LOCUS       sequence-404835-        2996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT5G10630
FEATURES             Location/Qualifiers
     source          1..2996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      301..889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     790..809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1043..1060
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1923..1941)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2009..2113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2335..2996,1..130)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
       61 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
      121 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
      181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
      241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
     1141 aaagaaaaag aaaaaaagaa gtgaagtttg ttttgagtac aaaactacaa cttcaaacat
     1201 taccattatt aacgatccaa aacatatgtt aattgtttat accaaaacta ttttccatca
     1261 gattttgtct ctttgatttt tcggatcctg gcggaaaaaa aaagagaaga tttttcggat
     1321 cctggaaata tcagatatag gcccgttaaa agcccaacaa tttcattagt tattatttac
     1381 ggttttgttg tgtgttttcc atattcttac tctatccaac cgaaacccga atccggatcc
     1441 aaatccagat ccgaagcctt catatcgtcg gcatcaacag gcgacgaatt acctcaaact
     1501 caaagctaaa tttacaggcg gagaagttgg attcgaagga acgtctttcg taaatcaatt
     1561 ctgctctgta actagtttct gtaagtgttt ggatcaatat tctttgttaa gctcaaagtt
     1621 aatgaatttg gaattggatt ttcaatatca ccgttcgtaa aatgcttcag aaagatgtct
     1681 gaattctctc tttgttactc attgcttctg atcactgtcg gttttgtggg tttaagtccg
     1741 acaagtgtag gagcttttgc ttctgagtta tgtcggtttt attatcttaa ctatatatgt
     1801 gttgtgtttt tttatacaga attgaaactt aacttacata caatctctgt ttttttgttt
     1861 gcccttgtgc gcagaaacaa acgaatgtga gaccacgaag tggctcttca gtggacgaaa
     1921 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1981 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2041 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2101 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2161 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
     2221 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     2281 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     2341 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     2401 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     2461 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     2521 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     2581 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     2641 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     2701 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     2761 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     2821 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     2881 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     2941 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatca
//
