LOCUS       sequence-404824-       11517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for the T16H2 promoter from C. roseus with firefly 
            luciferase readout and Renilla luciferase normalization in a plant 
            binary vector for Agrobacterium-mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Cole-Osborn LF, Soens N, Bernal-Franco D, Prifti O, Cram EJ, 
            Lee-Parsons CWT
  TITLE     Identification of DELLA and GID1 genes in Catharanthus roseus and 
            their potential role in regulating vindoline biosynthesis.
  JOURNAL   Plant Mol Biol. 2025 Jun 5;115(3):72. doi: 
            10.1007/s11103-025-01599-1.
  PUBMED    40471459
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2025-06-5"; volume: "115(3):72. doi"; pages: " 
            10.1007/s11103-025-01599-1"
COMMENT     Sequence Label: pSB284 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT16H2
FEATURES             Location/Qualifiers
     source          1..11517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     167..185
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    1693..1717
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1997..2626
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3055..4128
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4194..4388
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        4450..4554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4555..5349
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6674..7262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7163..7182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7398..7422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        7587..7766
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7802..7857
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      8911..9163
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(9212..9919)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          join(11436..11517,1..107)
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 gctatatact acattctttt attttgaaaa aaatatttga aatattatat tactactaat
       61 taatgataat tattatatat atatcaaagg tagaagcaga aacttacctc gatatgtgca
      121 tctgtaaaag caattgttcc aggaaccagg gcgtatctct tcatagcctt atgcagttgc
      181 tctccagcgg ttccatcctc tagaggatag aatggcgccg ggcctttctt tatgtttttg
      241 gcgtcctcca ttaggtagag aaagttgacc aaaatcaaga tttttgctgt gtagtttgct
      301 taacactagt aagatggaat ggcttttata gatttgaaga aagatacttt cagctgtgtg
      361 gctgttgttt atttgcttaa acatggaatg gcttttattg agaaataaac acctttattt
      421 tccacacgag aatcatagat ttctcaaaaa gagtaaaatt agggggaaaa aaaagagaga
      481 gagagagaat tatagttttt tccttctatg gatataagta gtttttccat aaataaacat
      541 ttttatccta ttctaacaca caattagttt gattgatatt gatccccata gaattacttg
      601 tatttctagg ctcccaccac tctggatcat ctctaccatt tttctatact attctttata
      661 ctatttcttt tataatatat atttttttaa tttttatctt ttcccttaag aatttttttc
      721 ttttgataaa ttttactgtt aatacaaaag cagtatcttg aattttgtca ttatatcaat
      781 taagtattca atctttcaaa ataacaagag aataattttt cttttctagt aaactgcatt
      841 gaacggaaac tgatcagaaa tgagtagttt ctgttctacc ctaataaaat ttggaaaacc
      901 aacttttatc cttgcatagg aggggagctg gtcatcaaaa atataataaa aaacattgtg
      961 atattgtcaa aaagaataat ctgacaatgg acaaagttca tggtaatttt tttgtattaa
     1021 tcgttcccca gaatatcttt ttgataattt catatatttt agaagttaca actttttaat
     1081 ctctataata tttcttaccc attaaagttg attgcaagca aaacaatggt tttttaagtc
     1141 acaataatgg tgttatgaaa cttttatatg ctttttgagg atttggagaa ggaagtgaaa
     1201 gctttggtat atggttttta agggtctggg gaaggaagag aaagaaactc atgccatttg
     1261 tttctgctga taggatttgg tagtaattgg gtattttgat ggtcttggaa gagaattttt
     1321 ttttaattat atttcttccc tccattttga tgacaagctt tcctcctata aaaggataaa
     1381 tttaagtttt ccaaatttta ttagggcata acatacatta ctcatttctg atcagttttc
     1441 gttcaattta ttttatcgaa aaagcaaaat tattccttaa ttttcttttt caaagattag
     1501 gtgcttaatt gacataatgg acaaagttca aggtactttt tttatattaa ccttaaattt
     1561 tattaataaa atatatcaaa ttaaaaatag cattaccata ccaccgccca tcacatcaat
     1621 gagattactc tccttacgag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa
     1681 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     1741 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     1801 cagccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1861 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1921 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1981 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     2041 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     2101 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     2161 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     2221 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     2281 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     2341 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     2401 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     2461 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     2521 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     2581 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     2641 