LOCUS       sequence-404833-       11148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for the T3R promoter from C. roseus with firefly 
            luciferase readout and Renilla luciferase normalization in a plant 
            binary vector for Agrobacterium-mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Cole-Osborn LF, Soens N, Bernal-Franco D, Prifti O, Cram EJ, 
            Lee-Parsons CWT
  TITLE     Identification of DELLA and GID1 genes in Catharanthus roseus and 
            their potential role in regulating vindoline biosynthesis.
  JOURNAL   Plant Mol Biol. 2025 Jun 5;115(3):72. doi: 
            10.1007/s11103-025-01599-1.
  PUBMED    40471459
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2025-06-5"; volume: "115(3):72. doi"; pages: " 
            10.1007/s11103-025-01599-1"
COMMENT     Sequence Label: pUN1307 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT3R
FEATURES             Location/Qualifiers
     source          1..11148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             32..1105
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1171..1365
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1427..1531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1532..2326
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3651..4239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4140..4159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4375..4399
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4564..4743
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4779..4834
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5888..6140
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6189..6896)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          8413..8601
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     8661..8679
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    9818..9842
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10122..10751
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
       61 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
      121 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
      181 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgtcgtcga ttaggaagcc
      241 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
      301 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
      361 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
      421 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
      481 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
      541 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
      601 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
      661 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
      721 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
      781 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
      841 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
      901 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
      961 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     1021 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     1081 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     1141 aggggaaaaa ggtcgaaaaa gcttctttcc tgtggatagc acgtacattg ggaacccaaa
     1201 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     1261 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     1321 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     1381 cacagccgaa cagctgcaaa aagcgcccac atcaaggctc cgagtgcgcg gaacccctat
     1441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1501 aatgcttcaa taatattgaa aaaggaagag tatggctaaa atgagaatat caccggaatt
     1561 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     1621 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     1681 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     1741 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     1801 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     1861 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     1921 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     1981 tctggccgat gtggattgcg aaaactggga agaggacact ccatttaaag atccgcgcga
     2041 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     2101 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     2161 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgctcaggga
     2221 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     2281 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagctgt cagaccaagt
     2341 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2401 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttctagct
     2461 gtaacctcga agcgtttcac ttgtaacaac gattgagaat ttttgtcata aaattgaaat
     2521 acttggttcg catttttgtc atccgcggtc agccgcaatt ctgacgaact gcccatttag
