LOCUS       sequence-404815-        3261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T3R promoter and native 5'UTR (approximately 1 kb upstream of start 
            codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN1224 - pL0_pT3R (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(18..606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(98..117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(777..1568)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1790..1894)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1962..1980
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3108..3125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
       61 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      121 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      181 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      421 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
      781 ttgccgacta ccttggtgat ctcgcctttt acgtaatgga caaattcttc caactgatct
      841 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
      901 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
      961 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1021 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1081 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1141 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1201 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1261 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1321 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1381 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc gctgtgtggc
     1441 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     1501 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     1561 tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1621 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1681 atagactgta ccccaaaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     1741 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata ctcttccttt
     1801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1921 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1981 cctttcgtcc actgaagagc cacttcgtgg tctcacattt tcactagaca ttaaggaaaa
     2041 gacaagcaaa gccaaacgaa tggactgaag cctataattg atttctgaag agttcttata
     2101 atagtgccaa aagaaaacac ttacggtctg tctttaaaga ttttactgag gagaaaaata
     2161 tctttggggg tggttgggga tgggaattag tttagggttg agagattggg aatgagaatg
     2221 aattttattt ccacatatga agagaggaga caccgagata aggataaggt aaggaaggcc
     2281 agcactgggt ccctaatccg ctctattacc attcttagtt atggagaaaa cagttttggc
     2341 aaaggaaaaa attgaatatc attttaaatt tgactacttt aagaatatat ataaatatat
     2401 ataaggaaaa ttatttctcg gatataaaac gaattatgat ggtattaata atattttcta
     2461 tggtgacgat aaaacattct ttttcattat tttctttttt cattttcata ccaacatttg
     2521 ccatccaaga aaaactctac ttcacatttt ttttaagtta cccaacaata cataattttt
     2581 ttttctatga gaaataacta taactgtgtc taaactattt acaaaaaaat aatcataccc
     2641 taaatctttt ttaacttctt ttaccctcta actacgtaca gaaagagtgc cttaatgcat
     2701 tagtggtttg aactccagtc atttttgctt aaaatttatc atgcgcttga cacgtatatt
     2761 tttcttggca aaacagtcaa tcaaatcaat caattatcct aaatggtatt aggattataa
     2821 ctaaagaaga aatcgccctt tttgattagc ttttttgcat atatttataa acatgtatat
     2881 atatatatat gtatatatat ttttttgctg aatggctggc aaataaccca attttatcgt
     2941 attagttccg agtaactcat caatccgaat tccaaaagct gtttgaaatc aggccgatga
     3001 ttattgaaat ttaaggttca atacatgctc ctgagaccac agagtgatta atgaatcggc
     3061 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3241 aaggccagga accgtaaaaa g
//
