LOCUS       sequence-404810-        3488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 Module 5T destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-5T
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(86..91,92..109,110..116)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             170..850
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             1170..1475
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1354..1373
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        1553..1657
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2742..3330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3231..3250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3484..3488,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cctcggcgcg ccggagtgag
       61 accggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      121 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      241 atttcagtca gtcgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
      421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      601 cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
      721 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
      781 gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc tagaggatcc
      841 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
      901 atatatactg atatgtatac ccgaagtatg tcaaaaggag gtatgctatg aagcagcgta
      961 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     1021 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     1081 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     1141 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     1201 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     1261 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     1321 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     1381 tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     1441 aaaacgccat taacctgatg ttctggggaa tataaggtct ctcgctgagc tcagtgtttt
     1501 gcaggtgtga tgaaaagcct tagggtcagg tggcactttt cggggaaatg tgcgatgaac
     1561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctaatga gacaataacc
     1621 ctgataaatg cttcaattat attgaaaaag gaagagtatt gaaaaaggaa gagtatgagt
     1681 atccaacatt tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct tcctgttttt
     1741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg
     1801 ggttacattg aactggatct caacagcggc aaaatcttag agagttttcg ccccgaagaa
     1861 cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt
     1921 gatgctgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1981 tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgtagt
     2041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga
     2101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2221 gcaatggcaa caactctccg caaactatta actggcgaac tacttactct tgcttcacgc
     2281 cagcaactca ttgactggat ggaggcggac aaagttgcag gaccacttct gcgctcggca
     2341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ctctcgcggt
     2401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2521 attaagcatt ggtaactgtc agacgaaggg aaataaatag tagcccgcct gatgtgcggg
     2581 cttttttttg gtggtggtta ccagtgtcgc ttagctaccg taggtcctct taggcggtcc
     2641 gattagcgac atagtcatca atgccttgac ggcaaaatcc cttaacgtga gttttcgttc
     2701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2821 gatcaagagc tactaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2881 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3061 acggggggtt cgtgctcaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3421 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3481 cgcagcga
//