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     2701 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     2761 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2821 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2881 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2941 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     3001 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     3061 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     3121 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     3181 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     3241 ggtgcgtcgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     3301 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     3361 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     3421 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     3481 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     3541 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     3601 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     3661 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     3721 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     3781 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     3841 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     3901 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     3961 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     4021 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     4081 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     4141 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat
     4201 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     4261 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     4321 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     4381 tgtgcataac tgtctggcca gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg
     4441 ctccgagtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct
     4561 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
     4621 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
     4681 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
     4741 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
     4801 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
     4861 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
     4921 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
     4981 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac
     5041 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
     5101 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
     5161 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
     5221 ttctgcgtcc ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
     5281 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
     5341 ttgttttagc tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5401 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5461 taacgtgagt tttcgttcta gctgtaacct cgaagcgttt cacttgtaac aacgattgag
     5521 aatttttgtc ataaaattga aatacttggt tcgcattttt gtcatccgcg gtcagccgca
     5581 attctgacga actgcccatt tagctggaga tgattgtaca tccttcacgt gaaaatttct
     5641 caagcgctgt gaacaagggt tcagatttta gattgaaagg tgagccgttg aaacacgttc
     5701 ttcttgtcga tgacgacgtc gctatgcggc atcttattat tgaatacctt acgatccacg
     5761 ccttcaaagt gaccgcggta gccgacagca cccagttcac aagagtactc tcttccgcga
     5821 cggtcgatgt cgtggttgtt gatctagatt taggtcgtga agatgggctc gagatcgttc
     5881 gtaatctggc ggcaaagtct gatattccaa tcataattat cagtggcgac cgccttgagg
     5941 agacggataa agttgttgca ctcgagctag gagcaagtga ttttatcgct aagccgttca
     6001 gtatcagaga gtttctagca cgcattcggg ttgccttgcg cgtgcgcccc aacgttgtcc
     6061 gctccaaaga ccgacggtct ttttgtttta ctgactggac acttaatctc aggcaacgtc
     6121 gcttgatgtc cgaagctggc ggtgaggtga aacttacggc aggtgagttc aatcttctcc
     6181 tcgcgttttt agagaaaccc cgcgacgttc tatcgcgcga gcaacttctc attgccagtc
     6241 gagtacgcga cgaggaggtt tatgacagga gtatagatgt tctcattttg aggctgcgcc
     6301 gcaaacttga ggcggatccg tcaagccctc aactgataaa aacagcaaga ggtgccggtt
     6361 atttctttga cgcggacgtg caggtttcgc acggggggac gatggcagcc tgagccaatt
     6421 gcatttgcct cttaattatc tggctcaaag ggtgactgag gagtaagcga tgtgcccatc
     6481 acactgacca ccaagacggg ggttcgtttt gaagatcacc cttcagaccc aaggccagtg
     6541 tgcgttgatc gcggccacac tgcaaattca catactgtcg ataaaggggc gttggacgag
     6601 aacccattcg tcaggatact ggctcgtgat ctcactgagc gtcagacccc gtagaaaaga
     6661 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6721 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6781 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6841 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6901 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6961 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7021 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7081 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7141 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7201 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7261 aaaacgccag caacgcggcc tttttacggt tcctgctcgg atctgttgga ccggacagta
