LOCUS       sequence-404832-        2835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT1G64990
FEATURES             Location/Qualifiers
     source          1..2835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      687..1275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1176..1195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1429..1446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2148..2166)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2234..2338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2560..2835,1..516)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
       61 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
      121 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
      181 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
      241 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
      301 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
      361 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
      421 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac
      481 gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt ttactcatat
      541 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      601 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      661 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      721 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      781 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      841 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      901 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      961 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1021 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1081 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1141 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1201 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1261 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1321 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1381 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1441 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1501 cattaatcac tctgtggtct cagcttgttt cttattcaat aatggtgcct gagagagtct
     1561 tatgattgtt tggcctgaca caacgaaaat caatttacta ctagtaaagg tgacttagtg
     1621 tatagcttac aattggctat ttgtttgttt ttgacgctta tgtgaaagat taaactgatc
     1681 agttcaaaat ttcttatacc ataaacgttg aattatgttt atttgtacat tgctcctact
     1741 gaggaagtaa tgatacgatg aagtcaagaa gattttcgat atttgtatgt tcgacttatt
     1801 ccacaggatc caggtttctc catttgttat ttattaacat atacaaattg tattataaaa
     1861 tgcgttgcgt gagtaacctt agtaaataag taataacaca ttcagtattg gatgctcgta
     1921 aaacaatttt gagtagtaga aagtctaacg attttgttag attgaaactg caagactagg
     1981 gagctaagaa tatgaatatc tcaatcccac caaatgtaat aaaatgttga atgaatcata
     2041 aacctctaga gtagggtctg gtggtctgat tcagttaatc aatccatatt acaagtcgat
     2101 gtggaaaccg cttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
     2161 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2221 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2281 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2341 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2401 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     2461 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2521 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     2581 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     2641 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     2701 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     2761 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     2821 gtcaccattg ttgtg
//
