LOCUS       sequence-404836-        3421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT5G42470
FEATURES             Location/Qualifiers
     source          1..3421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      932..1520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1421..1440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1674..1691
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2979..2997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3065..3169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3391..3421,1..761)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
       61 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc
      121 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      181 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      241 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      301 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      361 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      421 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
      481 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
      541 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
      601 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
      661 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
      721 attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact
      781 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
      841 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      901 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      961 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1021 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1081 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1141 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1201 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1261 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1321 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1381 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1441 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1501 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1561 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1621 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1681 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1741 cgattcatta atcactctgt ggtctcagga gtctcatatt tcttcatcac gcacatactt
     1801 tttttatttc ttttattgta aagggtaaaa tcacatgttc actattaact taccactaaa
     1861 atgcggtagt tttggataga tattgtagaa tgtagtagat ttagaaaggg ttttccaaaa
     1921 tgtggtagat tcaacaaaat ttcttttttt ttaaaaaaaa aaatctacca cattcgagag
     1981 ttgatacttt gttagctttg ctttgtattg ataatttttt tttttttttt aattgataaa
     2041 aagaaaacta gatattgaca ttcggatttc ggatcggatt tgaatcgaag tttttcggat
     2101 tcagataatt tggatgggaa agtttattat ccattcggat tttatgacta ttcggtttgg
     2161 atgatgttgg attcagatcg gttttaggaa aaaaaattgg ttaaatttta caaaaatttg
     2221 gttaaatttt acaaaaaatt gtataattgt aacaaatatg actaaattgt tttggatatc
     2281 taggttattt cggatatttt ttggtataaa attataacta aatataacta atatttttta
     2341 aaattataac taaatatcat aatattttta gaggtataat tttattttgg gttatcgaat
     2401 atccgtttgg tttttggttc gaatcagatt cggttcgaat aattcggata gaagaagtca
     2461 ttacccattt ggagttttgt gattattcga tttggtttca aaaccggatt tttggatctg
     2521 tttcatattg atttttggat ttcggatttt atgtctactc ctaaaaaaac ccatgtagtt
     2581 ttcgttttaa gtgtagaatt tatcttttgg ttgtaagtta tatctagtct atcttttggg
     2641 acgtttggtt tcccatgttt ggattgtttg ttgtaagtcc tatgtacaac tccatcttct
     2701 atcgagaatc ttttagaagg aaaaaaaaaa ctcgtattta cgaaatttga cagtagaaaa
     2761 caaaaaccta agtacaattc aaattgacaa aaaaaaaaaa aaagtaaaaa ttcttaaaag
     2821 gggactacag taaaaattca tgttaaatct aactgctcga acgttccagt ttccaccacc
     2881 cggcgcgtga gtagtaccac cgtcgtcact ttcacaatct cccaacagag atccttccca
     2941 atgtgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt
     3001 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg
     3181 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     3241 ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     3301 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     3361 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     3421 a
//
