LOCUS       sequence-404811-        3630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T16H2 promoter and native 5'UTR (approximately 1 kb upstream of 
            start codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pSB278 - pL0_pT16H2 (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     416..433
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1930..1948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2016..2120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2342..3133
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3304..3630,1..262)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
       61 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      421 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      481 gccgattcat taatcactct gtggtctcag gagagtaatc tcattgatgt gatgggcggt
      541 ggtatggtaa tgctattttt aatttgatat attttattaa taaaatttaa ggttaatata
      601 aaaaaagtac cttgaacttt gtccattatg tcaattaagc acctaatctt tgaaaaagaa
      661 aattaaggaa taattttgct ttttcgataa aataaattga acgaaaactg atcagaaatg
      721 agtaatgtat gttatgccct aataaaattt ggaaaactta aatttatcct tttataggag
      781 gaaagcttgt catcaaaatg gagggaagaa atataattaa aaaaaaattc tcttccaaga
      841 ccatcaaaat acccaattac taccaaatcc tatcagcaga aacaaatggc atgagtttct
      901 ttctcttcct tccccagacc cttaaaaacc atataccaaa gctttcactt ccttctccaa
      961 atcctcaaaa agcatataaa agtttcataa caccattatt gtgacttaaa aaaccattgt
     1021 tttgcttgca atcaacttta atgggtaaga aatattatag agattaaaaa gttgtaactt
     1081 ctaaaatata tgaaattatc aaaaagatat tctggggaac gattaataca aaaaaattac
     1141 catgaacttt gtccattgtc agattattct ttttgacaat atcacaatgt tttttattat
     1201 atttttgatg accagctccc ctcctatgca aggataaaag ttggttttcc aaattttatt
     1261 agggtagaac agaaactact catttctgat cagtttccgt tcaatgcagt ttactagaaa
     1321 agaaaaatta ttctcttgtt attttgaaag attgaatact taattgatat aatgacaaaa
     1381 ttcaagatac tgcttttgta ttaacagtaa aatttatcaa aagaaaaaaa ttcttaaggg
     1441 aaaagataaa aattaaaaaa atatatatta taaaagaaat agtataaaga atagtataga
     1501 aaaatggtag agatgatcca gagtggtggg agcctagaaa tacaagtaat tctatgggga
     1561 tcaatatcaa tcaaactaat tgtgtgttag aataggataa aaatgtttat ttatggaaaa
     1621 actacttata tccatagaag gaaaaaacta taattctctc tctctctctt ttttttcccc
     1681 ctaattttac tctttttgag aaatctatga ttctcgtgtg gaaaataaag gtgtttattt
     1741 ctcaataaaa gccattccat gtttaagcaa ataaacaaca gccacacagc tgaaagtatc
     1801 tttcttcaaa tctataaaag ccattccatc ttactagtgt taagcaaact acacagcaaa
     1861 aatcttgatt ttggtcaact ttctctacct aatgtgagac cacgaagtgg ctcttcagtg
     1921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2101 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2161 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
     2221 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2281 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2341 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2401 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2461 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2521 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2581 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2641 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2701 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2761 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2821 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2881 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2941 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3001 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3061 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     3121 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3181 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3241 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3601 taaggcgcag cggtcgggct gaacgggggg
//
