LOCUS       sequence-404829-        3354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT3G18480(F,G)
FEATURES             Location/Qualifiers
     source          1..3354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      291..879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     780..799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1033..1050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2271..2289)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2357..2461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2683..3354,1..120)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
       61 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
      121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1081 cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag gctttaagaa
     1141 tggagggtgg gtgggtatat atatggttga tggtggatcg cttcgagaga tctacaaatg
     1201 tttaattttt agtacattgc actttgggaa aaatcataaa agttgtaaaa aagagtaaaa
     1261 gacttttaaa actagtacgt tgcacttttt gttaaaagga ggaaggtttt ttgaaaattg
     1321 atttttgttt aaggaaggtg agagatttta ctgattgtga gagattttaa gctacttaaa
     1381 agtaatatgt aaacgcgttt ttgggattgg tttaatccac acatatatac aagatacaaa
     1441 gaatttaatt taatgactaa gaaaattaaa gaaattatga ataaaaaaga aatatacatc
     1501 ctaaaaatgc gaaagtaaaa taaaacaggc tatgctaaat tttaaaatat taaattcaac
     1561 atttttctat tcataaaatt tctattaaaa ttatttgtag ataagagata atatgttcaa
     1621 acattattcc tctatataag gaatcaaact tgcaaacttt aaaataaaat tttgaaaaat
     1681 atatctaatg taatattctg gttttattag taaagtaata ttacttaata ctaacaaatt
     1741 acaaagaaaa gaactgaagg aaaaaaaata aacttttaaa attttctaac cagtgaaata
     1801 aattagaatt taaacttaaa attttattaa ttaataatag tcataacaaa atttgacaaa
     1861 tgttttcaac ttagatgtgc aataaacatt gccatacacc ttgaggtgga tactgggcct
     1921 atgaatggcg caaggaatag cccattggtt attctatatc ttttggacct tacgttgtgg
     1981 taacttgctc catgtctggc tcataacaaa aaaataaata agaattacgc ttctaccacc
     2041 attaaactct aatgtatctg aactcagaag atccgatcgg agtagtaaaa attcaacaaa
     2101 acaacaataa gaaaactcat taattcattc gtcggattcg aagaagaaat cacttcccag
     2161 aaactttctt cgtatcgaag tattgcttgt gttcttcgga gaaacgagat cgggatccga
     2221 atacgagtac aaatgtgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     2281 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2341 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2401 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2461 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     2521 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     2581 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2641 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     2701 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2761 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     2821 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2881 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2941 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     3001 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     3061 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3121 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3181 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3241 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3301 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt cata
//
