LOCUS       sequence-404820-        3249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  D4H promoter and native 5'UTR (approximately 1 kb upstream of start 
            codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN1198 - pL0_pD4H (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(577..595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        663..767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             989..1780
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1951..2539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2440..2459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2693..2710
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttctaaggt ctaagattga agtgaagagt cttagagagt gtttagggaa ttgttttaga
       61 gagagaaaat tttcacatga aaataaaatt tttaaaattt tttcgagtta tccttattaa
      121 tttcctaatt gtctttatta actctctttt ttaagaaggc atggagagaa atttgaattt
      181 tttacgctat aatttgattt tagcttattc tgaatattat gctcctaaaa gtatagttat
      241 tattgtctat aaaggattcc aatcatatat gacattttgg gtaaccatct atgtaacata
      301 ttaatataat atcttttatt ataaaaattt aataaaaaat aaatattaag gaaaaaaata
      361 aattataaaa aaaatatatt atagaagaaa attttaaaca gataatacgg ttattatatt
      421 tgactggcag atttagactg tccgcagtct gtaatcctat aaaaatccta agtcttggcc
      481 aattgtgata tcatctcata gtttctgttt tcttccaaat tctgagcaag aaagaaaaat
      541 gtgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat
      601 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      661 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca
      781 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
      841 cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
      901 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
      961 cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac
     1021 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     1081 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct
     1141 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     1201 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     1261 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     1321 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     1381 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     1441 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     1501 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     1561 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     1621 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     1681 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     1741 ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc
     1801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2701 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2761 gattcattaa tcactctgtg gtctcaggag tatttcttct cactaataat aattaatttc
     2821 atattgttca cgaaaacata atttataatc atattaataa taaaaattat aacataaaat
     2881 attttaaaca actagttaca ctcattctac atataaaaaa ttaaataaca attttgataa
     2941 acatttatga acaaaaaaaa tgaaatatat aataaaaaaa aaatttaatt cagtcatttt
     3001 tgcccaggct ggagaaattc aaaggtgatt ttggcccagc ctggagaatg tttgcaggca
     3061 ctttctctag actgggactt tctccaggtt gggtgaaggt gcaatttttg ggcaaaaaaa
     3121 agtgaatcta aaattacgat ttctttttca aattaaatgc atattttcat gatttaaacc
     3181 aataaatttc taaataaatg agaatagatt gaagaacaaa cattttgttt tgaattttga
     3241 cggtcggat
//
