LOCUS       sequence-404830-        3131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT1G71900
FEATURES             Location/Qualifiers
     source          1..3131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      225..813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     714..733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     967..984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1982..2000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2068..2172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2394..3131,1..54)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
       61 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      121 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      181 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      241 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      301 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      361 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      421 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      481 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      541 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      601 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      661 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      721 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      781 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      841 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      901 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      961 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     1021 cccgcgcgtt ggccgattca ttaatcactc tgtggtctca gcttaagctt tttcgctctg
     1081 ttttctggtc cactgcgata actgcaaacc agagaatcaa ccttttttcc ttatctcgac
     1141 ttattatgaa catggaccat ggttctgtct aacacttaca aatggttcaa gaagatcgaa
     1201 gaaggaaaga ggagcagcga ggatgagatc atcttctcca atatcggtaa atatgctgtg
     1261 agttcaagca cacgccatta gcacacaccc acgcctaaac ccttggtgtg acagttaaaa
     1321 ttttataaac aagagatggg ttttgcctaa attggtctta caagcatcta taactaaagg
     1381 tttcaattca agatttgcat tattttttat tgaatcttct atttttcttc cttagttggt
     1441 ttgaagcttt aaaccctttg aaaaacaaga aatggagact ttgaaagttg acgaaacaca
     1501 catgttttat tattagtcaa tagtaatttg atgaggtgga gttataacat tgatgataat
     1561 agatagtgac aacgttctat gttcatcttt tcctcaatca atcactccac cggtagatga
     1621 gatgtcaaat tgaaactgcg ttatgtcact ctcattactc tttttgtata cgtttctcat
     1681 cttatagcta atccgaaacc aatttattta aaactttata atagcataaa acctcttaac
     1741 ttcaaacaag tttcaacttt cacatatctt atagggatca tggtaaaaat ggttaaaagt
     1801 taaacctgat ttgcattaca aacagaggat tacgtgtata agggctataa actatatatt
     1861 acctaaaaaa gaaaactaga gttgacttat tgagtaataa aatcgcctat aaatatcgta
     1921 acgaaagaaa aaagatccca aacgcttgag accacgaagt ggctcttcag tggacgaaag
     1981 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2041 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2101 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2161 aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2221 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta
     2281 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2341 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
     2401 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2461 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2521 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2581 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2641 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2701 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2761 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2821 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2881 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2941 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3001 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3061 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3121 cagatcagtt g
//
