LOCUS       sequence-404818-        3253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NMT promoter and native 5'UTR (approximately 1 kb upstream of start 
            codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN1214 - pL0_pNMT (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1089..1677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1578..1597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1831..1848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2968..2986)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3054..3158
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccatt acgtaaaagg cgagatcacc
      901 aaggtagtcg gcaaataact gtcagaccaa gtttactcat atatacttta gattgattta
      961 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1201 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1861 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt
     1921 ctcaggagtg gctctgatac cactgttacg ccaatccggc aaccaaacaa gcctatttac
     1981 tattacaaac cttttctacc ttactattac agattccaaa taagctaaag aagagacaga
     2041 aaaattaaca gatctaatct attacaatcg gtctgcttcc tttctttctt cccgcgtcag
     2101 agtcaggtac aacagataag atagataggt tagtaataca gaattgaacg aaagctgtaa
     2161 ccttacctcg agagctttca ggtctgggtc agagcagcag agggaagccg aacgagctct
     2221 gcgagttcgg tttttgtgtg tgcgacgatg aacagccaca gaatgttctt ccagtgagga
     2281 acaatggaga atcccttaga tagccaagta gcctcaagac aaatagctct ctatctctct
     2341 ctcagcaaag ccctttcctc tctcaaccaa gccacacgca cggtaccgag aaggtcaacc
     2401 ggcacggact atcggagaag gagtgccgaa gggtcgagaa gaaaacgaac agacagattg
     2461 gttcgaaaga agagagagat aagacaaaag gtcagagaga gtgtggaggt agcgacggct
     2521 caacagaaga aatgagggat tagggcaggc tggagaaaaa aacgaacaat atatagccga
     2581 gtcacttgag tcgggtcagg ttgaacccgg cccgcccggc atcaaggcca gccagcccac
     2641 tccactctaa tgaggcccac aaggttgttc atcattggga ttaaaagggg ctgaagagaa
     2701 aagtgaaaaa tgtgaaaatg gcccaatagg ccaatagacg ttaaaattta tttttaataa
     2761 aaaagtataa cggaaaaaag ttaataaaat tttacatttt ttacaatatg taactaaaaa
     2821 aacaaaaaca gaacggttgg agtactacta gctttgtatt tatggtagac agatagcctt
     2881 catttcatca cttgtcatct gtgaggtagc aaaacataca acaatacaaa tgtgagacca
     2941 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3001 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3061 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca
     3181 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     3241 actgtttttt ttt
//