     7321 gtcatggttg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     7381 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     7441 cttaataaca cattgcggac gtttttaatg tactggggtt gaacactctg tgccgaattc
     7501 ggatccggag agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac
     7561 aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg
     7621 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
     7681 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
     7741 cataaattcc cctcggtatc caattagagt ctcatattca ctctcatact gtatttttac
     7801 aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat
     7861 gacttctaag gtttatgatc ctgaacaaag aaagagaatg attactggac ctcaatggtg
     7921 ggctagatgt aagcaaatga atgttcttga ttcttttatt aattattatg attctgaaaa
     7981 gcatgctgaa aatgctgtta tttttcttca tggaaatgct gcttcttctt atctttggag
     8041 acatgttgtt ccacatattg aaccagttgc tagatgtatt attccagatc ttattggtat
     8101 gggaaagtct ggtaagtctg gtaatggttc ttatagactt cttgatcatt ataagtatct
     8161 tactgcttgg tttgaacttc ttaatcttcc aaagaagatt atttttgttg gacatgattg
     8221 gggtgcttgt ttggcttttc attattctta tgaacatcaa gataagatta aggctattgt
     8281 tcatgctgaa tctgttgttg atgttattga atcttgggat gaatggcctg atattgaaga
     8341 agatattgct ttgattaagt ctgaagaagg agaaaagatg gttttggaaa ataatttctt
     8401 tgttgaaact atgttgccat ctaagattat gagaaagctt gaaccagaag aatttgctgc
     8461 ttatcttgaa ccatttaagg aaaagggtga agttagaagg ccaactcttt cttggcctag
     8521 agaaattcct cttgttaagg gtggtaagcc tgatgttgtt caaattgtta gaaattataa
     8581 tgcttatctt agagcttctg atgatcttcc aaagatgttt attgaatctg atccaggatt
     8641 cttttctaat gctattgttg aaggtgctaa gaagtttcct aatactgaat ttgttaaggt
     8701 acttcatgct tcaacgtgta acttaagaga tactgtgtga aattttatat ttccatacat
     8761 ttgcttgacc tttgcttttt gtcaattttt ttccccttac aggttaaggg acttcatttt
     8821 tctcaagaag atgctcctga tgaaatggga aagtatatta agtcttttgt tgaaagagtt
     8881 cttaagaatg aacaatgagc ttgtcaagca gatcgttcaa acatttggca ataaagtttc
     8941 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     9001 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     9061 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     9121 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgacgctg caaggttttg
     9181 acgggtttac tagaattcga gctcagcggt cctgctgagc ctcgacatgt tgtcgcaaaa
     9241 ttcgccctgg acccgcccaa cgatttgtcg tcactgtcaa ggtttgacct gcacttcatt
     9301 tggggcccac atacaccaaa aaaatgctgc ataattctcg gggcagcaag tcggttaccc
     9361 ggccgccgtg ctggaccggg ttgaatggtg cccgtaactt tcggtagagc ggacggccaa
     9421 tactcaactt caaggaatct cacccatgcg cgccggcggg gaaccggagt tcccttcagt
     9481 gagcgttatt agttcgccgc tcggtgtgtc gtagatacta gcccctgggg cacttttgaa
     9541 atttgaataa gatttatgta atcagtcttt taggtttgac cggttctgcc gcttttttta
     9601 aaattggatt tgtaataata aaacgcaatt gtttgttatt gtggcgctct atcatagatg
     9661 tcgctataaa cctattcagc acaatatatt gttttcattt taatattgta catataagta
     9721 gtagggtaca atcagtaaat tgaacggaga atattattca taaaaatacg atagtaacgg
     9781 gtgatatatt cattagaatg aaccgaaacc ggcggtaagg atctgagcta cacatgctca
     9841 ggttttttac aacgtgcaca acagaattga aagcaaatat catgcgatca taggcttctc
     9901 gcatatctca ttaaagcagg acaagcttac aatttggact ttccgccctt cttggccttt
     9961 atgaggatct ctctgatttt tcttgcgtcg agttttccgg taagaccttt cggtacttcg
    10021 tccacaaaca caactcctcc gcgcaacttt ttcgcggttg ttacttgact ggcgacgtaa
    10081 tccacgatct ctttttccgt catcgtcttt ccgtgctcca aaacaacaac ggcggcggga
    10141 agttcaccgg cgtcatcgtc gggcagacct gccacgcccg cgtcgaagat gttggggtgt
    10201 tgtaacaata tcgattccaa ttcagcgggg gccacctgat atcctttgta tttaattaaa
    10261 gacttcaagc ggtcaactat gaagaagtgt tcgtcctcgt cccagtaagc tatgtctcca
    10321 gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg cttccggatt gtttacataa
    10381 ccggacataa tcataggtcc tctgacacat aattcgcctc tctgattaac gcccagcgtt
    10441 ttcccggtat ccagatccac aaccttcgct tcaaaaaatg gaacaacttt accgaccgcg
    10501 cccggtttat catccccctc gggtgtaatc agaatagctg atgtagtctc agtgagccca
    10561 tatccttgtc gtatccctgg aagatggaag cgttttgcaa ccgcttcccc gacttctttc
    10621 gaaagaggtg cgcccccaga agcaatttcg tgtaaattag ataaatcgta tttgtcaatc
    10681 agagtgcttt tggcgaagaa tgaaaatagg gttggtacta gcaacgcact ttgaattttg
    10741 taatcctgaa gggatcgtaa aaacagctct tcttcaaatc tatacattaa gacgactcga
    10801 aatccacata tcaaatatcc gagtgtagta aacattccaa aaccgtgatg gaatggaaca
    10861 acacttaaaa tcgcagtatc cggaatgatt tgattgccaa aaataggatc tctggcatgc
    10921 gagaatctga cgcaggcagt tctatgcgga agggccacac ccttaggtaa cccagtagat
    10981 ccagaggaat tcattatcag tgcaattgtt ttgtcacgat caaaggactc tggtacaaaa
    11041 tcgtattcat taaaaccggg aggtagatga gatgtgacga acgtgtacat cgactgaaat
    11101 ccctggtaat ccgttttaga atccatgata ataattttct ggattattgg taattttttt
    11161 tgcacgttca aaattttttg caaccccttt ttggaaacaa acactacggt aggctgcgaa
    11221 atgttcatac tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg cgcaactgca
    11281 actccgataa ataacgcgcc caacaccggc ataaagaatt gaagagagtt ttcactgcat
    11341 acgacgattc tgtgatttgt attcagccca tatcgtttca tagcttctgc caaccgaacg
    11401 gacatttcga agtattccgc gtacgtgatg ttcacctgca catcaacaaa ttttggtcat
    11461 atattagaaa agttataaat taaaatatac acacttataa actacagaaa agcaatt
//
