattaa
     5821 aggttccttt gttccctaag tccaactact aaactggggg atattatgaa gggccttgag
     5881 catctggatt ctgcctaata aaaaacattt attttcattg caatgatgta tttaaattat
     5941 ttctgaatat tttactaaaa agggaatgtg ggaggtcagt gcatttaaaa cataaagaaa
     6001 tgaagagcta gttcaaacct tgggaaaata cactatatct taaactccat gaaagaaggt
     6061 gaggctgcaa acagctaatg cacattggca acagcccctg atgcctatgc cttattcatc
     6121 cctcagaaaa ggattcaagt agaggcttga tttggaggtt aaagttttgc tatgctgtat
     6181 tttacattac ttattgtttt agctgtcctc atgaatgtct tttcactacc catttgctta
     6241 tcctgcatct ctcagccttg actccactca gttctcttgc ttagagatac cacctttccc
     6301 ctgaagtgtt ccttccatgt tttacggcga gatggtttct cctcgcctgg ccactcagcc
     6361 ttagttgtct ctgttgtctt atagaggtct acttgaagaa ggaaaaacag ggggcatggt
     6421 ttgactgtcc tgtgagccct tcttccctgc ctcccccact cacagtgacc cggaatccct
     6481 cgacatggca gtctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6541 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8341 ggagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     8401 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     8461 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     8521 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     8581 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc
     8641 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     8701 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     8761 atcataatgg ggaaggccat ccagcctcgc gtcggggagc tttttgcaaa agcctaggcc
     8821 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     8881 tgcataaata aaaaaaatta gtcagccatg
//