     2581 ctggagatga ttgtacatcc ttcacgtgaa aatttctcaa gcgctgtgaa caagggttca
     2641 gattttagat tgaaaggtga gccgttgaaa cacgttcttc ttgtcgatga cgacgtcgct
     2701 atgcggcatc ttattattga ataccttacg atccacgcct tcaaagtgac cgcggtagcc
     2761 gacagcaccc agttcacaag agtactctct tccgcgacgg tcgatgtcgt ggttgttgat
     2821 ctagatttag gtcgtgaaga tgggctcgag atcgttcgta atctggcggc aaagtctgat
     2881 attccaatca taattatcag tggcgaccgc cttgaggaga cggataaagt tgttgcactc
     2941 gagctaggag caagtgattt tatcgctaag ccgttcagta tcagagagtt tctagcacgc
     3001 attcgggttg ccttgcgcgt gcgccccaac gttgtccgct ccaaagaccg acggtctttt
     3061 tgttttactg actggacact taatctcagg caacgtcgct tgatgtccga agctggcggt
     3121 gaggtgaaac ttacggcagg tgagttcaat cttctcctcg cgtttttaga gaaaccccgc
     3181 gacgttctat cgcgcgagca acttctcatt gccagtcgag tacgcgacga ggaggtttat
     3241 gacaggagta tagatgttct cattttgagg ctgcgccgca aacttgaggc ggatccgtca
     3301 agccctcaac tgataaaaac agcaagaggt gccggttatt tctttgacgc ggacgtgcag
     3361 gtttcgcacg gggggacgat ggcagcctga gccaattgca tttgcctctt aattatctgg
     3421 ctcaaagggt gactgaggag taagcgatgt gcccatcaca ctgaccacca agacgggggt
     3481 tcgttttgaa gatcaccctt cagacccaag gccagtgtgc gttgatcgcg gccacactgc
     3541 aaattcacat actgtcgata aaggggcgtt ggacgagaac ccattcgtca ggatactggc
     3601 tcgtgatctc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4261 ttacggttcc tgctcggatc tgttggaccg gacagtagtc atggttgatg ggctgcctgt
     4321 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
     4381 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
     4441 tttaatgtac tggggttgaa cactctgtgc cgaattcgga tccggagagc ggagaattaa
     4501 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     4561 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     4621 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     4681 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     4741 ttagagtctc atattcactc tcatactgta tttttacaac aattaccaac aacaacaaac
     4801 aacaaacaac attacaatta ctatttacaa ttacaatgac ttctaaggtt tatgatcctg
     4861 aacaaagaaa gagaatgatt actggacctc aatggtgggc tagatgtaag caaatgaatg
     4921 ttcttgattc ttttattaat tattatgatt ctgaaaagca tgctgaaaat gctgttattt
     4981 ttcttcatgg aaatgctgct tcttcttatc tttggagaca tgttgttcca catattgaac
     5041 cagttgctag atgtattatt ccagatctta ttggtatggg aaagtctggt aagtctggta
     5101 atggttctta tagacttctt gatcattata agtatcttac tgcttggttt gaacttctta
     5161 atcttccaaa gaagattatt tttgttggac atgattgggg tgcttgtttg gcttttcatt
     5221 attcttatga acatcaagat aagattaagg ctattgttca tgctgaatct gttgttgatg
     5281 ttattgaatc ttgggatgaa tggcctgata ttgaagaaga tattgctttg attaagtctg
     5341 aagaaggaga aaagatggtt ttggaaaata atttctttgt tgaaactatg ttgccatcta
     5401 agattatgag aaagcttgaa ccagaagaat ttgctgctta tcttgaacca tttaaggaaa
     5461 agggtgaagt tagaaggcca actctttctt ggcctagaga aattcctctt gttaagggtg
     5521 gtaagcctga tgttgttcaa attgttagaa attataatgc ttatcttaga gcttctgatg
     5581 atcttccaaa gatgtttatt gaatctgatc caggattctt ttctaatgct attgttgaag
     5641 gtgctaagaa gtttcctaat actgaatttg ttaaggtact tcatgcttca acgtgtaact
     5701 taagagatac tgtgtgaaat tttatatttc catacatttg cttgaccttt gctttttgtc
     5761 aatttttttc cccttacagg ttaagggact tcatttttct caagaagatg ctcctgatga
     5821 aatgggaaag tatattaagt cttttgttga aagagttctt aagaatgaac aatgagcttg
     5881 tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     5941 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     6001 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     6061 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     6121 gtcatctatg ttactagatc gacgctgcaa ggttttgacg ggtttactag aattcgagct
     6181 cagcggtcct gctgagcctc gacatgttgt cgcaaaattc gccctggacc cgcccaacga
     6241 tttgtcgtca ctgtcaaggt ttgacctgca cttcatttgg ggcccacata caccaaaaaa
     6301 atgctgcata attctcgggg cagcaagtcg gttacccggc cgccgtgctg gaccgggttg
     6361 aatggtgccc gtaactttcg gtagagcgga cggccaatac tcaacttcaa ggaatctcac
     6421 ccatgcgcgc cggcggggaa ccggagttcc cttcagtgag cgttattagt tcgccgctcg
     6481 gtgtgtcgta gatactagcc cctggggcac ttttgaaatt tgaataagat ttatgtaatc
     6541 agtcttttag gtttgaccgg ttctgccgct ttttttaaaa ttggatttgt aataataaaa
     6601 cgcaattgtt tgttattgtg gcgctctatc atagatgtcg ctataaacct attcagcaca
     6661 atatattgtt ttcattttaa tattgtacat ataagtagta gggtacaatc agtaaattga
     6721 acggagaata ttattcataa aaatacgata gtaacgggtg atatattcat tagaatgaac
     6781 cgaaaccggc ggtaaggatc tgagctacac atgctcaggt tttttacaac gtgcacaaca
     6841 gaattgaaag caaatatcat gcgatcatag gcttctcgca tatctcatta aagcaggaca
     6901 agcttacaat ttggactttc cgcccttctt ggcctttatg aggatctctc tgatttttct
     6961 tgcgtcgagt tttccggtaa gacctttcgg tacttcgtcc acaaacacaa ctcctccgcg
     7021 caactttttc gcggttgtta cttgactggc gacgtaatcc acgatctctt tttccgtcat
     7081 cgtctttccg tgctccaaaa caacaacggc ggcgggaagt tcaccggcgt catcgtcggg
