LOCUS       sequence-404837-        2574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT3G18480
FEATURES             Location/Qualifiers
     source          1..2574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      290..878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     779..798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1032..1049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1490..1508)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1576..1680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1902..2574,1..119)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
       61 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
      121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
      181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
      241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat cactctgtgg tctcagcttg ctctcaccct
     1141 agggacttat tcgtttttgg tcgttatgtt ctttcttgtc cttgtagtgt tccgtgggca
     1201 attctaaaac ggttgaatat ttgtacagag ggatccaaat cactcgatac atatataaag
     1261 ccacagaaaa aacttccttc agaaaactgc agaaatctaa ataagatctg gtttactttt
     1321 cttcgaaaca taaataacca tgaaaaatgc accaaaacga taacaaatcc gacaggaata
     1381 attgttgcta tccaaataca aaataaaata aaatgaaagt cgttgcttga tatctgttag
     1441 gcaacaacac cgcttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata
     1501 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1681 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1741 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     1801 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1861 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     1921 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1981 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2041 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2101 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2161 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2221 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2281 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2341 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2401 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2461 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2521 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atac
//
