LOCUS       sequence-404805-        4248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 Module 3 destination vector (reverse orientation MoClo 
            insert).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-3-Reverse
FEATURES             Location/Qualifiers
     source          1..4248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(86..91,92..109,110..116)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             170..850
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             1170..1475
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1354..1373
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  1593..2360
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     promoter        2363..2434
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3502..4090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3991..4010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4244..4248,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt caccggcgcg ccagcgtgag
       61 accggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      121 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      241 atttcagtca gtcgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
      421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      601 cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
      721 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
      781 gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc tagaggatcc
      841 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
      901 atatatactg atatgtatac ccgaagtatg tcaaaaggag gtatgctatg aagcagcgta
      961 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     1021 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     1081 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     1141 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     1201 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     1261 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     1321 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     1381 tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     1441 aaaacgccat taacctgatg ttctggggaa tataaggtct ctctccgagc tccccatttt
     1501 gcaggtgtga tgaaaagcct tagggtcagg tggcactttt cggggaaatg tgcgatgaac
     1561 ccctatttgt ttatttttct aaatacattc aaggtgatgc tgccaactta ctgatttagt
     1621 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
     1681 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc
     1741 tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt
     1801 acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc
     1861 attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg
     1921 taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt
     1981 ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc
     2041 ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta
     2101 tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg
     2161 aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc
     2221 ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct
     2281 caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc
     2341 aataagttgg agtcattacc tacattcaaa tatgtatccg ctaatgagac aataaccctg
     2401 ataaatgctt caattatatt gaaaaaggaa gagtatgagt atccaacatt tccgtgtcgc
     2461 ccttattccc ttttttgcgg ccttctgcct tcctgttttt gctcacccag aaacgctggt
     2521 gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg ggttacattg aactggatct
     2581 caacagcggc aaaatcttag agagttttcg ccccgaagaa cgcttcccaa tgatgagcac
     2641 tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt gatgctgggc aagagcaact
     2701 cggtcgccgc atacactatt ctcagaatga cttggttgag tattcaccag tcacagaaaa
     2761 gcatcttacg gatggcatga cagtaagaga attatgtagt gctgccataa ccatgagtga
     2821 taacactgcg gccaacttac ttctgacaac gattggagga ccgaaggagc taaccgcttt
     2881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caactctccg
     3001 caaactatta actggcgaac tacttactct tgcttcacgc cagcaactca ttgactggat
     3061 ggaggcggac aaagttgcag gaccacttct gcgctcggca cttccggctg gctggtttat
     3121 tgctgataaa tctggagccg gtgagcgtgg ctctcgcggt atcattgcag cactggggcc
     3181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3301 agacgaaggg aaataaatag tagcccgcct gatgtgcggg cttttttttg gtggtggtta
     3361 ccagtgtcgc ttagctaccg taggtcctct taggcggtcc gattagcgac atagtcatca
     3421 atgccttgac ggcaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc tactaactct
     3601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtattaccg ggttggactc
     3781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgctcaca
     3841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcga
//