     7141 cagacctgcc acgcccgcgt cgaagatgtt ggggtgttgt aacaatatcg attccaattc
     7201 agcgggggcc acctgatatc ctttgtattt aattaaagac ttcaagcggt caactatgaa
     7261 gaagtgttcg tcctcgtccc agtaagctat gtctccagaa tgtagccatc catccttgtc
     7321 aatcaaggcg ttggtcgctt ccggattgtt tacataaccg gacataatca taggtcctct
     7381 gacacataat tcgcctctct gattaacgcc cagcgttttc ccggtatcca gatccacaac
     7441 cttcgcttca aaaaatggaa caactttacc gaccgcgccc ggtttatcat ccccctcggg
     7501 tgtaatcaga atagctgatg tagtctcagt gagcccatat ccttgtcgta tccctggaag
     7561 atggaagcgt tttgcaaccg cttccccgac ttctttcgaa agaggtgcgc ccccagaagc
     7621 aatttcgtgt aaattagata aatcgtattt gtcaatcaga gtgcttttgg cgaagaatga
     7681 aaatagggtt ggtactagca acgcactttg aattttgtaa tcctgaaggg atcgtaaaaa
     7741 cagctcttct tcaaatctat acattaagac gactcgaaat ccacatatca aatatccgag
     7801 tgtagtaaac attccaaaac cgtgatggaa tggaacaaca cttaaaatcg cagtatccgg
     7861 aatgatttga ttgccaaaaa taggatctct ggcatgcgag aatctgacgc aggcagttct
     7921 atgcggaagg gccacaccct taggtaaccc agtagatcca gaggaattca ttatcagtgc
     7981 aattgttttg tcacgatcaa aggactctgg tacaaaatcg tattcattaa aaccgggagg
     8041 tagatgagat gtgacgaacg tgtacatcga ctgaaatccc tggtaatccg ttttagaatc
     8101 catgataata attttctgga ttattggtaa ttttttttgc acgttcaaaa ttttttgcaa
     8161 cccctttttg gaaacaaaca ctacggtagg ctgcgaaatg ttcatactgt tgagcaattc
     8221 acgttcatta taaatgtcgt tcgcgggcgc aactgcaact ccgataaata acgcgcccaa
     8281 caccggcata aagaattgaa gagagttttc actgcatacg acgattctgt gatttgtatt
     8341 cagcccatat cgtttcatag cttctgccaa ccgaacggac atttcgaagt attccgcgta
     8401 cgtgatgttc acctgcacat caacaaattt tggtcatata ttagaaaagt tataaattaa
     8461 aatatacaca cttataaact acagaaaagc aattgctata tactacattc ttttattttg
     8521 aaaaaaatat ttgaaatatt atattactac taattaatga taattattat atatatatca
     8581 aaggtagaag cagaaactta cctcgatatg tgcatctgta aaagcaattg ttccaggaac
     8641 cagggcgtat ctcttcatag ccttatgcag ttgctctcca gcggttccat cctctagagg
     8701 atagaatggc gccgggcctt tctttatgtt tttggcgtcc tccattttca ctagacatta
     8761 aggaaaagac aagcaaagcc aaacgaatgg actgaagcct ataattgatt tctgaagagt
     8821 tcttataata gtgccaaaag aaaacactta cggtctgtct ttaaagattt tactgaggag
     8881 aaaaatatct ttgggggtgg ttggggatgg gaattagttt agggttgaga gattgggaat
     8941 gagaatgaat tttatttcca catatgaaga gaggagacac cgagataagg ataaggtaag
     9001 gaaggccagc actgggtccc taatccgctc tattaccatt cttagttatg gagaaaacag
     9061 ttttggcaaa ggaaaaaatt gaatatcatt ttaaatttga ctactttaag aatatatata
     9121 aatatatata aggaaaatta tttctcggat ataaaacgaa ttatgatggt attaataata
     9181 ttttctatgg tgacgataaa acattctttt tcattatttt cttttttcat tttcatacca
     9241 acatttgcca tccaagaaaa actctacttc acattttttt taagttaccc aacaatacat
     9301 aatttttttt tctatgagaa ataactataa ctgtgtctaa actatttaca aaaaaataat
     9361 cataccctaa atctttttta acttctttta ccctctaact acgtacagaa agagtgcctt
     9421 aatgcattag tggtttgaac tccagtcatt tttgcttaaa atttatcatg cgcttgacac
     9481 gtatattttt cttggcaaaa cagtcaatca aatcaatcaa ttatcctaaa tggtattagg
     9541 attataacta aagaagaaat cgcccttttt gattagcttt tttgcatata tttataaaca
     9601 tgtatatata tatatatgta tatatatttt tttgctgaat ggctggcaaa taacccaatt
     9661 ttatcgtatt agttccgagt aactcatcaa tccgaattcc aaaagctgtt tgaaatcagg
     9721 ccgatgatta ttgaaattta aggttcaata catgctcctt acgaggatgc acatgtgacc
     9781 gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa
     9841 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     9901 tatccgttcg tccatttgta tgtgccagcc gtgcggctgc atgaaatcct ggccggtttg
     9961 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
    10021 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
    10081 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
    10141 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
    10201 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
    10261 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
    10321 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
    10381 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
    10441 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
    10501 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
    10561 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
    10621 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
    10681 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
    10741 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
    10801 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
    10861 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
    10921 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
    10981 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
    11041 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
    11101 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatg
//
